Water, Air, & Soil Pollution

, Volume 223, Issue 3, pp 1045–1054 | Cite as

Decolorization of Azo, Triphenylmethane and Anthraquinone Dyes by Laccase of a Newly Isolated Armillaria sp. F022

  • Tony Hadibarata
  • Abdull Rahim Mohd Yusoff
  • Azmi Aris
  • Salmiati
  • Topik Hidayat
  • Risky Ayu Kristanti


A newly isolated white-rot fungus, Armillaria sp. strain F022, was isolated from the decayed wood in a tropical rain forest. Strain F022 was capable of decolorizing a variety of synthetic dyes, including azo, triphenylmethane, and anthraquinone dyes, with an optimal efficiency of decolorization obtained when dyes added after 96 h of culture, with the exception of Brilliant Green. All of the tested dyes were decolorized by the purified laccase in the absence of any redox mediators, but only a few were completely removed, while others were not completely removed even when decolorization time was increased. The laccase, with possible contributions from unknown enzymes, played a role in the decolorization process carried out by Armillaria sp. F022 cultures, and this biosorption contributed a negligible part to the decolorization by cultures. The effect of dye to fungal growth was also investigated. When dyes were added at 0 h of culture, the maximum dry mycelium weight (DMW) values in the medium containing Brilliant Green were 1/6 of that achieved by the control group. For other dyes, the DMW was similar with control. The toxic tolerance of dye for the cell beads was excellent at least up to a concentration of 500 mg/l. The optimum conditions for decolorization of three synthetic dyes are at pH 4 and 40°C.


Armillaria sp. F022 Brilliant Green Laccase activity Microbial decolorization Reactive Black 5 Remazol Brilliant Blue R 



A part of this research was financially supported by Research University Grant from the Universiti Teknologi Malaysia (Vote No. 00J31), which is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Tony Hadibarata
    • 1
  • Abdull Rahim Mohd Yusoff
    • 1
  • Azmi Aris
    • 1
  • Salmiati
    • 1
  • Topik Hidayat
    • 2
    • 3
  • Risky Ayu Kristanti
    • 4
  1. 1.Institute of Environmental and Water Research ManagementUniversiti Teknologi MalaysiaSkudaiMalaysia
  2. 2.Department of Biological Science, Faculty of Bioscience and BioengineeringUniversiti Teknologi MalaysiaSkudaiMalaysia
  3. 3.Department of Biology Education, Faculty of Mathematic and Natural ScienceUniversity of Education (UPI) BandungBandungIndonesia
  4. 4.Department of ResearchInterdisciplinary Graduate School of Medicine and Engineering, University of YamanashiKofuJapan

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