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Long-Term Operation of an ASBBR Used to Treat Dairy Effluent: Effect of the Recirculation Rate on System Monitoring, Kinetics, and Key Microorganisms

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Abstract

The aim of the present study was to evaluate the influence of the recirculation rate on the efficiency of a 1,000-L pilot anaerobic sequencing batch biofilm reactor (ASBBR) treating effluent from a small dairy plant over a long-term period (570 days). Three operational conditions were studied, in which recirculation rates were varied, resulting in upflow velocities of 0.2, 3.8, and 6.4 m h−1 and the cycle time of 48 h. The biomass was immobilized on plastic supports containing polyurethane foam. The organic loading rate varied according to the operations occurring in the dairy plant. After system stability had been verified, temporal profiles of the substrate and metabolite concentrations were obtained, allowing kinetic parameter inference. Sludge samples from the inoculum and from the reactor were analyzed through microscopic examination, molecular biology analyses, and specific methanogenic activity assays. The average efficiencies of organic matter removal were 82 ± 11, 84 ± 9, and 87 ± 9 % at velocities of 0.2, 3.8, and 6.4 m h−1, respectively. Microscopic examinations indicated that the fluorescent microorganisms decreased throughout the experiment, and they were not detected in the last condition. Homoacetogenesis was inferred as a possible pathway for H2 removal and for maintenance of the methanogenic process. Specific methanogenic activity increased throughout the monitoring period. It was possible to conclude that the ASBBR was efficient, robust, and reliable in treating dairy effluents under the conditions used.

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Acknowledgments

This study was supported by the Conselho nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasil, process number 482660/2007-4) and by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brasil, process numbers 2007/54589-4 and 2010/11972-5).

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Correspondence to Giovana Tommaso.

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Santana, R.S.S., Figueiredo, M.S., Pozzi, E.G. et al. Long-Term Operation of an ASBBR Used to Treat Dairy Effluent: Effect of the Recirculation Rate on System Monitoring, Kinetics, and Key Microorganisms. Water Air Soil Pollut 225, 2137 (2014). https://doi.org/10.1007/s11270-014-2137-0

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Keywords

  • Anaerobic reactor
  • ASBBR
  • Dairy effluents
  • Recirculation rate