Tea Residue Boosts Dye Decolorization and Induces the Evolution of Bacterial Community
Considerable researches on removal of azo dyes have been reported in recent years, but few researchers have documented adsorption and/or transformation of anthraquinone dyes by physical, chemical, or biological treatment methods due to their fused aromatic structures. In this study, tea residue was found to have significant enhancement effect on the decolorization of anthraquinone dye reactive blue 19. This effect worked on different dye decolorizing bacterial florae and the natural bacterial flora from surface water and exhibited universal feature. Six single bacterial strains were isolated from bacterial flora DDMY2. Unexpectedly, all of them had poor decolorization capacity. High-throughput sequencing results revealed the community evolution of bacterial flora DDMY2 cultured with tea residue after 6 months and 12 months. It was found that the community structure changed dramatically because the influence of tea residue and the dominant functional genera, such as unclassified_o_Pseudomonadales, Stenotrophomonas, Bordetella, and Brevibacillus, was significantly enriched. Meanwhile, the evolved community structure could keep stable for a long time, resulting in the decolorization effect stabilized for a long time. This study provides the tea residue as the bioactivator that can be applied to boost the decolorization of dyes by various potential bacterial florae. It also enlarges our knowledge of making full use of biowaste in biological wastewater treatment.
KeywordsBioactivator Tea residue Decolorization Reactive blue 19 Community structure evolution
The authors acknowledge the financial support by the Fundamental Research Funds for the Central Universities (2232018G-11, 2232019D3-22), the National Key Research and Development Program of China (Grant No. 2016YFC0400501), the Graduate Student Innovation Fund of Donghua University (CUSF-DH-D-2019078), Anhui Provincial Natural Science Foundation (1808085QE176), the scientific research program of Anhui Provincial Education Department (KJ2018A0444) and the Suzhou University Startup Foundation for Doctor (2016jb04), the “Chenguang Program” supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission (No. 16CG40). This work was partially supported by Shanghai Leading Academic Discipline Project (B604).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Chen, C.-Y., Tsai, T.-H., Wu, P.-S., Tsao, S.-E., Huang, Y.-S., & Chung, Y.-C. (2018). Selection of electrogenic bacteria for microbial fuel cell in removing Victoria blue R from wastewater. Journal of Environmental Science and Health Part A-Toxic/Hazardous Substances & Environmental Engineering, 53, 108–115.Google Scholar
- Patil, C. S., Gunjal, D. B., Naik, V. M., Harale, N. S., Jagadale, S. D., Kadam, A. N., et al. (2019). Waste tea residue as a low cost adsorbent for removal of hydralazine hydrochloride pharmaceutical pollutant from aqueous media: An environmental remediation. Journal of Cleaner Production, 206, 407–418.CrossRefGoogle Scholar
- Sekuljica, N. Z., Prlainovic, N. Z., Stefanovic, A. B., Zuza, M. G., Cickaric, D. Z., Mijin, D. Z., et al. (2015). Decolorization of anthraquinonic dyes from textile effluent using horseradish peroxidase: optimization and kinetic study. The Scientific World Journal, 2015, 371625–371625.CrossRefGoogle Scholar
- Xie, X., Fu, J., Wang, H., & Liu, J. (2010). Heavy metal resistance by two bacteria strains isolated from a copper mine tailing in China. African Journal of Biotechnology, 9, 4056–4066.Google Scholar
- Yu, C., Xie, X., Zheng, X., Xu, L., Li, R., & Liu, J. (2016). Decolorization and repigmentation of reactive black 5 biodegradation and their mechanisms. Chemical Industry and Engineering Progress, 35, 2987–2996.Google Scholar
- Zhang, Q., Xie, X., Yu, C., Chen, Y., & Liu, J. (2017). Effects of different co-metabolic substrates on the decolorization of reactive black 5 by bacteria and the community structure of bacterial flora. Chinese Journal of Ecology, 36, 2572–2580.Google Scholar
- Zhou, J. Z., Bruns, M. A., & Tiedje, J. M. (1996). DNA recovery from soils of diverse composition. Applied and Environmental Microbiology, 62, 316–322.Google Scholar