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Potential Use of Newly Isolated Bacterial Strain Ochrobactrum anthropi in Bioremediation of Polychlorinated Biphenyls

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Abstract

The degradation ability of newly isolated bacterial strain Ochrobactrum anthropi toward polychlorinated biphenyls (PCBs) was examined under aerobic conditions. The strain was isolated from historically PCB-contaminated sediments from Strážsky canal in eastern Slovakia, surrounding of the former PCB producer. The degradation ability of the strain was enhanced by addition of other substrates and degradation inducers—biphenyl, glucose, both biphenyl and glucose, ivy leaves, and pine needles. The adaptation of cells membrane toward PCBs in the presence of abovementioned substrates was evaluated with the changes in fatty acid composition (membrane saturation, cistrans isomerization, and changes in branched fatty acids synthesis). The highest induction of PCB degradation and lowest cell adaptation in liquid medium was achieved using ivy leaves. On the other hand, lowest degradation was achieved when PCBs were added alone. Similar low degradation was observed in the presence of glucose addition together with biphenyl. Contrary, highest growth stimulation under the applied condition was observed. Obtained results indicated that addition of glucose together with biphenyl induced PCB degradation via bacterial growth stimulation, not via the induction of activity of degradation enzymes. Cut ivy leaves (containing terpenoic compounds serving as degradation inducer and structural analog of biphenyl) increased PCB removal from contaminated sediment by O. anthropi. Results indicate the degradation ability of O. anthropi toward penta-, hexa-, and hepta-chlorinated PCB congeners. The degradation of congeners with more than five chlorine atoms per molecule was detected in higher extent compared to dichlorinated congeners.

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Acknowledgment

The financial support from Slovak Grant Agency (grant No. 1/0734/12) and grant APVV-0656-12 from Ministry of Education, Science, and Sports are gratefully acknowledged. Authors are thankful to Branislav Vrana, PhD., from the National Reference Laboratory in Water Research Institute Bratislava for the critical comments.

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Correspondence to Slavomíra Murínová.

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Murínová, S., Dercová, K. Potential Use of Newly Isolated Bacterial Strain Ochrobactrum anthropi in Bioremediation of Polychlorinated Biphenyls. Water Air Soil Pollut 225, 1980 (2014). https://doi.org/10.1007/s11270-014-1980-3

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Keywords

  • Bioaugmentation
  • Biodegradation
  • Bioremediation
  • Membrane adaptation
  • Polychlorinated biphenyls