Lectin-Modified Cryogels for Laccase Immobilization: a Decolorization Study

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In this presented work, poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogels were prepared by using the radicalic cryopolymerization technique, and then modified with lectin protein ConA. These ConA functionalized cryogels were used for the immobilization of laccase, and enzyme loading was found to be as 7.1 mg/g cryogel. Finally, these laccase immobilized cryogels were successfully used for the decolorization of six different types of dye molecules.

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  1. Bertrand, G. (1985). Sur la laccase et sur le pouvoir oxydant de cette diastase. Comptes Rendus. Académie des Sciences (Paris), 120, 266–269.

  2. Champagne, P. P., & Ramsay, J. A. (2010). Dye decolorization and detoxification by laccase immobilized on porous glass beads. Bioresource Technology, 101, 2230–2235.

  3. Claus, H., Faber, G., & König, H. (2002). Redox-mediated decolorization of synthetic dyes by fungal laccases. Applied Microbiology and Biotechnology, 59, 672–678.

  4. Dainiak, M. B., Galaev, I. Y., Kumar, A., Plieva, F. M., & Mattiasson, B. (2007). Chromatography of living cells using supermacroporous hydrogels, cryogels. In A. Kumar, I. Y. Galaev, & B. Mattiasson (Eds.), Cell separation. Advances in Biochemical Engineering/Biotechnology, vol 106. Berlin: Springer.

  5. Dogan, T., Bayram, E., Uzun, L., Şenel, S., & Denizli, A. (2015). Trametes versicolor laccase immobilized poly(glycidyl methacrylate) based cryogels for phenol degradation from aqueous media. Journal of Applied Polymer Science, 132, 41981.

  6. Duran, N., Rosa, M. A., D’Annibale, A., & Gianfreda, L. (2002). Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme and Microbial Technology, 31, 907–931.

  7. Erkurt, A. E., Ünyayar, A., & Kumbur, H. (2007). Decolorization of synthetic dyes by white rot fungi, involving laccase enzyme in the process. Process Biochemistry, 42, 1429–1435.

  8. Fernandez-Fernandez, M., Sanroman, M. A., & Moldes, D. (2013). Recent developments and applications of immobilized laccase. Biotechnology Advances, 31, 1808–1825.

  9. Giardina, P., Faraco, V., Pezzell, C., Piscitelli, A., Vanhulle, S., & Sannia, G. (2010). Laccases: a never-ending story. Cellular and Molecular Life Sciences, 67, 369–385.

  10. Kandelbauer, A., Maute, O., Kessler, R. W., Erlacher, A., & Gübitz, G. M. (2004). Study of dye decolorization in an immobilized laccase enzyme-reactor using online spectroscopy. Biotechnology and Bioengineering, 87, 552–563.

  11. Kiristi, M., Singh, V. V., de Ávila, B. E. F., Uygun, M., Soto, F., Uygun, D. A., et al. (2015). Lysozyme-based antibacterial nanomotors. ACS Nano, 9, 9252–9259.

  12. Kunamneni, A., Ghazi, I., Camarero, S., Ballesteros, A., Plou, F. J., & Alcalde, M. (2008). Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers. Process Biochemistry, 43, 169–178.

  13. Leonowicz, A., Cho, N. S., Luterek, J., Wilkolazka, A., Wojtas-Wasilewska, M., Matuszewska, A., et al. (2001). Fungal laccase: properties and activity on lignin. Journal of Basic Microbiology, 41, 185–227.

  14. Lin, S. H., & Peng, F. C. (1994). Treatment of textile wastewater by electrochemical method. Water Research, 28, 277–282.

  15. Lin, S. H., & Peng, F. C. (1996). Continuous treatment of textile wastewater by combined coagulation, electrochemical oxidation and activated sludge. Water Research, 30, 587–592.

  16. Liu, Y., Zeng, Z., Zeng, G., Tang, T., Pang, Y., Li, Z., et al. (2012). Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds. Bioresource Technology, 115, 21–26.

