Water, Air, & Soil Pollution

, Volume 223, Issue 3, pp 1045–1054 | Cite as

Decolorization of Azo, Triphenylmethane and Anthraquinone Dyes by Laccase of a Newly Isolated Armillaria sp. F022

  • Tony Hadibarata
  • Abdull Rahim Mohd Yusoff
  • Azmi Aris
  • Salmiati
  • Topik Hidayat
  • Risky Ayu Kristanti
Article

Abstract

A newly isolated white-rot fungus, Armillaria sp. strain F022, was isolated from the decayed wood in a tropical rain forest. Strain F022 was capable of decolorizing a variety of synthetic dyes, including azo, triphenylmethane, and anthraquinone dyes, with an optimal efficiency of decolorization obtained when dyes added after 96 h of culture, with the exception of Brilliant Green. All of the tested dyes were decolorized by the purified laccase in the absence of any redox mediators, but only a few were completely removed, while others were not completely removed even when decolorization time was increased. The laccase, with possible contributions from unknown enzymes, played a role in the decolorization process carried out by Armillaria sp. F022 cultures, and this biosorption contributed a negligible part to the decolorization by cultures. The effect of dye to fungal growth was also investigated. When dyes were added at 0 h of culture, the maximum dry mycelium weight (DMW) values in the medium containing Brilliant Green were 1/6 of that achieved by the control group. For other dyes, the DMW was similar with control. The toxic tolerance of dye for the cell beads was excellent at least up to a concentration of 500 mg/l. The optimum conditions for decolorization of three synthetic dyes are at pH 4 and 40°C.

Keywords

Armillaria sp. F022 Brilliant Green Laccase activity Microbial decolorization Reactive Black 5 Remazol Brilliant Blue R 

Notes

Acknowledgement

A part of this research was financially supported by Research University Grant from the Universiti Teknologi Malaysia (Vote No. 00J31), which is gratefully acknowledged.

