Water, Air, & Soil Pollution

, 230:224 | Cite as

Bioethanol Mill Wastewater Purification by Combination of Coagulation-Flocculation and Microbial Treatment of Trametes versicolor INACC F200

  • Ajeng Arum SariEmail author
  • Tony Hadibarata
  • Ummu Hanifah
  • Ahmad Randy
  • Feni Amriani
  • Puspa Dewi N. Lotulung
  • Hasbi Yasin
  • Asep Saefumillah
  • Muhammad Ilyas


Black liquor is generated from the pretreatment process of biomass-based bioethanol production and due its environmental impact, should be treated effectively before discharged to the water body. Chemical treatment using coagulation-flocculation method was commonly used for wastewater treatment. In the case of black liquor, chemical treatment is often insufficient and further treatment was needed to degrade lignin in order to reduce its black coloration. This present study investigated the two-step treatment to decolorize black liquor using chemical coagulation-flocculation and biological treatment using white-rot fungus Trametes versicolor INACC F200. The biological treatment was optimized by applying a response surface methodology (RSM) of the utilization of CuSO4 concentration, Tween 80 concentration, and agitation. Furthermore, lignin degradation was also confirmed using FTIR and LC-MS. Initial chemical treatment using ferrous sulfate and polyacrylamide as coagulant-flocculant with a ratio of 3:3, resulted in black liquor decolorization at 80.9% and reduced the COD up to 90.77%. A full quadratic stepwise model was utilized with CuSO4 inducer, Tween 80 mediator, and agitation speed as the independent variables. Optimum decolorization of 96.188% was predicted when using 2 mM CuSO4, 2% Tween 80, and an agitation speed of 150 rpm. The highest enzyme activity during the decolorization process was lignin peroxidase (LiP). FT-IR and LC-MS profile showed that lignin-associated bond was eliminated and the molecular weight of lignin was decreased after the treatment. This study concludes the effective decolorization and delignification of black liquor by the two-step chemical and biological treatment.


Black liquor Coagulation Flocculation Response surface methodology Ligninolytic enzyme Trametes versicolor 



This research was supported by INSINAS – Ministry of Research, Technology and Higher Education, Indonesia, for the 2018 Fiscal Year. The part of this research was presented in International Conference on Engineering and Natural Science – Summer Session 2018 that funded by overseas conference grant (Bantuan Seminar Luar Negeri / BSLN) - Ministry of Research, Technology and Higher Education, Indonesia for the 2018 Fiscal Year.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Research Unit for Clean Technology, Indonesian Institute of SciencesJalan Cisitu SangkuriangBandungIndonesia
  2. 2.Department of Environmental Engineering, Faculty of Engineering and ScienceCurtin University MalaysiaMiriMalaysia
  3. 3.Department of Chemistry, Faculty of Science and MathematicsUniversitas IndonesiaDepokIndonesia
  4. 4.Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan Puspiptek SerpongUniversitas Diponegoro, TembalangTangerang SelatanIndonesia
  5. 5.Department of Statistic, Faculty of Science and MathematicsUniversitas DiponegoroTembalangIndonesia
  6. 6.Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Kawasan Cibinong Science CenterCibinongIndonesia

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