Simultaneous Determination of Selected Trace Contaminants in Drinking Water Using Solid-Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry
Accurate determination of emerging contaminants in drinking water constitutes a major environmental challenge for which highly sensitive analytical methods are needed. This work details the development of a novel highly sensitive solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) method for simultaneous determination of a diverse panel of widely used trace contaminants, including two pharmaceuticals (fluoxetine and gemfibrozil), three pesticides (3-hydroxycarbofuran, azinphos-methyl, and chlorpyrifos), and two hormones (testosterone and progesterone) in water. The method is highly reproducible and sensitive with detection limits at subnanogram per liter level (0.05–0.5 ng/L). It was used to monitor the occurrence of these contaminants in source and drinking water across 18 drinking water treatment facilities in Missouri, USA in 1 year including cold winter and hot summer seasons. The experiment results indicated that all of the monitored contaminant concentrations are very low, lower than or close to the method detection limits, in the selected water treatment facilities. Pesticide concentrations were slightly elevated in some source waters during hot season, whereas slightly higher pharmaceuticals were observed during cold season. The concentrations of two hormones were lower than the limits of detection in all the water samples. These contaminants were present, if any, at below detection limits in all treated drinking water samples analyzed, suggesting that treatment processes effectively removed the contaminants studied herein.
KeywordsSolid-phase extraction-HPLC-MS/MS Emerging water contaminants Seasonal change Pharmaceutical Pesticide Hormone
The authors would like to also thank Dr. Casey Burton and Charles Roberts for their editing assistance on this manuscript.
This study received funding from the Missouri Department of Natural Resources.
- de Alda, M. J. L., & Barcelo, D. (2001). Determination of steroid sex hormones and related synthetic compounds considered as endocrine disrupters in water by fully automated on-line solid-phase extraction-liquid chromatography-diode array detection. Journal of Chromatography A, 911(2), 203–210.CrossRefGoogle Scholar
- Appa, R., Mhaisalkar, V. A., Bafana, A., Devi, S. S., Krishnamurthi, K., Chakrabarti, T., & Naoghare, P. K. (2018). Simultaneous quantitative monitoring of four indicator contaminants of emerging concern (CEC) in different water sources of Central India using SPE/LC (ESI)MS-MS. Environmental Monitoring and Assessment, 190, 489.CrossRefGoogle Scholar
- Aznar, R., Albero, B., Sanchez-Brunete, C., Miguel, E., Martin-Girela, I., & Tadeo, J. L. (2017). Simultaneous determination of multiclass emerging contaminants in aquatic plants by ultrasound-assisted matrix solid-phase dispersion and GC-MS. Environmental Science and Pollution Research, 24(9), 7911–7920.CrossRefGoogle Scholar
- Baker, D. R., & Kasprzyk-Hordern, B. (2011). Multi-residue analysis of drugs of abuse in wastewater and surface water by solid-phase extraction and liquid chromatography-positive electrospray ionisation tandem mass spectrometry. Journal of Chromatography A, 1218(12), 1620–1631.CrossRefGoogle Scholar
- Ferrari, A., Venturino, A., & de D’Angelo, A. M. P. (2007). Muscular and brain cholinesterase sensitivities to azinphos methyl and carbaryl in the juvenile rainbow trout Oncorhynchus mykiss. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(3), 308–313.Google Scholar
- Kolpin, D. W., Furlong, E. T., Meyer, M. T., Thurman, E. M., Zaugg, S. D., Barber, L. B., & Buxton, H. T. (2002). Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-2000: a national reconnaissance. Environmental Science & Technology, 36(6), 1202–1211.CrossRefGoogle Scholar
- Mauriz, E., Calle, A., Lechuga, L. M., Quintana, J., Montoya, A., & Manclus, J. J. (2006). Real-time detection of chlorpyrifos at part per trillion levels in ground, surface and drinking water samples by a portable surface plasmon resonance immunosensor. Analytica Chimica Acta, 561(1–2), 40–47.CrossRefGoogle Scholar
- Méité, L., Soro, B. D., Aboua, N. K., Mambo, V., Traoré, K. S., Mazellier, P., De Laat, J. (2016). Qualitative determination of photodegradation products of progesterone and testosterone in aqueous solution. American Journal of Analytical Chemistry, 07(01), 22–33.Google Scholar
- Planas, C., Puig, A., Rivera, J., & Caixach, J. (2006). Analysis of pesticides and metabolites in Spanish surface waters by isotope dilution gas chromatography/mass spectrometry with previous automated solid-phase extraction—estimation of the uncertainty of the analytical results. Journal of Chromatography A, 1131(1–2), 242–252.CrossRefGoogle Scholar
- Schultz, M. M., Painter, M. M., Bartell, S. E., Logue, A., Furlong, E. T., Werner, S. L., & Schoenfuss, H. L. (2011). Selective uptake and biological consequences of environmentally relevant antidepressant pharmaceutical exposures on male fathead minnows. Aquatic Toxicology, 104(1–2), 38–47.CrossRefGoogle Scholar