Water, Air, & Soil Pollution

, Volume 223, Issue 6, pp 3437–3444 | Cite as

Evaluation of the Influence of Multiple Environmental Factors on the Biodegradation of Dibenzofuran, Phenanthrene, and Pyrene by a Bacterial Consortium Using an Orthogonal Experimental Design

  • Raquel Simarro
  • Natalia González
  • L. Fernando Bautista
  • M. Carmen Molina
  • Emanuele Schiavi


For a bioremediation process to be effective, we suggest to perform preliminary studies in laboratory to describe and characterize physicochemical and biological parameters (type and concentration of nutrients, type and number of microorganisms, temperature) of the environment concerned. We consider that these studies should be done by taking into account the simultaneous interaction between different factors. By knowing the response capacity to pollutants, it is possible to select and modify the right treatment conditions to enhance bioremediation.


PAH degradation Orthogonal Microbial consortium Optimization 



This work has been funded by the Spanish Ministry of Environment (1.1-373/2005/3-B and 01/2006/2-1.1) and by Fundación Alfonso Martín Escudero. The consortium C2PL05 was isolated from soil samples kindly provided by Repsol S.A. This work is framed within the Official Máster en Ciencia y Tecnología Ambiental of the Universidad Rey Juan Carlos.

The authors have declared no conflict of interest.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Raquel Simarro
    • 1
  • Natalia González
    • 1
  • L. Fernando Bautista
    • 2
  • M. Carmen Molina
    • 1
  • Emanuele Schiavi
    • 3
  1. 1.Department of Biology and Geology, ESCETUniversidad Rey Juan CarlosMadridSpain
  2. 2.Department of Chemical and Environmental Technology, ESCETUniversidad Rey Juan CarlosMadridSpain
  3. 3.Department of Applied Mathematics, ESCETUniversidad Rey Juan CarlosMadridSpain

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