  17. Lozinsky, V. I., Galaev, I. Y., Plieva, F. M., Savina, I. N., Jungvid, H., & Mattiasson, B. (2003). Polymeric cryogels as promising materials of biotechnological interest. Trends in Biotechnology, 21, 445–451.

  18. Lu, L., Zhao, M., & Wang, Y. (2007). Immobilization of laccase by alginate–chitosan microcapsules and its use in dye decolorization. World Journal of Microbiology and Biotechnology, 23, 159–166.

  19. Marmion, D. M. (1991) Handbook of US colorants. Foods, drugs, cosmetics and medical devices. 3rd ed. Wiley, New York.

  20. Mayer, A. M., & Staples, R. C. (2002). Laccase: new functions for an old enzyme. Phytochemistry, 60, 551–565.

  21. Mechichi, T., Mhiri, N., & Sayadi, S. (2006). Remazol Brilliant Blue R decolourization by the laccase from Trametes trogii. Chemosphere, 64, 998–1005.

  22. Minussi, R. C., Pastore, G. M., & Duran, N. (2002). Potential applications of laccase in the food industry. Trends in Food Science & Technology, 13, 205–216.

  23. Moreira, M. T., Mielgo, I., Feijoo, G., & Lema, J. M. (2000). Evaluation of different fungal strains in the decolourisation of synthetic dyes. Biotechnology Letters, 22, 1499–1503.

  24. Murugesan, K., Dhamija, A., Nam, I. H., Kim, Y. M., & Chang, Y. S. (2007a). Decolourization of reactive black 5 by laccase: optimization by response surface methodology. Dyes and Pigments, 75, 176–184.

  25. Murugesan, K., Nam, I. H., Kim, Y. M., & Chang, Y. S. (2007b). Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum in solid state culture. Enzyme and Microbial Technology, 40, 1662–1672.

  26. Murugesan, K., Kim, Y. M., Jeon, J. R., & Chang, Y. S. (2009). Effect of metal ions on reactive dye decolorization by laccase from Ganoderma lucidum. Journal of Hazardous Materials, 168, 523–529.

  27. Nieto, M., Nardecchia, S., Peinado, C., Catalina, F., Abrusci, C., Gutiérrez, M. C., et al. (2010). Enzyme-induced graft polymerization for preparation of hydrogels: synergetic effect of laccase-immobilized-cryogels for pollutants adsorption. Soft Matter, 6, 3533–3540.

  28. Nishizawa, Y., Nakabayashi, K., & Shinagawa, E. (1995). Purification and characterization of laccase from white rot fungus Trametes sanguinea M85-2. Journal of Fermentation and Bioengineering, 80, 91–93.

  29. Peralta-Zamora, P., Pereira, C. M., Tiburtius, E. R. L., Moraes, S. G., Rosa, M.A., Minussi, R. C, et al. (2003). Decolorization of reactive dyes by immobilized laccase. Applied Catalysis B: Environmental, 42, 131–144.

  30. Ping, W., Xuerong, F. A., Li, C. U., Qiang, W. A., & Aihui, Z. H. (2008). Decolorization of reactive dyes by laccase immobilized in alginate/gelatin blend with PEG. Journal of Environmental Sciences, 20, 1519–1522.

  31. Silva, C., Silva, C. S., Zille, A., Guebitz, G. M., & Cavaco-Paulo, A. (2007). Laccase immobilization on enzymatically functionalized polyamide 6, 6 fibers. Enzyme and Microbial Technology, 41, 867–875.

  32. Soares, G. M. B., Costa-Ferreira, M., & de Amorim, M. T. P. (2001). Decolorization of an anthraquinone-type dye using a laccase formulation. Bioresearch Technology, 79, 171–177.

