Catalase and Phosphatase Activities During Hydrocarbon Removal from Oil-Contaminated Soil Amended with Agro-Industrial By-products and Macronutrients

  • María del Carmen Cuevas-Díaz
  • Ángeles Martínez-Toledo
  • Oswaldo Guzmán-López
  • Cinthya P. Torres-López
  • Areli del C. Ortega-Martínez
  • Lizbeth J. Hermida-Mendoza
Article
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Abstract

Microbiological activities are essential in the bioremediation of polluted soils. The enzymatic activities of microorganisms are usually used as a biological indicator of soil health. The aim of this work was to observe the catalase, acid phosphatase (AcP), and alkaline phosphatase (AlP) activities in soil that was amended with agro-industrial by-products and macronutrients during the process of total petroleum hydrocarbon (TPH) removal. To this end, microcosm tests were performed with soil and agro-industrial by-products ratios of 100:2:2, for soil:sugarcane bagasse pith:filter cake mud (SSF); 100:2, for both soil:sugarcane bagasse pith (SS); and for soil filter cake mud (SF). The macronutrients—carbon, nitrogen, and phosphorus—in the experimental treatments were adjusted to 100:10:1 with a solution of NH4NO3 and K2HPO4. The best TPH removal (51.4%) was obtained with SSF at 15 days. In addition, a significant correlation was observed between TPH removal and AlP as well as AcP (r = 0.74, p < 0.0001; r = 0.70, p < 0.0107, respectively). Fungi growth was also correlated with both AlP (r = 0.97, p < 0.0001) and AcP (r = 0.95, p < 0.0001) activities. Besides, bacterial and fungi growth showed a correlation with TPH (r = 0.86, p < 0.001; r = 0.77, p < 0.0034, respectively). It could be said that the agro-industrial by-products and macronutrients contributed to pollutant removal from the oil-polluted soil at relatively short amount of time. In addition, the enzymatic activities were increased after the treatment; in this study, the high sensitivity enzyme was AlP, and it could be used as an indirect indicator of oil pollutant removal.

Keywords

Biological indicator Bioremediation Filter cake mud Sugarcane bagasse pith Total petroleum hydrocarbons 
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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • María del Carmen Cuevas-Díaz
    • 1
    • 2
  • Ángeles Martínez-Toledo
    • 3
  • Oswaldo Guzmán-López
    • 1
  • Cinthya P. Torres-López
    • 1
  • Areli del C. Ortega-Martínez
    • 1
  • Lizbeth J. Hermida-Mendoza
    • 1
  1. 1.Faculty of Chemical SciencesUniversity of VeracruzCoatzacoalcosMexico
  2. 2.Institute of Biotechnology and Applied EcologyUniversity of VeracruzXalapaMexico
  3. 3.Faculty of EngineeringAutonomous University of San Luis PotosíSan Luis PotosíMexico

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