Water, Air, & Soil Pollution

, 230:26 | Cite as

Immobilization of Heavy Metals in e-Waste Contaminated Soils by Combined Application of Biochar and Phosphate Fertilizer

  • Ling Huang
  • Chong Liu
  • Xiaowen Liu
  • Zhiliang ChenEmail author


This paper reports the effects of single and combined application of biochar and phosphate fertilizer on immobilization of heavy metals in e-waste-contaminated soils. The results showed that combined amending biochar and phosphate fertilizer improved physical and chemical characteristics of soil but resulted in ammonium nitrogen loss. Biochar combined with phosphate fertilizer increased shoot biomass of lettuce while biochar applied alone could inhibit the growth of lettuce. A distinct decrease of heavy metal concentrations in lettuce was observed in phosphate fertilizer + biochar (3.0%) treatments while highest heavy metal concentrations in shoots and roots were observed in control treatments. In phosphate fertilizer (0.8%) + biochar (3.0%) treatment, Cd, Cu, Pb, and Zn concentrations of lettuce leaf were reduced by 34.78%, 29.37%, 46.59%, and 40.95%, respectively. Biochar + phosphate fertilizer and biochar both reduced bioconcentration of Cd, Cu, Pb, and Zn in different tissues of lettuce while transshipment of Cd, Cu, Pb, and Zn from root to shoot increased after combined amendment of biochar with phosphate fertilizer. Application of phosphate fertilizer + biochar enhanced the immobilization of Cd, Cu, Pb, and Zn by decreasing the exchangeable Cd, Cu, Pb, and Zn in the soil. Precipitation, adsorption, ionic exchange, and chelation contributed to the good immobilization capacity of biochar + phosphate fertilizer on Cd, Cu, Pb, and Zn in e-waste-contaminated soils.


Biochar Phosphate fertilizer Amelioration Heavy metal Immobilization Lettuce 


Funding Information

This work was supported by the the National Key R&D Program of China (No.2017YFD0801300), Science and Technology Planning Project of Guangdong Province, China (No.2017B020203001), Fundamental Research Funds for the Central Public Welfare Research Institutes (No.PM-zx703-201803-079).


