Wetlands Ecology and Management

, Volume 16, Issue 6, pp 471–483 | Cite as

Status of arbuscular mycorrhizal fungi (AMF) in the Sundarbans of India in relation to tidal inundation and chemical properties of soil

  • Tanumi Kumar
  • Monoranjan Ghose
Original Paper


The arbuscular mycorrhizal status of fifteen mangroves and one mangrove associate was investigated from 27 sites of three inundation types namely, diurnal, usual springtide and summer springtide. Roots and rhizospheric soil samples were analysed for spore density, frequency of mycorrhizal colonization and some chemical characteristics of soil. Relative abundance, frequency and spore richness of AMF were assessed at each inundation type. All the plant species except Avicennia alba exhibited mycorrhizal colonization. The study demonstrated that mycorrhizal colonization and spore density were more influenced by host plant species than tidal inundation. Forty four AMF species belonging to six genera, namely Acaulospora, Entrophospora, Gigaspora, Glomus, Sclerocystis and Scutellospora, were recorded. Glomus mosseae exhibited highest frequency at all the inundation types; Glomus fistulosum, Sclerocystis coremioides and Glomus mosseae showed highest relative abundance at sites inundated by usual springtides, summer springtides and diurnal tides, respectively. Spore richness of AMF was of the order usual springtide > diurnal > summer springtide inundated sites. The mean spore richness was 3.27. Diurnally inundated sites had the lowest concentrations of salinity, available phosphorus, exchangeable potassium, sodium and magnesium. Statistical analyses indicated that mycorrhizal frequency and AMF spore richness were significantly negatively correlated to soil salinity. Spore richness was also significantly negatively correlated to available phosphorus. The soil parameters of the usual springtide inundated sites appeared to be favourable for the existence of maximum number of AMF. Glomus mosseae was the predominant species in terms of frequency in the soils of the Sundarbans.


Indian Sundarbans Mangroves Arbuscular mycorrhizal fungi Mycorrhizal colonization Tidal inundation Chemical soil parameters Relationships 



We are obliged to Dr. D. Roy of the Indian Statistical Institute, Kolkata for his statistical assistance. We are also thankful to the Conservator and Joint Director, Sundarbans Biosphere Reserve, and DFO, South 24-Parganas, West Bengal for the necessary permission they provided for the fieldwork. Last, but not the least, we thank the field assistants for their sincere help during root and soil collection.


