Water, Air, & Soil Pollution

, 230:222 | Cite as

Nitrogen Biogeochemistry of Anaerobic Biodegradation of Naphthalene

  • Menghuan Zhang
  • Fengjun Zhang
  • Zhe Ma
  • Yuyu WanEmail author


A mixed community of bacteria was enriched from groundwater contaminated with naphthalene as the sole carbon source. Based on the results of 16S rRNA sequences, Acinetobacter and Pseudomonas were the predominant species in the naphthalene-enriched culture. Different initial forms of nitrogen, including nitrate, nitrite, and ammonium, were beneficial to naphthalene degradation, which was considered second-order kinetics and naphthalene could be decreased by 94.68% during the incubation period of 30 days with an initial naphthalene concentration of 0.5 mg/L. These clear biogeochemical denitrification signals, the consumption and accumulation of nitrate, nitrite, and ammonium during the incubation period, suggested that naphthalene degradation may be coupled with denitrification and DNRA metabolism. Nitrate and nitrite were reduced mainly as electron acceptors, and ammonium was utilized by microorganisms as an important inorganic nutrient for their growth and reproduction, which promoted the degradation of naphthalene. The results of this study contributed to the removal pathway and transformational mechanism of nitrogen and reveal their involvement in the anaerobic biodegradation of naphthalene.


Naphthalene Polycyclic aromatic hydrocarbons Nitrogen pollution Microbial degradation Groundwater 


Funding Information

This present work was financially supported by National Natural Science Foundation of China (Grant No. 41402207), and also supported by Science and Technology Project of Educational Department of Jilin Province for 13th Five Year Plan (JJKH20180159KJ).

Compliance with Ethical Standards

Conflict of Interests

The authors declare that they have no conflict of interests.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Menghuan Zhang
    • 1
  • Fengjun Zhang
    • 1
  • Zhe Ma
    • 1
    • 2
  • Yuyu Wan
    • 1
    • 2
    Email author
  1. 1.Key Lab of Groundwater Resources and Environment, Ministry of EducationJilin UniversityChangchunPeople’s Republic of China
  2. 2.Institute of Water Resources and EnvironmentJilin UniversityChangchunPeople’s Republic of China

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