  1. Ahmad, M., Rajapaksha, A. U., Lim, J. E., Zhang, M., Bolan, N., Mohan, D., Vithanage, M., Lee, S. S., & Ok, Y. S. (2014). Biochar as a sorbent for contaminant management in soil and water: A review. Chemosphere, 99(3), 19–33.CrossRefGoogle Scholar
  2. Asai, H., Samson, B. K., Stephan, H. M., Songyikhangsuthor, K., Homma, K., Kiyono, Y., Inoue, Y., Shiraiwa, T., & Horie, T. (2009). Biochar amendment techniques for upland rice production in northern Laos: 1. Soil physical properties, leaf SPAD and grain yield. Field Crops Research, 111(s 1–2), 81–84.CrossRefGoogle Scholar
  3. Bolan, N., Kunhikrishnan, A., Thangarajan, R., Kumpiene, J., Park, J., Makino, T., Kirkham, M. B., & Scheckelh, K. (2014). Remediation of heavy metal(loid)s contaminated soils – To mobilize or to immobilize? Journal of Hazardous Materials, 266(4), 141–166.CrossRefGoogle Scholar
  4. Cao, X., Ma, L., Liang, Y., Gao, B., & Harris, W. (2011). Simultaneous immobilization of lead and atrazine in contaminated soils using dairy-manure biochar. Environmental Science & Technology, 45, 4884–4889.CrossRefGoogle Scholar
  5. Chen, Y., Chen, W., Lin, Y. C., Cheng, J. Z., & Pan, W. J. (2015). Effects of biochar on the micro-ecology of tobacco-planting soil and physiology of flue-cured tobacco. Chinese Journal of Applied Ecology, 26(12), 3781–3787 (In Chinese).Google Scholar
  6. Das, K. C., Singh, K., Adolphson, R., Hawkins, B., Oglesby, R., Lakly, D., & Day, D. (2010). Steam pyrolysis and catalytic steam reforming of biomass for hydrogen and biochar production. Applied Engineering in Agriculture, 26(1), 137–146.CrossRefGoogle Scholar
  7. Gai, X. P., Wang, H. Y., Liu, J., Zhai, L. M., Liu, S., Ren, T. Z., & Liu, H. B. (2014). Effects of feedstock and pyrolysis temperature on biochar adsorption of ammonium and nitrate. PLoS One, 9(12), e113888.CrossRefGoogle Scholar
  8. Hollister, C. C., Bisogni, J. J., & Lehmann, J. (2013). Ammonium, nitrate, and phosphate sorption to and solute leaching from biochars prepared from corn Stover (Zea mays L.) and oak wood (Quercus spp.). Journal of Environmental Quality, 42(1), 137–144.Google Scholar
  9. Jiang, J., Xu, R. K., Jiang, T. Y., & Li, Z. (2012). Immobilization of cu(II), Pb(II) and cd(II) by the addition of rice straw derived biochar to a simulated polluted Ultisol. Journal of Hazardous Materials., 229-230, 145–150.CrossRefGoogle Scholar
  10. Jin, H. P., Choppala, G. K., Bolan, N. S., Chung, J. W., & Chuasavathi, T. (2011). Biochar reduces the bioavailability and phytotoxicity of heavy metals. Plant and Soil, 348(1), 439–451.Google Scholar
  11. Khan, M. A., Kim, K. W., Wang, M. Z., & Lee, J. Y. (2008). Nutrient-impregnated charcoal: An environmentally friendly slow-release fertilizer. Environmentalist, 28(3), 231–235.CrossRefGoogle Scholar
  12. Kumpiene, J., Lagerkvist, A., & Maurice, C. (2008). Stabilization of As, Cr, Cu, Pb and Zn in soil using amendments – a review. Waste Management, 28(1), 215–225.Google Scholar
  13. Laird, D., Fleming, P., Wang, B. Q., Horton, R., & Karlen, D. (2010). Biochar impact on nutrient leaching from a Midwestern agricultural soil. Geoderma, 158(3), 436–442.CrossRefGoogle Scholar
  14. Lee, S. H., Park, H., Koo, N., Hyunc, S., & Hwangd, A. (2011). Evaluation of the effectiveness of various amendments on trace metals stabilization by chemical and biological methods. Journal of Hazardous Materials, 188(1–3), 44–51.CrossRefGoogle Scholar
  15. Lee, S. J., Lee, M. E., Chung, J. W., Park, J. H., Huh, K. Y., & Jun, G. I. (2013). Immobilization of Lead from Pb-contaminated soil amended with peat Moss. Journal of Chemistry, 2013, 509520.1–509520.6.Google Scholar
  16. Li, Q. S., Chen, Y., Fu, H. B., Cui, Z. H., Shi, L., Wang, L. L., & Liu, Z. F. (2012). Health risk of heavy metals in food crops grown on reclaimed tidal flat soil in the Pearl River estuary, China. Journal of Hazardous Materials, 227-228(16), 148–154.CrossRefGoogle Scholar