  33. Spinelli, D., Fatarella, E., Michele, A. D., & Pogni, R. (2013). Immobilization of fungal (Trametes versicolor) laccase onto Amberlite IR-120 H beads: optimization and characterization. Process Biochemistry, 48, 218–223.

  34. Stanescu, M. D., Fogorasi, M., Shaskolskiy, B. L., Gavrilas, S., & Lozinsky, V. I. (2010). New potential biocatalysts by laccase immobilization in PVA cryogel type carrier. Applied Biochemistry and Biotechnoogy, 160, 1947–1954.

  35. Stanescu, M. D., Sanislav, A., Ivanov, R. V., Hirtopeanu, A., & Lozinsky, V. I. (2011). Immobilized laccase on a new cryogel carrier and kinetics of two anthraquinone derivatives oxidation. Applied Biochemistry and Biotechnoogy, 165, 1789–1798.

  36. Stanescu, M. D., Gavrilas, S., Ludwig, R., Haltrich, D., & Lozinsky, V. I. (2012). Preparation of immobilized Trametes pubescens laccase on a cryogel-type polymeric carrier and application of the biocatalyst to apple juice phenolic compounds oxidation. European Food Research and Technology, 234, 655–662.

  37. Tavares, A. P. M., Silva, C. G., Drazic, G., Silva, A. M., Loureiro, J. M., & Faria, J. L. (2015). Laccase immobilization over multi-walled carbon nanotubes: kinetic, thermodynamic and stability studies. Journal of Colloid and Interface Science, 454, 52–60.

  38. Thurston, C. F. (1994). The structure and function of fungal laccases. Microbiology, 140, 19–26.

  39. Uygun, M. (2013). Preparation of laccase immobilized cryogels and usage for decolorization. Journal of Chemistry, 2013, 387181.

  40. Wesenberg, D., Kyriakides, I., & Agathos, S. N. (2003). White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnology Advances, 22, 161–187.

  41. Widsten, P., & Kandelbauer, A. (2008). Laccase applications in the forest products industry: a review. Enzyme and Microbial Technology, 42, 293–307.

  42. Willmott, N., Guthrie, J., & Nelson, G. (1998). The biotechnology approach to colour removal from textile effluent. Journal of the Society of Dyers and Colourists, 114, 38–41.

  43. Witayakran, S., & Ragauskas, A. J. (2009). Synthetic applications of laccase in green chemistry. Advanced Synthesis & Catalysis, 351, 1187–1209.

  44. Xu, R., Zhou, Q., Li, F., & Zhang, B. (2013). Laccase immobilization on chitosan/poly (vinyl alcohol) composite nanofibrous membranes for 2, 4-dichlorophenol removal. Chemical Engineering Journal, 222, 321–329.

  45. Yaropolov, A. I., Skorobogatko, O. V., Vartanov, S. S., & Varfolomeyev, S. D. (1994). Laccase. Applied Biochemistry and Biotechnology, 49, 257–280.

  46. Yinghui, D., Qiuling, W., & Shiyu, F. (2002). Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier. Letters in Applied Microbiology, 35, 451–456.

  47. Yoshida, H. (1883). Chemistry of laquer (urushi). Journal of the Chemical Society, 43, 472–486.

  48. Zhang, J., Xu, Z., Chen, H., & Zong, Y. (2009). Removal of 2, 4-dichlorophenol by chitosan-immobilized laccase from Coriolus versicolor. Biochemical Engineering Journal, 45, 54–59.

  49. Zollinger, H. (1987). Color chemistry-synthesis, properties and application for organic dyes and pigments. NewYork: VCH Publications.

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Bayraktaroğlu, M., Husein, İ., Aktaş Uygun, D. et al. Lectin-Modified Cryogels for Laccase Immobilization: a Decolorization Study. Water Air Soil Pollut 231, 31 (2020) doi:10.1007/s11270-020-4395-3

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  • Laccase
  • Decolorization
  • Immobilization
  • Cryogel
  • Concanavalin A