A previously characterized compound microsatellite locus ARMS1, containing penta- and decameric repeat units, has been reported to reveal genetic diversity in Ascochyta rabiei (Pass.) Labr. isolates. Therefore, 37 isolates of Ascochyta rabiei collected from different states of India and 38 isolates from fifteen other countries in the world were examined for their diversity at this locus. Twenty-six alleles on the basis of size (228--451 base pairs) were detected in the world isolates examined, while 15 alleles (287--418 base pairs) were observed in isolates from the Indian subcontinent. To the best of our knowledge, this study is the first to demonstrate diversity in representative Ascochyta rabiei isolates from different parts of the world at the ARMS1 locus.
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Arenal, F., Platas, G., Martin, J., Salazar, O. & Pela´ ez, F. 1999 Evaluation of different PCR-based DNA fingerprinting techniques for assessing the genetic variability of isolates of the fungus Epicoccum nigrum. Journal of Applied Microbiology 87, 898–906.
Brave, M.P., Arie, T., Salimath, S., Muehlbauer, F.J. & Peever, T.L. 2003 Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp. Fungal Genetics and Biology 39, 151–167.
Brown, J.K.M. 1996 The choice of molecular marker methods for population genetic studies of plant pathogens. New Phytologist 133 183–195.
Bull, L.N., Pabo´n-Peña & Freimer, N.B. 1999 Compound microsatellite repeats: practical and theoretical features. Genome Research 9, 830–838.
Delmotte, F., Bucheli, E. & Shykoff, J.A. 1999 Host and parasite population structure in a natural plant-pathogen system Heredity 82, 300–308.
Doyle, J.J. & Doyle, J.L. 1987 A rapid DNA isolation procedure from small amount of fresh leaf tissue. Phytochemical Bulletin 19, 11–15.
Epplen, J.T. 1988 On simple repeated GATA/GACA sequences in animal genomes: a critical reappraisal. Journal of Heredity 19, 409–417.
Field, D. & Wills, C. 1996 Long polymorphic microsatellites in simple organisms. Proceedings of the Royal Society of London 263, 209–215.
Geistlinger, J., Maqbool, S., Kaiser, W.J. & Kahl, G. 1997a Detection of microsatellite fingerprint markers and their Mendelian inheritance in Ascochyta rabiei. Mycological Research 101, 1113–1121.
Geistlinger, J., Weising, K., Kaiser, W.J. & Kahl, G. 1997b Allelic variation at a hypervariable compound microsatellite locus in the ascomycete Ascochyta rabiei. Molecular and General Genetics 256, 298–305.
Geistlinger, J., Weising, K, Winter, P. & Kahl, G. 2000 Locus-specific microsatellite markers for the fungal chickpea pathogen Didymella rabiei (anamorph) Ascochyta rabiei.Molecular Ecology 9, 1939–1941.
Grewal, J.S. 1984 Evidence of physiological races in Ascochyta rabiei of chickpea. In Ascochyta blight and winter sowing of chickpea, eds. Saxena, M.C., Singh & K.B. pp. 55–65. Martinus Nijhoff/Dr. W. Junk Publisher, The Hague, The Netherlands.
Haberl, M. & Tautz, D. 1999 Comparative allele sizing can produce inaccurate allele size differences for microsatellites. Molecular Ecology 8, 1347–1350.
Hancock, J.M. 1996 Simple sequences in a minimal genome. Nature Genetics 14, 14–15.
Hantula, J., Niemi, E.M., Kaitera, J., Jalkanen, R. & Kurkela, T. 1998 Genetic variation of the resin top fungus in Finland as determined by random amplified microsatellites (RAMS). European Journal of Forest Pathology 28, 361–372.
Kaiser, W.J. 1995 World distribution of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea. Phytopathology 85, 1040 (Abstr.).
Kaiser, W.J. 1997 Inter-and intranational spread of ascochyta pathogens of chickpea, faba bean, and lentil. Canadian Journal of Plant Pathology 19, 215–224.
Kim, N.S., Park, N.I., Kim, S.H., Kim, S.T., Han, S.S. & Kang, K.Y. 2000 Isolation of TC/AG repeat microsatellite sequences for fingerprinting rice blast fungus and their possible horizontal transfer to plants Molecules and Cells 10, 127–134.
Longato, S. & Bonfante, P. 1997 Molecular identification of mycorrhizal fungi by direct amplification of microsatellite regions. Mycological Research 101, 425–432.