  1. Abbott LK, Robson AD (1991) Factors influencing the occurrence of vesicular-arbuscular mycorrhizas. Agric Ecosyst Environ 35:121–150CrossRefGoogle Scholar
  2. Aboulkhair KS, El-Sokkary IH (1994) Effects of salinity, boron and sodium on the growth and root infection of VAM, Rhizobium and Frankia of seedlings of three tree species. J Agric Sci Egypt 19:2969–2980Google Scholar
  3. Aliasgharzadeh N, Rastin NS, Towfighi H, Alizadeh A (2001) Occurrence of arbuscular mycorrhizal fungi in saline soils of the Tabriz Plain of Iran in relation to some physical and chemical properties of soil. Mycorrhiza 11:119–122CrossRefGoogle Scholar
  4. Allen EB, Allen MF, Helm DJ, Trappe JM, Molina R, Rincon E (1995) Patterns and regulation of mycorrhizal plant and fungal diversity. Plant Soil 170:47–62CrossRefGoogle Scholar
  5. Anderson AJ (1992) The influence of the plant root in mycorrhizal formation: an integrative plant fungal process. Chapman and Hall, New York, pp 37–64Google Scholar
  6. Barrow JR, Havstad KM, McCaslin BD (1997) Fungal root endophytes in four-wing saltbush, Altiplex canescens, on arid rangeland of southwestern USA. Arid Soil Res Rehabil 11:177–185Google Scholar
  7. Baylis GTS (1975) The magnolioid mycorrhiza and mycotrophy in root systems derived from it. In: Sanders FE, Mosse B, Tinker PB (eds) Endomycorrhizas. Academic Press, London, pp 373–389Google Scholar
  8. Bhardwaj S, Dudeja SS, Khurana AL (1997) Distribution of VAM fungi in the natural ecosystem. Folia Microbiol 42:589–594CrossRefGoogle Scholar
  9. Carvalho LM, Caçador I, Martins-Loução MA (2001) Temporal and spatial variation of arbuscular mycorrhizas in salt marsh plants of the Tagus estuary (Portugal). Mycorrhiza 11:303–309CrossRefGoogle Scholar
  10. Cooke JC, Butler RH, Madole G (1993) Some observations on the vertical distribution of vesicular-arbuscular mycorrhizae in roots of salt marsh grasses growing in saturated soils. Mycologia 85:547–550CrossRefGoogle Scholar
  11. Daniels BA, Skipper HD (1982) Methods for the recovery and quantitative estimation of propagules from soil. In: Schenck NC (ed) Methods and principles of mycorrhizal research. American Phytopathological Society, St. Paul, pp 20–45Google Scholar
  12. Day LD, Sylvia DM, Collins ME (1987) Interaction among vesicular-arbuscular mycorrhizae, soil, and landscape position. Soil Sci Soc Am J 51:635–639CrossRefGoogle Scholar
  13. Eom AH, David C, Hartnett A, Gail WT, Wilson C (2000) Host plant species effects on arbuscular mycorrhizal fungal communities in tallgrass prairie. Oecologia 122:435–444CrossRefGoogle Scholar
  14. Forest Survey of India (1999) The state of forest report. Forest survey of India. Ministry of environment and Forests, Dehra DunGoogle Scholar
  15. Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal fungi isolated from soil by wet sieving and decanting. Trans Br Mycol Soc 46:235–244Google Scholar
  16. Ghose M (2001) Status of mangroves in Sundarbans of West Bengal, India. In: Bhat NR, Taha FK, Al-Nasser AY (eds) Mangrove ecosystems: natural distribution, biology and management. Proceedings of International Symposium on Mangrove Ecology and Biology. Kuwait Institute for Scientific Research, Kuwait, pp 7–16Google Scholar
  17. Gupta R, Krishnamurthy KV (1996) Response of mycorrhizal and non-mycorrhizal Arachis hypogaea to NaCl and acid stress. Mycorrhiza 6:145–149CrossRefGoogle Scholar
  18. Hayman DS (1982) Influence of soils and fertility on activity and survival of VAM fungi. Phytopathology 72:1119–1125Google Scholar
  19. Hildebrandt U, Janetta K, Ouzaid F, Renne B, Nawrath K, Bothe H (2001) Arbuscular mycorrhizal colonization of halophytes in central European salt marshes. Mycorrhiza 10:175–183CrossRefGoogle Scholar
  20. Hirrel MC (1981) The effect of sodium and chloride salts on the germination of Gigaspora margarita. Mycologia 73:610–617CrossRefGoogle Scholar
