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Aromatic Hydrocarbon Degradation by Halophilic Archaea Isolated from Çamaltı Saltern, Turkey

  • Sevim Feyza Erdoğmuş
  • Burçin Mutlu
  • Safiye Elif Korcan
  • Kıymet Güven
  • Muhsin KonukEmail author
Article

Abstract

The aims of the present study were to identify the halophilic Archaea that can degrade aromatic hydrocarbons (namely, p-hydroxybenzoic acid, naphthalene, phenanthrene, and pyrene) and to determine their catabolic pathways in the process of degrading the hydrocarbons. It was determined nine archaeal isolates used p-hydroxybenzoic acid, naphthalene, phenanthrene, and pyrene as sole carbon and energy sources. The isolates were identified as Halobacterium piscisalsi, Halorubrum ezzemoulense, Halobacterium salinarium, Haloarcula hispanica, Haloferax sp., Halorubrum sp., and Haloarcula sp. by 16S rRNA gene sequences. Activity of catechol 1,2 dioxygenase and protocatechuate 3,4 dioxygenase enzyme of the ortho cleavage pathway were detected. Determination of the genes of these dioxygenases was also shown. This study clearly demonstrated for the first time that Halorubrum sp. and H. ezzemoulense among the isolates were able to grow at 20 % (w/v) NaCl, utilizing p-hydroxy-benzoic acid, naphthalene, phenanthrene, and pyrene as the sole carbon sources.

Keywords

Aromatic hydrocarbons Archaea Catechol 1, 2 dioxygenase Protocatechuate 3, 4 dioxygenase 

Notes

Acknowledgments

This study was partly supported by Project Number 071018 of the Anadolu University Research Foundation for the collection and identification of the isolates. Authors also wish to thank to Afyon Kocatepe University Scientific Research Committee for supporting this study financially (10. FENED.11). Our special thanks to Dr. H Shazly from Swansea-UK for editing the English of the paper.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Sevim Feyza Erdoğmuş
    • 1
  • Burçin Mutlu
    • 2
  • Safiye Elif Korcan
    • 1
  • Kıymet Güven
    • 2
  • Muhsin Konuk
    • 3
    Email author
  1. 1.Department of Biology, Faculty of Science and LiteraturesAfyon Kocatepe UniversityAfyonkarahisarTurkey
  2. 2.Department of Biology, Faculty of ScienceAnadolu UniversityEskişehirTurkey
  3. 3.Department of Molecular Biology and Genetics, Faculty of Engineering and Natural SciencesÜsküdar UniversityIstanbulTurkey

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