Comparison of the Transport of Tetracycline-Resistant and Tetracycline-Susceptible Escherichia coli Isolated from Lake Michigan
- 194 Downloads
It was recently reported that tetracycline could enhance the mobility of manure-derived Escherichia coli within saturated porous media (Walczak et al. (Water Research 45:1681–1690, 2011)). It was also shown, however, that E. coli from various sources could display marked variation in their mobility (Bolster et al. (Journal of Environmental Quality 35:1018–1025, 2009)). The focus of this research was to examine if the observed difference in the mobility of manure-derived tetracycline-resistant (tetR) and tetracycline-susceptible (tetS) E. coli strains was source-dependent. Specifically, E. coli were isolated from Lake Michigan, and the influence of tetracycline resistance on Lake Michigan-derived E. coli was investigated through column transport experiments. Additionally, a variety of cell morphology and surface properties were determined and related to the observed bacterial transport behavior. Our experimental results showed that, consistent with previous observations, the deposition rate coefficients of the tetR E. coli strain was ∼20–100% higher than those of the tetS E. coli strain. The zeta potential of the tetR E. coli cells was ∼25 mV more negative than the tetS E. coli cells. Because the surfaces of the E. coli cells and the quartz sands were negatively charged, the repulsive electrostatic double-layer interaction between the tetR E. coli cells and the quartz sands was stronger, and the mobility of the tetR E. coli cells in the sand packs was thus higher. The tetR E. coli cells were also more hydrophilic than the tetS E. coli cells. Results from migration to hydrocarbon phase (MATH) tests showed that about ∼35% more tetS E. coli cells partitioned to the hydrocarbon phase. As it was previously shown that cell hydrophobicity could enhance the attachment of bacterial cells to quartz sand, the difference in cell hydrophobicity could also have contributed to the observed higher mobility of the tetR E. coli cells. The size of the tetR and tetS E. coli cells were similar, suggesting that the observed difference in their mobility was not size-related. Characterization of cell surface properties also showed that tetR and tetS E. coli cells differed slightly in cell-bound lipopolysaccharide contents and had distinct outer membrane protein profiles. Such difference could alter cell surface properties which in turn led to changes in cell mobility.
KeywordsAntibiotic-resistant bacteria Escherichia coli Bacteria transport Groundwater contamination
SX was supported by University of Wisconsin-Milwaukee and University of Wisconsin Groundwater Research Program (WR10R007). SLB is the recipient of an NIH grant (5R00GM083147-04). We thank Dr. Douglas A. Steeber, Dr. Heather A. Owen, Thomas Schuck, and Steven E. Hardcastle for their assistance.
- Cleasby, J. L., Logsdon, G. S. (1999). Granular bed and percoat filtration. In: R. D. Letterman (Ed.), Water quality and treatment: A handbook of community water supplies, 5th ed (pp. 8.1–8.99). New York, McGraw-Hill, Inc.Google Scholar
- Clinical and Laboratory Standards Institute (2006). Performance standards for antimicrobial disk susceptibility tests; approved standard-ninth edition. Wayne PA.Google Scholar
- Elimelech, M., Gregory, J., Jia, X., & Williams, R. A. (1998). Particle deposition and aggregation measurement, modelling, and simulation. Oxford: Butterworth-Heinemann.Google Scholar
- Faguy, D. M., Bayley, D. P., Kostyukova, A. S., Thomas, N. A., & Jarrell, K. F. (1996). Isolation and characterization of flagella and flagellin proteins from the thermoacidophilic archaea Thermoplasma volcanium and Sulfolobus shibatae. Journal of Bacteriology, 178, 902–905.Google Scholar
- Freeze, R. A., & Cherry, J. A. (1979). Groundwater. Englewood Cliffs: Prentice-Hall.Google Scholar
- Halbert, L. W., Kaneene, J. B., Ruegg, P. L., Warnick, L. D., Wells, S. J., Mansfield, L. S., et al. (2006). Evaluation of antimicrobial susceptibility patterns in Campylobacter spp isolated from dairy cattle and farms managed organically and conventionally in the midwestern and northeastern United States. Journal of the American Veterinary Medical Association, 228, 1074–1081.CrossRefGoogle Scholar
- Hutson, S. S., Barber, N. L., Kenny, J. F., Linsey, K. S., Lumia, D. S., & Maupin, M. A. (2004). Estimated use of water in the United States in 2000, U.S. Geological Survey Circular 1268 (p. 46). Reston: US Geological Survey.Google Scholar
- Kenny, J. F., Barber, N. L., Hutson, S. S., Linsey, K. S., Lovelace, J. K., Maupin, M. A. (2009). Estimated use of water in the United States in 2005.Google Scholar
- Luczkiewicz, A., Jankowska, K., Fudala-Ksiazek, S., Olanczuk-Neyman, K. (2010). Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant. Water research (in press)Google Scholar
- Parveen, S., Lukasik, J., Scott, T. M., Tamplin, M. L., Portier, K. M., Sheperd, S., et al. (2006). Geographical variation in antibiotic resistance profiles of Escherichia coli isolated from swine, poultry, beef and dairy cattle farm water retention ponds in Florida. Journal of Applied Microbiology, 100, 50–57.CrossRefGoogle Scholar
- Pembrey, R. S., Marshall, K. C., & Schneider, R. P. (1999). Cell surface analysis techniques: what do cell preparation protocols do to cell surface properties? Applied and Environmental Microbiology, 65, 2877–2894.Google Scholar
- Salmore, A. K., Hollis, E. J., & McLellan, S. L. (2006). Delineation of a chemical and biological signature for stormwater pollution in an urban river. J Wat. Health, 4, 247–262.Google Scholar
- US EPA (2000). Improved enumeration methods for the recreational water quality indicators: Enterococci and Escherichia coli, http://www.epa.gov/nerlcwww/RecManv.pdf, p. 52.
- Varga, C., Rajic, A., McFall, M. E., Reid-Smith, R. J., Deckert, A. E., Pearl, D. L., et al. (2008). Comparison of antimicrobial resistance in generic Escherichia coli and Salmonella spp. cultured from identical fecal samples in finishing swine. Canadian Journal of Veterinary Research-Revue Canadienne De Recherche Veterinaire, 72, 181–187.Google Scholar
- Weight, W. D. (2008). Hydrogeology field manual (2nd ed.). New York: McGraw-Hill.Google Scholar
- World Health Organization (2003). 1st Joint FAO/OIE/WHO expert workshop on non-human antimicrobial usage and antimicrobial resistance: Scientific assessment, http://www.who.int/foodsafety/publications/micro/en/amr.pdf, Geneva.