Particle Size and Concentration Dependent Ecotoxicity of Nano- and Microscale TiO2 —Comparative Study by Different Aquatic Test Organisms of Different Trophic Levels
A comprehensive ecotoxicity assessment of three different nanosized TiO2 (with 16, 36 and 89 nm particle diameter) and one microscale TiO2 suspension (with 3264 nm particle diameter) was carried out with a special emphasis on the relation between product characteristics and toxic effect. The applied test battery included the combination of modified standardized tests (Aliivibrio fischeri bioluminescence inhibition test, Lemna minor growth inhibition test), and nonstandardized bioassays with unconventional physiological endpoints (Tetrahymena pyriformis phagocytic activity, the Daphnia magna heartbeat rate). Based on the lowest significant effect values, the tested aquatic organisms were the most sensitive to the microscale TiO2 suspension (with 3264 nm particle size). Although the three nanoscale TiO2 particles were aggregated in the A. fischeri and the L. minor growth media, significant inhibition rates were experienced at 0.1 and at 1 μg L─1 concentration of nTiO2 suspensions with 16 and 36 nm primary particle size, respectively. Larger aggregates may have also high impact on biological organisms. In case of the D. magna heartbeat rate test rapid agglomeration was avoided, but lower responses were found compared to other investigated systems. The short term T. pyriformis phagocytic activity test demonstrated outstanding sensitivity; three TiO2 suspensions were significantly toxic even at 0.1 μg L─1. The consequences of our study clearly indicated that nanoscale TiO2 may have an impact on the aquatic ecosystem which is strongly influenced by aggregation. The effect of exposure duration and concentration as contributing factors in nano-titanium dioxide mediated toxicity was also demonstrated.
KeywordsEcotoxicity TiO2 nanoparticles Particle size Bioluminescence Heartbeat rate Phagocytosis
The financial supports of the National Innovation Office (TECH_08-A4/2-2008-0161, CDFILTER project and TECH_09-A4-2009-0129, SOILUTIL project) and the New Hungary Development Plan (TÁMOP-4.2.1/B-09/1/KMR-2010-0002) are greatly acknowledged. We are grateful to Emese Vaszita for her contribution to language editing of the manuscript.
Supported through the New National Excellence Program of the Ministry of Human Capacities.
- Bondarenko, O. M., Heinlaan, M., Sihtmäe, M., Ivask, A., Kurvet, I., Joonas, E., Jemec, A., Mannerström, M., Heinonen, T., Rekulapelly, R., Singh, S., Zou, J., Pyykkö, I., Drobne, D., & Kahru, A. (2016). Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID. Nanotoxicology, 10(9), 1229–1242.CrossRefGoogle Scholar
- Bouwmeester, H., Poortman, J., Peters, R. J., Wijma, E., Kramer, E., Makama, S., Puspitaninganindita, K., Marvin, H. J., Peijnenburg, A. A., & Hendriksen, P. J. (2011). Characterization of translocation of silver nanoparticles and effects on whole-genome gene expression using an in vitro intestinal epithelium coculture model. ACS Nano, 5(5), 4091–4103.CrossRefGoogle Scholar
- Hasselöv, M., & Kaegi, R. (2009). Analysis and characterization of manufactured nanoparticles in aquatic environments. In J. Lead & E. Smith (Eds.), Environmental and human health impacts of nanotechnology. Chichester: John Wiley & Sons.Google Scholar
- Hund-Rinke, K., Baun, A., Cupi, D., Fernandes, T. F., Handy, R., Kinross, J. H., Navas, J. M., Peijnenburg, W., Schlich, K., Shaw, B. J., & Scott-Fordsmand, J. J. (2016). Regulatory ecotoxicity testing of nanomaterials—proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles. Nanotoxicology, 10(10), 1442–1447.CrossRefGoogle Scholar
- ISO. (2010). ISO 21338:2010(E). Water quality—kinetic determination of the inhibitory effects of sediment, other solids and coloured samples on the light emission of Vibrio fischeri (kinetic luminescent bacteria test). Geneva: International Organization for Standardization.Google Scholar
- von der Kammer, F., Ferguson, P. L., Holden, P. A., Masion, A., Rogers, K. R., Klaine, S. J., Koelmans, A. A., Horne, N., & Unrine, J. M. (2012). Analysis of engineered nanomaterials in complex matrices (environment and biota): general considerations and conceptual case studies. Environmental Toxicology and Chemistry, 31(1), 32–49.CrossRefGoogle Scholar
- Klaine, S. J., Alvarez, P. J., Batley, G. E., Fernandes, T. F., Handy, R. D., Lyon, D. Y., Mahendra, S., McLaughlin, M. J., & Lead, J. R. (2008). Nanomaterials in the environment: behavior, fate, bioavailability and effects. Environmental toxicology and chemistry, 27, 1825–1851.CrossRefGoogle Scholar
- Menard, A., Drobne, D., & Jemec, A. (2011). Ecotoxicity of nanosized TiO2. Review of in vivo data. Environ. Pollut., 159, 677–684.Google Scholar
- OECD 201. (2011). Freshwater algae and cyanobacteria, growth inhibition test. Paris: OECD Publisher.Google Scholar
- OECD 202. (2004). OECD guideline for testing chemicals. Daphnia magna acute immobilization test. Paris: OECD Publisher.Google Scholar
- Poda, A. R., Bednar, A. J., Harmon, A., Hull, M., Mitrano, D. M., Ranville, J. F., Steevens, J. (2011). Characterization of silver nanoparticles using flow-field flow fractionation interfaced to inductively coupled plasma mass spectrometry. Journal of Chromatography. A, 1218(27), 4219–4225.Google Scholar
- Schiess, N., Csaba, G., & Kőhidai, L. (2001). Chemotactic selection with insulin, di-iodotyrosine and histamine alters the phagocytotic responsiveness of Tetrahymena. Comp. Biochem. Phys. C, 128(4), 521–530.Google Scholar
- Ud-Daula, A. (2009). Catecholamine homeostasis in Tetrahymena species and high throughput toxicity testing of selected chemicals and ultrafine particles. [dissertation]. Technische Universität München.Google Scholar
- Uyar, A. E., Robichaud, C., Darby, M. R., Wiesner, M., & Zucker, L. G. (2008). Nano-titanium dioxide: risk assessment. Cambridge, Massachusetts: NBER Emerging Industries Conference.Google Scholar