  17. Li, N., Kang, Y., Pan, W. J., Zeng, L. X., Zhang, Q. Y., & Luo, J. W. (2015). Concentration and transportation of heavy metals in vegetables and risk assessment of human exposure to bioaccessible heavy metals in soil near a waste-incinerator site, South China. Science of the Total Environment, 521–522, 144–151.CrossRefGoogle Scholar
  18. Li, C., Xiong, Y., Qu, Z., Xu, X., Huang, Q., & Huang, G. (2018). Impact of biochar addition on soil properties and water-fertilizer productivity of tomato in semi-arid region of Inner Mongolia, China. Geoderma, 331, 100–108.CrossRefGoogle Scholar
  19. Liu, Z. B., Ji, X. H., Peng, H., Tian, F. X., Wu, J. M., & Shi, L. H. (2012). Effects of phosphorous fertilizers on phytoavailability of cadmium in its contaminated soil and related mechanisms. Chinese Journal of Applied Ecology, 23(6), 1585–1590.Google Scholar
  20. Liu, J., He, X. X., Lin, X. R., Chen, W. C., Zhou, Q. X., Shu, W. S., & Huang, L. N. (2015). Ecological effects of combined pollution associated with e-waste recycling on the composition and diversity of soil microbial communities. Environmental Science & Technology, 49(11), 6438–6447.Google Scholar
  21. Lu, R. K. (2000). Soil and agro-chemistry analysis. Beijing: Chinese Agriculture Science and Technology Press (In Chinese).Google Scholar
  22. Luo, C., Liu, C., Wang, Y., Liu, X., Li, F., Zhang, G., & Li, X. (2011) Heavy metal contamination in soils and vegetables near an e-waste processing site, south China. Journal of Hazardous Materials, 186(1):481–490.Google Scholar
  23. Mackie, K. A., Marhan, S., Ditterich, F., Schmidt, H. P., & Kandeler, E. (2015). The effects of biochar and compost amendments on copper immobilization and soil microorganisms in a temperate vineyard. Agriculture, Ecosystems & Environment, 201, 58–69.CrossRefGoogle Scholar
  24. Pan, X. D., Wu, P. G., & Jiang, X. G. (2016). Levels and potential health risk of heavy metals in marketed vegetables in Zhejiang, China. Scientific Reports, 6, 20317.CrossRefGoogle Scholar
  25. Pandeya, S. B., Singh, A. K., & Jha, P. (1998). Labile pool of cadmium in sludge-treated soils. Plant and Soil, 203(1), 1–13.CrossRefGoogle Scholar
  26. Qiu, X., Fang, Z., Yan, X., Cheng, W., & Lin, K. (2013). Chemical stability and toxicity of nanoscale zero-valent iron in the remediation of chromium-contaminated watershed. Chemical Engineering Journal, 220(6), 61–66.CrossRefGoogle Scholar
  27. Rinklebe, J., Shaheen, S. M., & Frohne, T. (2016). Amendment of biochar reduces the release of toxic elements under dynamic redox conditions in a contaminated floodplain soil. Chemosphere, 142, 41–47.CrossRefGoogle Scholar
  28. Robson, T. C., Braungardt, C. B., Rieuwerts, J., & Worsfold, P. (2014). Cadmium contamination of agricultural soils and crops resulting from sphalerite weathering. Environmental Pollution, 184, 283–289.CrossRefGoogle Scholar
  29. Rodríguez-Vila, A., Asensio, V., Forjan, R., & Covelo, E. F. (2015). Chemical fractionation of cu, Ni, Pb and Zn in a mine soil amended with compost and biochar and vegetated with Brassica juncea L.. Journal of Geochemical Exploration, 158, 74–81.Google Scholar
  30. Rondon, M. A., Lehmann, J., Ramírez, J., & Hurtado, M. (2007). Biological nitrogen fixation by common beans (Phaseolus vulgaris L.) increases with bio-char additions. Biology & Fertility of Soils, 43(6), 699–708.CrossRefGoogle Scholar
  31. Sarkhot, D. V., Berhe, A. A., & Ghezzehei, T. A. (2013). Impact of biochar enriched with dairy manure effluent on carbon and nitrogen dynamics. Journal of Environmental Quality, 41(4), 1107–1114.CrossRefGoogle Scholar
  32. Scheifler, R., De, V. A., Coeurdassier, M., Crini, N., & Badot, P. M. (2006). Transfer of cd, cu, Ni, Pb, and Zn in a soil-plant-invertebrate food chain: A microcosm study. Environmental Toxicology & Chemistry, 25(3), 815–822.CrossRefGoogle Scholar
  33. Shah, T., Khan, S., Shah, P. D. Z., (2017). Soil Respiration, pH and EC as Influenced by Biochar. Soil and Environment, 36(01):77-83.Google Scholar
  34. Shen, Z., Som, A. M., Wang, F., Jin, F., McMillan, O., & Al-Tabbaa, A. (2016). Long-term impact of biochar on the immobilisation of nickel (II) and zinc (II) and the revegetation of a contaminated site. Science of the Total Environment, 542, 771–776.CrossRefGoogle Scholar