Moon, C.D., Tapper, B.A. & Scott, B. 1999 Identification of Epichloe¨ endophytes in planta by a microsatellite-based PCR fingerprinting assay with automated analysis. Applied and Environmental Microbiology 65, 1268–1279.
Morjane, H., Geistlinger, J., Harrabi, M., Weising, K. & Kahl, G. 1994 Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates form a single chickpea field in Tunisia. Current Genetics 26, 191–197.
Nene, Y.L. & Reddy, M.V. 1987 Chickpea diseases and their control. In The Chickpea, eds. Saxena, M.C. & Singh, K.B. pp. 233–270 C.A.B. International, Wallingford, Oxon, UK. ISBN 0-85198571-8.
Powell, W., Machray, G.C. & Provan, J. 1996 Polymorphism revealed by simple sequence repeats. Trends in Plant Sciences 1, 215–222.
Sambrook, J., Fritch, E.F. & Maniatis, T. 1989 Molecular Cloning A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbour Laboratory Press. ISBN 0–87969309-6.
Santra, D.K., Singh, G., Kaiser, W.J., Gupta, V.S., Ranjekar, P.K. & Muehlbauer, F.J. 2001 Molecular analysis of Ascochyta rabiei (Pass.) Labr., the pathogen of ascochyta blight in chickpea. Theoretical and Applied Genetics 102, 676–682.
Schaffer, H.E. & Sederoff, R.R. 1981 Improved estimation of DNA fragment lengths from agarose gels. Analytical Biochemistry 115, 113–122.
Schlo¨ tterer, 1998 Genome evolution: are microsatellites really simple sequences? Current Biology 8, 132–134.
Schwengel, D.A., Jedlicka, A.E., Nanthakumar, E.J., Weber, J.L. & Levitt, R.C. 1994 Comparison of fluorescence-based semi-automated genotyping of multiple microsatellite loci with autoradiographic techniques. Genomics 22, 46–54.
Singh, K.B., Hawtin, G.C., Nene, Y.L. & Reddy, M.V. 1981 Resistance in chickpeas to Ascochyta rabiei. Plant Disease 65, 586–587.
Sirjusingh, C. & Kohn, L.M. 2001 Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum Molecular Ecology Notes 1, 267–269.
Tautz, D. & Renz, M. 1984 Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Research 12, 4127–4138.
Tenzer, I., Ivanissevich, S., Morgante, M. & Gessler, C., 1999 Identification of microsatellite markers and their application to population genetics of Venturia inaequalis. Phytopathology 89, 748–753.
Trapero-Casas, A. & Kaiser, W.J. 1992 Development of Didymella rabiei, the teleomorph of Ascochyta rabiei on chickpea straw. Phytopathology 82, 1261–1266.
Turgeon, B.G. & Yoder, O.C. 2000 Proposed nomenclature for mating type genes of filamentous fungi. Fungal Genetics and Biology 31,1–5.
Udupa, S.M., Weigard, F., Saxena, M.C. & Kahl, G. 1998 Genotyping with RAPD and microsatellite markers resolves pathotype diversity in ascochyta blight pathogen in chickpea. Theoretical and Applied Genetics 97, 299–307.
Weber, J.L. 1990 Informativeness of human (dC-dA)n Æ (dG-dT)n polymorphisms. Genomics 7, 330–540.
Weising, K., Kaemmer, D., Epplen, J.T., Weigand, F., Saxena, M. & Kahl, G. 1991 DNA fingerprinting of Ascochyta rabiei with synthetic oligonucleotides Current Genetics 19, 483–489.
Weising, K., Nybom, H., Wolff, K. & Meyer, W. 1995 DNA fingerprinting in plants and fungi. Boca Raton, Florida: CRC press. ISBN 0–84938920-8.
Wilson, A.D. & Kaiser, W.J. 1995 Cytology and genetics of sexual incompatibility in Didymella rabiei. Mycologia 87, 795–805.
Zane, L., Bargelloni, L. & Patarnello, T. 2002 Strategies for microsatellite isolation: a review. Molecular Ecology 11, 1–16.
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Barve, M., Santra, D., Ranjekar, P. et al. Genetic diversity analysis of a world-wide collection of Ascochyta rabiei isolates using sequence tagged microsatellite markers. World Journal of Microbiology and Biotechnology 20, 735–741 (2004). https://doi.org/10.1007/s11274-004-1550-8
- Ascochyta blight
- Ascochyta rabiei
- chickpea pathogen
- STMS (sequence tagged microsatellite repeats)