Genotoxicity in the Offspring of Rats Exposed to Contaminated and Acidified Experimentally Soils
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The aim of this study was to evaluate the genotoxic and mutagenic potential of contaminated soil diluted in acidic solutions and not acidic, in the offspring of rats exposed during pregnancy and neonatal periods. To this end, a comet assay and micronucleus test were performed. Soil samples were solubilized in the following three solvents: distilled water (control group), acid solvent at pH 5.2, and acid solvent at pH 3.6. Soil and solvent were mixed in a rate of 1:2 in g/mL, and hydrofluoric acid was used in the acid solvents. In the comet assay, the tail length, percentage of DNA within the tail and tail moment was analyzed in the whole blood of the pups that were studied. The number of micronuclei found in the bone marrow cells was counted in the micronucleus test. In all parameters evaluated in the comet assay, the group exposed to the lowest pH value when associated with contaminated soil (p < 0.05) had the most damage. However, the micronucleus test showed differences between all exposed groups and the control group (p < 0.05). These results reaffirm the health risks related to the exposure to soil contaminants.
KeywordsEnvironmental exposure Reproduction Metals Comet assay Micronucleus test
- Da Silva-Júnior, F. M. R., Silva, P. F., Garcia, E. M., Klein, R. D., Peraza-Cardoso, G., Baisch, P. R., Vargas, V. M. F., & Muccillo-Baisch, A. L. (2013). Toxic effects of the ingestion of water-soluble elements found in soil under the atmospheric influence of an industrial complex. Environmental Geochemistry and Health, 35, 317–331.CrossRefGoogle Scholar
- Driscoll, C. T., Lawrence, G. B., Bulger, A. J., Butler, T. J., Cronan, C. S., Eagar, C., Lambert, K. F., Likens, G. E., Stoddard, J. L., & Weathers, K. C. (2001). Acidic deposition in the northeastern United States: sources and inputs, ecosystem effects, and management strategies. Bioscience, 51(3), 180–198.CrossRefGoogle Scholar
- EPA, U.S. Environmental Protection Agency (EPA) (2011). Exposure factors handbook: 2011 edition. National Center for Environmental Assessment, Washington, DC; EPA/600/R-09/052F. http://www.epa.gov/ncea/efh, 2011, Accessed 30 Nov 2014.
- Garcia, E. M., da Silva Junior, F. M. R., & Muccillo-Baisch, A. L. (2016). Mutagenic effect of contaminated soil on the offspring of exposed rats. Acta Scientiarum. Health Sciences, 38(1), 19–22.Google Scholar
- Glanz, J. T. (1995). Saving our soil: solutions for sustaining earth’s vital resource. USA: Joh. Bo.Google Scholar
- Lucio-Neto, M.P. (2011). Avaliação tóxica, citotóxica, genotóxica e mutagênica do composto 3-(2-Cloro-6-Fluorobenzil)-Imidazolidina-2,4-Diona em células eucarióticas. MSc Dissertation. Federal University of Piauí, Teresina.Google Scholar
- Muccillo-Baisch, A. L., Mirlean, N., Carrazzoni, D., Soares, M. C. F., Goulart, G. P., & Baisch, P. (2011). Health effects of ingestion of mercury-polluted urban soil: an animal experiment. Environmental Geochemistry and Health, 33, 1–11.Google Scholar
- Ribeiro, L. R., Salvadori, D. M. F., & Marques, E. K. (2003). Mutagênese ambiental. Canoas: Ulbra.Google Scholar
- Singh, H., et al. (1988). Isolation by screening of an expression library with a recognition site DNA. Cell Press, 52, 415–423.Google Scholar
- Zenick, H., & Clegg, E. D. (1989). Assessment of male reproductive toxicity. A risk assessment approach. In W. Hayes (Ed.), Principles and methods of Toxicology (pp. 275–309). New York: Raven Press.Google Scholar