  35. Silber, A., Levkovitch, I., & Graber, E. R. (2010). pH-dependent mineral release and surface properties of cornstraw biochar: Agronomic implications. Environmental Science & Technology, 44(24), 9318–9323.CrossRefGoogle Scholar
  36. Song, Q., & Li, J. (2014). Environmental effects of heavy metals derived from the e-waste recycling activities in China: A systematic review. Waste Management, 34(12), 2587–2594.CrossRefGoogle Scholar
  37. Tang, X., Li, X., Liu, X., Hashmi, M. Z., Xu, J., & Brookes, P. C. (2015a). Effects of inorganic and organic amendments on the uptake of lead and trace elements by Brassica chinensis, grown in an acidic red soil. Chemosphere, 119, 177–183.CrossRefGoogle Scholar
  38. Tang, X., Pang, Y., Ji, P., Gao, P., Nguyen, T. H., & Tong, Y. (2015b). Cadmium uptake in above-ground parts of lettuce (Lactuca sativa L.). Ecotoxicology & Environmental Safety, 125, 102–106.CrossRefGoogle Scholar
  39. Tessier, A., Campbell, P. G. C., & Bisson, M. (1979). Sequential extraction procedure for the speciation of particulate trace metals. Analytical Chemistry, 51(7), 844–851.CrossRefGoogle Scholar
  40. Uchimiya, M., Bannon, D. I., Wartelle, L. H., Lima, I. M., & Klasson, K. T. (2012). Lead retention by broiler litter biochars in small arms range soil: Impact of pyrolysis temperature. Journal of Agricultural and Food Chemistry, 60(20), 5035–5044.Google Scholar
  41. Venegas, A., Rigol, A., & Vidal, M. (2015). Viability of organic wastes and biochars as amendments for the remediation of heavy metal-contaminated soils. Chemosphere, 119, 190–198.CrossRefGoogle Scholar
  42. Wang, S., Nan, Z., Prete, D., Ma, J., Liao, Q., & Zhang, Q. (2016). Accumulation, transfer, and potential sources of mercury in the soil-wheat system under field conditions over the loess plateau, Northwest China. Science of the Total Environment, 568, 245–252.CrossRefGoogle Scholar
  43. Wu, W., Dong, C., Wu, J., Liu, X., Wu, Y., Chen, X., & Yu, S. (2017). Ecological effects of soil properties and metal concentrations on the composition and diversity of microbial communities associated with land use patterns in an electronic waste recycling region. Science of the Total Environment, 601–602, 57–65.CrossRefGoogle Scholar
  44. Yang, F., Cao, X., Gao, B., Zhao, L., & Li, F. (2015). Short-term effects of rice straw biochar on sorption, emission, and transformation of soil NH4 +-N. Environmental Science & Pollution Research, 22(12), 9184–9192.CrossRefGoogle Scholar
  45. Yuan, X., Leng, L., Huang, H., Chen, X., Wang, H., Xiao, Z., Zhai, Y., Chen, H., & Zeng, G. (2015). Speciation and environmental risk assessment of heavy metal in bio-oil from liquefaction/pyrolysis of sewage sludge. Chemosphere, 120, 645–652.CrossRefGoogle Scholar
  46. Zhang, R. H., Li, Z. G., Liu, X. D., Wang, B. C., Zhou, G. L., Huang, X. X., Lin, C. F., Wang, A. H., & Brooks, M. (2017). Immobilization and bioavailability of heavy metals in greenhouse soils amended with rice straw-derived biochar. Ecological Engineering, 98, 183–188.CrossRefGoogle Scholar
  47. Zheng, R., Sun, G., Li, C., Reid, B. J., Xie, Z., Zhang, B., & Wang, Q. (2017). Mitigating cadmium accumulation in greenhouse lettuce production using biochar. Environmental Science & Pollution Research, 24(7), 6532–6542.Google Scholar
  48. Zorrig, W., Rouached, A., Shahzad, Z., Abdelly, C., Davidian, J. C., & Berthomieu, P. (2010). Identification of three relationships linking cadmium accumulation to cadmium tolerance and zinc and citrate accumulation in lettuce. Journal of Plant Physiology, 167(15), 1239–1247.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Ling Huang
    • 1
  • Chong Liu
    • 1
    • 2
  • Xiaowen Liu
    • 1
  • Zhiliang Chen
    • 1
    Email author
  1. 1.Ministry of Ecology and EnvironmentSouth China Institute of Environmental ScienceGuangzhouChina
  2. 2.School of Environmental Science and EngineeringSun Yat-sen UniversityGuangzhouChina

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