References

  1. Asgher, M., Shah, S. A. H., Ali, M., & Legge, R. L. (2006). Decolorization of some reactive dyes by white rot fungi isolated in Pakistan. World Journal Microbiology & Biotechnology, 22, 89–93.CrossRefGoogle Scholar
  2. Benito, G. G., Miranda, M. P., & De Los Santos, D. R. (1997). Decolorization of wastewater from an alcoholic fermentation process with Trametes versicolor. Bioresource Technology, 61, 33–37.CrossRefGoogle Scholar
  3. Carliell, C. M., Barclay, S. J., Naidoo, N., Buckley, C. A., Mullholland, D. A., & Senior, E. (1995). Microbial decolourisation of a reactive azo dye under anaerobic conditions. Water SA, 21, 61–69.Google Scholar
  4. Claus, H. (2003). Laccases and their occurrence in prokaryotes. Archives of Microbiology, 179, 145–150.Google Scholar
  5. Couto, R. S., & Herrera, J. T. (2006). Industrial biotechnological applications of laccases: A review. Biotechnology Advance, 24, 500–513.CrossRefGoogle Scholar
  6. Eggert, C., Lafayette, P. R., Temp, U., Eriksson, K. E. L., & Dean, J. F. D. (1998). Molecular analysis of a laccase gene from the white-rot fungus Pycnoporus cinnabarinus. Applied and Environmental Microbiology, 64, 1766–1772.Google Scholar
  7. Ferreira, V. S., Magalhaes, D. B., Kling, S. H., Da Silva, J. G., & Bon, E. P. S. (2000). N-Demethylation of methylene blue by lignin peroxidases from Phanerochaete chrysosporium. Applied Biochemistry and Biotechnology, 84, 255–265.CrossRefGoogle Scholar
  8. Fu, Y., & Viraraghavan, T. (2001). Fungal decolorization of dye wastewaters: A review. Bioresource Technology, 9, 251–262.CrossRefGoogle Scholar
  9. Ghodake, G. S., Kalme, S. D., Jadhav, J. P., & Govindwar, S. P. (2008). Purification and partial characterization of lignin peroxidase from Acinetobacter calcoaceticus NCIM 2890 and its application in decolorization of textile dyes. Applied Biochemistry and Biotechnology, 152, 6–14.CrossRefGoogle Scholar
  10. Hamedaani, H. R., Sakurai, A., & Sakakibara, M. (2007). Decolorization of synthetic dyes by a new manganese peroxidase producing white rot fungus. Dyes and Pigments, 72, 157–162.CrossRefGoogle Scholar
  11. Hatvani, N., & Mecs, I. (2001). Production of laccase and manganese peroxidase by Lentinus edodes on malt containing by product of the brewing process. Process Biochemistry, 37, 491–496.CrossRefGoogle Scholar
  12. Kahraman, S. S., & Gurdal, I. H. (2002). Effect of synthetic and natural culture media on laccase production by white-rot fungi. Bioresource Technology, 82, 215–217.CrossRefGoogle Scholar
  13. Laessoe, T. G. (2002). Mushrooms. London: Dorling Kindersley Publishers.Google Scholar
  14. Lopez, C., Mielgo, I., Moreira, M. T., Feijoo, G., & Lema, J. M. (2002). Enzymatic membrane reactors for biodegradation of recalcitrant compounds. Journal of Biotechnology, 99, 249–257.CrossRefGoogle Scholar
  15. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.Google Scholar
  16. Mou, D. G., Lim, K. K., & Shen, H. P. (1991). Microbial agents for decolorization of dye wastewater. Biotechnology Advance, 9, 613–622.CrossRefGoogle Scholar
  17. Nyanghong, G. S., Gomesa, J., Gubitzc, G. M., Zvauyab, R., Readd, J., & Steinera, W. (2002). Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Resources, 36, 1449–1456.Google Scholar
  18. Pace, G. (1998). Mushrooms of the world: With 20 photographs and 634 full color illustrations of species and varieties. New York: Firefly Books Ltd.Google Scholar
  19. Paszczynski, A., Pasti-Grigsby, M. B., Goszczynski, S., Crawford, R. L., & Crawford, D. L. (1992). Mineralization of sulfonated azo dyes and sulfanilic acid by Phanerochaete chrysosporium and Streptomyces chromofuscus. Applied and Environmental Microbiology, 58, 3598–3604.Google Scholar
  20. Sayan, E. (2006). Optimization and modeling decolorization and COD reduction of reactive dye solution by ultrasound-assisted adsorbtion. Chemical Engineering, 119, 175–181.CrossRefGoogle Scholar
  21. Spadaro, J. T., Gold, M. H., & Ranganathan, V. (1992). Degradation of azo dyes by the lignin-degrading fungus Phanerochaete chrysosporium. Applied and Environmental Microbiology, 58, 2397–2401.Google Scholar
  22. Tavaker, M., Svobodova, K., Kuplenk, J., Novotny, C., & Pavko, C. (2006). Biodegradation of Azo Dye RO16 in different reactors by immobilized Irpex lacteus. Acta Chimica Slovenica, 53, 338–343.Google Scholar
  23. Thurston, C. F. (1994). The structure and function of fungal laccases. Microbiology, 140, 19–26.CrossRefGoogle Scholar
  24. Verma, P., & Madamwar, D. (2003). Decolorization of synthetic dyes by a newly isolated strain of Serratia marcescens. World Journal of Microbiology & Biotechnology, 19, 615–618.CrossRefGoogle Scholar
  25. Wong, Y., & Yu, J. (1999). Laccase catalysed decolorization of synthetic dyes. Water Resources, 33, 3512–3520.Google Scholar
  26. Young, L., & Yu, J. (1997). Ligninase-catalized decolorization of synthetic dyes. Water Resources, 31, 1187–1193.Google Scholar
  27. Zouari-Mechichi, H., Mechichi, T., Dhoui, A., Sayad, S., Martínez, A. T., & Martínez, M. J. (2006). Laccase purification and characterization from Trametes trogii isolated in Tunisia: Decolorization of textile dyes by the purified enzyme. Enzyme and Microbial Technology, 39, 141–148.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Tony Hadibarata
    • 1
  • Abdull Rahim Mohd Yusoff
    • 1
  • Azmi Aris
    • 1
  • Salmiati
    • 1
  • Topik Hidayat
    • 2
    • 3
  • Risky Ayu Kristanti
    • 4
  1. 1.Institute of Environmental and Water Research ManagementUniversiti Teknologi MalaysiaSkudaiMalaysia
  2. 2.Department of Biological Science, Faculty of Bioscience and BioengineeringUniversiti Teknologi MalaysiaSkudaiMalaysia
  3. 3.Department of Biology Education, Faculty of Mathematic and Natural ScienceUniversity of Education (UPI) BandungBandungIndonesia
  4. 4.Department of ResearchInterdisciplinary Graduate School of Medicine and Engineering, University of YamanashiKofuJapan

Personalised recommendations