  21. Hirrel MC, Mehranaran H, Gerdemann JW (1978) Vesicular-arbuscular mycorrhiza in the Chenopodiaceae and Cruciferae: do they occur? Can J Bot 56:2813–2817CrossRefGoogle Scholar
  22. Hoefnagels MH, Broome SW, Shafer SR (1993) Vesicular-arbuscular myorrhizae in salt marshes in North Carolina. Estuaries 16:851–858CrossRefGoogle Scholar
  23. Jackson ML (1973) Soil chemical analysis. Prentice Hall of India Private Limited, New Delhi, IndiaGoogle Scholar
  24. Jindal V, Atval A, Sekhon BS, Singh R (1993) Effect of VAM on metabolism of mung plants under NaCl salinity. Plant Physiol Biochem 31:475–481Google Scholar
  25. Juniper S, Abbott L (1993) Vesicular arbuscular mycorrhizas and soil salinity. Mycorrhiza 4:45–57CrossRefGoogle Scholar
  26. Khan AG (1974) The occurrence of mycorrhizas in halophytes, hydrophytes and xerophytes, and Endogone spores in adjacent soils. J Gen Microbiol 81:7–14Google Scholar
  27. Khan AG (1993) Occurrence and importance of mycorrhizae in aquatic trees of New South Wales, Australia. Mycorrhiza 3:31–38CrossRefGoogle Scholar
  28. Kim CK, Weber DJ (1985) Distribution of VA mycorrhiza on halophytes on inland salt playas. Plant Soil 83:207–214CrossRefGoogle Scholar
  29. Kormanik PP, McGraw AC (1982) Quantification of vesicular-arbuscular mycorrhizae in plant roots. In: Schenck NC (ed) Methods and principles of mycorrhizal research. American Phytopathological Society, St. Paul, pp 20–45Google Scholar
  30. Koske RE (1987) Distribution of VAM fungi along a latitudinal temperature gradient. Mycologia 79:55–68CrossRefGoogle Scholar
  31. Landwehr M, Hildebrandt U, Wilde P, Nawrath K, Toth T, Biro B, Bothe H (2002) The arbuscular mycorrhizal fungus Glomus geosporum in European saline, sodic and gypsum soils. Mycorrhiza 12:199–211PubMedCrossRefGoogle Scholar
  32. Lee PJ, Koske RE (1994) Gigaspora gigantia: Seasonal, abundance and ageing of spores in a sand dune. Mycol Res 98:453–457CrossRefGoogle Scholar
  33. Lorgio EA, Julio RG, Peter LM (1999) Variation in soil microorganisms and nutrients underneath and outside the canopy of Adesmia bedwellii (Papilionaceae) shrubs in arid coastal Chile following drought and above average rainfall. J Arid Environ 42:61–70CrossRefGoogle Scholar
  34. Mankarios AT, Abdel-Fattah GM (1994) Ecology of VA mycorrhiza in some Egyptian soils. Egypt J Bot 34:135–152Google Scholar
  35. McGee PA (1989) Variation in propagule numbers of VAM fungi in a semi-arid soil. Mycol Res 92:28–33Google Scholar
  36. Mitsch WJ, Gooselink JG (1993) Mangrove wetlands. In: Mitsch WJ, Gooselink JG (eds) Wetlands. J.G. Van Nostrand Reinfold Publication, New York, pp 293–328Google Scholar
  37. Morton JB (1986) Three new species of Acaulospora (Endogonaceae) from high-aluminium, low pH soils in West Virginia. Mycologia 78:641–648CrossRefGoogle Scholar
  38. Mukerji KG, Manoharachary C, Chamola BP (eds) (2002) Techniques in mycorrhizal studies. Kluwer Academic Publishers, Dordrecht, Boston, LondonGoogle Scholar
  39. Muthukumar T, Udaiyan K (2000) Arbuscular mycorrhizas of plants growing in the Western Ghats region, Southern India. Mycorrhiza 9:297–313CrossRefGoogle Scholar
  40. Naskar KR, Guha Bakshi DN (1987) Mangrove swamps of the Sundarbans: an ecological perspective. Naya Prokash, Kolkata, IndiaGoogle Scholar
  41. Phillips JM, Hayman DS (1970) Improved procedure for clearing root and staining parasitic and VA-mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161CrossRefGoogle Scholar
  42. Pond EC, Menge JA, Jarrell WM (1984) Improved growth of tomato in salinized soil by VAM fungi collected from saline soils. Mycologia 76:74–84CrossRefGoogle Scholar
  43. Poss JA, Pond E, Menge JA, Jarrell WM (1985) Effect of salinity on mycorrhizal onion and tomato in soil with and without additional phosphorus. Plant Soil 88:307–319CrossRefGoogle Scholar
