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Water, Air, & Soil Pollution

, 230:223 | Cite as

Seasonality of E. coli and Enterococci Concentrations in Creek Water, Sediment, and Periphyton

  • Matthew Daniel StockerEmail author
  • Jaclyn Elizabeth Smith
  • Cesar Hernandez
  • Dumitru Macarisin
  • Yakov Pachepsky
Article

Abstract

Environmental reservoirs of fecal indicator bacteria (FIB) are attracting increasing attention because of the ambiguity they present when assessing the microbial quality of water. FIB can survive and even grow in various environmental reservoirs which means FIB measured in the water column may not have originated directly from a fecal source. Sediment and periphyton, i.e., aquatic biofilms growing on submerged rocks, have been shown to harbor large populations of FIB in the environment. However, little is known about the spatial and temporal dynamics of FIB in periphyton. The objective of this work was to determine levels of the common FIB, Escherichia coli and enterococci, in creek water, sediment, and periphyton during the summer and winter. FIB were measured during two summer and winter sampling dates at five locations along a 2.8-km stretch of creek in Beltsville, Maryland. Significant differences in FIB by location were only observed for E. coli in water at one time point. Levels of FIB significantly declined from summer to winter in all media. FIB concentrations in periphyton ranged from 102 to 104 gdw−1 in the summer and from 100 to 104 CFU gdw−1 in the winter. When compared on a dry weight basis, periphyton contained higher concentrations of FIB than the sediment. Variability of FIB was in the order of water < sediment < periphyton. Levels of E. coli and enterococci measured in the same sample showed significant positive correlation in all media (rs = 0.87, 0.48, 0.70, for water, sediment, and periphyton, respectively). Results from this work show that fecal bacteria can persist in creek periphyton which may act as both a reservoir for fecal pathogens as well as a probable source of fecal bacteria to the water column.

Keywords

Fecal indicator bacteria FIB Periphyton Sediments Water quality Food safety 

Notes

Acknowledgements

The authors would like to acknowledge the help of the USDA’s Agricultural Research Learning Experience (ARLE) program along with the Hispanic Serving Institutions initiative for supporting researchers to help plan and conduct the work.

Funding Information

This work was supported through the USDA’s Agricultural Research Service project number 8042-12630-011-00D.

Supplementary material

11270_2019_4263_MOESM1_ESM.jpg (485 kb)
Supplemental Figure 1. Flow and precipitation data recorded during the observation period. S1, S2, W1, and W2 represent sampling dates of 7/21/2017, 7/27/2017, 1/31/2018, and 2/6/2018, respectively. Panels a and b represent summer and winter sampling periods, respectively. (JPG 485 kb)
11270_2019_4263_MOESM2_ESM.jpg (798 kb)
Supplemental Figure 2. Cumulative probability distributions of E. coli and enterococci concentrations along the stream reach for the four observation periods. E. coli concentrations in water, sediment, and periphyton are presented in panels a, b, and c while enterococci in the same media are presented in d, e and f. (JPG 797 kb)
11270_2019_4263_MOESM3_ESM.jpg (367 kb)
Supplemental Figure 3. Comparison of FIB concentrations in periphyton as reported using different units. White circles and triangles represent E. coli concentrations in CFU gdw-1 and CFU cm-2, respectively. Black circles and triangles represent enterococci concentrations in CFU gdw-1 and CFU cm-2, respectively. (JPG 366 kb)
11270_2019_4263_MOESM4_ESM.docx (70 kb)
ESM 4 (DOCX 69 kb)

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Copyright information

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Authors and Affiliations

  1. 1.Oak Ridge Institute for Science and EducationOak RidgeUSA
  2. 2.USDA-ARS Environmental Microbial and Food Safety LaboratoryBeltsvilleUSA
  3. 3.Department of Chemistry and BiochemistryUniversity of Texas at El PasoEl PasoUSA
  4. 4.Office of Regulatory Science, Center for Food Safety and Applied NutritionFood and Drug AdministrationCollege ParkUSA

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