  44. Ragupathy S, Mahadevan A (1993) Distribution of vesicular-arbuscular mycorrhizae in plants and rhizosphere soils of the tropical plains, Tamil Nadu, India. Mycorrhiza 3:123–136CrossRefGoogle Scholar
  45. Reddy SR, Pindi PK, Reddy SM (2005) Molecular methods for research on arbuscular mycorrhizal fungi in India: problems and prospects. Curr Sci 89:1699–1709Google Scholar
  46. Redecker D, Hijri I, Wiemken A (2003) Molecular identification of arbuscular mycorrhizal fungi in root: Perspectives and problems. Folia Geobot 38:113–124CrossRefGoogle Scholar
  47. Rozema J, Arp W, Diggelen JV, Esbroek MV, Broekman R, Punte H (1986) Occurrence and ecological significance of vesicular-arbuscular mycorrhiza in the salt marsh environment. Acta Bot Neerl 35:457–467Google Scholar
  48. Schenck NC, Perez Y (eds) (1990) Manual for the identification of VA-mycorrhizal fungi. Synergistic, Gainesville, FlaGoogle Scholar
  49. Sengupta A, Chaudhuri S (1990) Vesicular arbuscular mycorrhiza (VAM) in pioneer salt marsh plants of the Ganges river delta in West Bengal (India). Plant Soil 122:111–113CrossRefGoogle Scholar
  50. Sengupta A, Chaudhuri S (1991) Ecology of heterotrophic dinitrogen fixation in the rhizosphere of mangrove plant community at the Ganges river estuary in India. Oecologia 87:560–564CrossRefGoogle Scholar
  51. Sengupta A, Chaudhuri S (1994) Atypical root endophytic fungi of mangrove plant community of Sundarban and their possible significance as mycorrhiza. J Mycopathol Res 32:29–39Google Scholar
  52. Sengupta A, Chaudhuri S (2002) Arbuscular mycorrhizal relations of mangrove plant community at the Ganges river estuary in India. Mycorrhiza 12:169–174PubMedCrossRefGoogle Scholar
  53. Stahl PD, Christensen M (1991) Population variation in the mycorrhizal fungus Glomus mosseae: breadth of environmental tolerance. Mycol Res 95:300–307Google Scholar
  54. Thapar HS, Khan SN (1985) Distribution of VA mycorrhizal fungi in forest soils of India. Indian J For 8:5–7Google Scholar
  55. Tomlinson PB (1986) The Botany of Mangroves. Paperback edn. Cambridge University Press, New YorkGoogle Scholar
  56. Tressner HD, Hayes JA (1971) Sodium chloride tolerance of terrestrial fungi. Appl Microbiol 22:210–213Google Scholar
  57. Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986) Mesure du taux de mycorhization VA d’un systéme radiculaire. Recherche de méthodes d’estimation ayant une signification fonctionnelle. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Physiological and genetical aspects of mycorrhizae. INRA Press, Paris, pp 217–221Google Scholar
  58. Udaiyan K, Karthikeyan A, Muthukumar T (1996) Influence of edaphic and climatic factors on dynamics of root colonization and spore density of vesicular-arbuscular mycorrhizal fungi in Acacia farnesiana Willd. and A. planifrons W. et. A. Mycorrhiza 11:65–71Google Scholar
  59. Walker C (1992) Systematics and taxonomy of the arbuscular endomycorrhizal fungi (Glomales)—a possible way forward. Agronomie 12:887–897CrossRefGoogle Scholar
  60. Walker C, Mize CW, McNabb HS (1982) Populations of endogonacae fungi at two locations in central Iowa. Can J Bot 60:2518–2529CrossRefGoogle Scholar
  61. Wang FY, Liu RJ, Lin XG, Zhou JM (2004) Arbuscular mycorrhizal status of wild plants in saline-alkaline soils of the Yellow River Delta. Mycorrhiza 14:133–137PubMedCrossRefGoogle Scholar
  62. Watson J (1928) Mangrove forests of the Malay Peninsula. Malayan Forest Records 6. Fraser & Neave Limited, SingaporeGoogle Scholar
  63. Yadav JSP, Bandopadhyay AK, Rao KVGK, Sinha TS, Biswas CR, Bandopadhyay BK, Dutta SK (1981) Management of coastal saline soils of Sundarbans. CSSRI-ICAR Bull 7:1–32Google Scholar

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© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Agricultural & Ecological Research UnitIndian Statistical InstituteKolkataIndia

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