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Water, Air, and Soil Pollution

, Volume 207, Issue 1–4, pp 5–18 | Cite as

Remediation of Metal-Contaminated Soil by Organic Metabolites from Fungi II—Metal Redistribution

  • Zandra ArwidssonEmail author
  • Bert Allard
Article

Abstract

Exudation of low molecular weight organic acids by fungi was studied in a project focusing on bioremediation of metal-contaminated soils. The production of acids (mainly oxalic and citric acid) as a response to nutrient variations and presence of metals has recently been reported (Arwidsson et al. 2009). A significant release of metals was observed and was related not only to the production of organic acids but also to the resulting pH decrease in the systems. The processes governing the release and redistribution of metals in the soil–water fungus system were the focus of the present continuation of the project, based on observations of Aspergillus niger, Penicillium bilaiae, and a Penicillium sp. The release of lead was 12% from the soil with the second highest initial load (1,600 mg kg−1), while the release of copper was 90% from the same soil (140 mg kg−1). The dominating mechanism behind the release and subsequent redistribution was the change in pH, going from near neutral to values in the range 2.1–5.9, reflecting the production of organic acids. For some of the systems, the formation of soluble complexes is indicated (copper, at intermediate pH) which favors the metal release. Iron is assumed to play a key role since the amount of secondary iron in the soils is higher than the total load of secondary heavy metals. It can be assumed that most of the heavy metals are initially associated with iron-rich phases through adsorption or coprecipitation. These phases can be dissolved, or associated metals can be desorbed, by a decrease in pH. It would be feasible to further develop a process in technical scale for remediation of metal-contaminated soil, based on microbial metabolite production leading to formation of soluble metal complexes, notably with copper.

Keywords

Bioremediation Fungi Metals Oxalic acid Citric acid 

Notes

Acknowledgment

Financial support was obtained from the Foundation for Knowledge and Competence Development, as well as Sakab-Kumla Environmental Foundation. The classification of the fungi was made by P. Fransson, at the Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, which is gratefully acknowledged. The valuable help from T. von Kronhelm (Remediation Technologies, SAKAB AB), P. van Hees, E. Johansson, and S. Karlsson (Department of Natural Science, Orebro University) is most thankfully accredited.

References

  1. Allard, B. (1995). Groundwater. In B. Salbu & E. Steines (Eds.), Trace elements in natural waters (pp. 151–176). Boca Raton: CRC.Google Scholar
  2. Arwidsson, Z., Johansson, E., von Kronhelm T., Allard B., van Hees P. (2009). Remediation of metal contaminated soil by organic metabolites from fungi I—production of organic acids. Water, Air, and Soil pollution. doi: 10.10007/s11270-009-0067-z.
  3. Bowen, H. J. M. (1979). Environmental chemistry of the elements. New York: Academic.Google Scholar
  4. Chen, M., Ma, L. Q., Singh, S. P., Cao, R. X., & Melamed, R. (2003). Field demonstration of in situ immobilization of soil Pb using P amendments. Advances in Environmental Research, 8, 93–102.CrossRefGoogle Scholar
  5. Dahlén, J., Hagberg, J., & Karlsson, S. (2000). Analysis of LMWOAs in water with capillary zone electrophoresis employing indirect photometric detection. Fresenius Journal of Analytic Chemistry, 336, 488–493.Google Scholar
  6. Gadd, G. M. (2007). Geomycology: biogeochemical transformations of rocks, minerals, metals, and radionuclides by fungi, bioweathering and bioremediation. Mycological Research, 111, 3–49.CrossRefGoogle Scholar
  7. Greman, H., Velikonja-Bolta, S., Vodnik, D., Kos, B., & Lestan, D. (2001). EDTA enhanced heavy metal phytoextraction: metal accumulation, leaching and toxicity. Plant and Soil, 235, 105–114.CrossRefGoogle Scholar
  8. Huang, D.-L., Zeng, G.-M., Jiang, X.-Y., Feng, C.-L., Yu, H.-Y., Huang, G.-H., et al. (2006). Bioremediation of Pb-contaminated soil by incubating with Phanerochaete chrysporium and straw. Journal of Hazardous Materials, B134, 268–276.CrossRefGoogle Scholar
  9. Jorgensen, K. S., Puustinen, J., & Suortti, A.-M. (2000). Bioremediation of petroleum hydrocarbon-contaminated soil by composting in biopiles. Environmental Pollution, 107, 245–254.CrossRefGoogle Scholar
  10. Krantz-Rülcker, C., Allard, B., & Ephraim, J. H. (1994). Acid–base properties of a soil fungus, Tricoderma harzianum. Environmental Science and Technology, 28, 1502–1505.CrossRefGoogle Scholar
  11. Laine, M. M., & Jorgensen, K. S. (1997). Effective and safe composting of chlorophenol-contaminated soil in pilot scale. Environmental Science and Technology, 31(2), 371–378.CrossRefGoogle Scholar
  12. Karaffa, L., & Kubicek, C. (2003). Aspergillus niger citric acid accumulation: do we understand this well working black-box? Applied Microbiology and Biotechnology, 61, 189–196.Google Scholar
  13. Ledin, M. (2000). Accumulation of metals by microorganisms—processes and importance for soil systems. Earth Science Reviews, 51, 1–31.CrossRefGoogle Scholar
  14. Lindsay, W. L., & Norvell, W. A. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science of Society American Journal, 42, 421–428.CrossRefGoogle Scholar
  15. Mulligan, C. N., Kamali, M., & Gibbs, B. F. (2004). Bioleaching of heavy metals from a low-grade mining ore using Aspergillus niger. Journal of Hazardous Materials, 110, 77–84.CrossRefGoogle Scholar
  16. Ousmanova, D., & Parker, W. (2007). Fungal generation of organic acids for removal of lead from contaminated soil. Water, Air, and Soil Pollution, 179, 365–380.CrossRefGoogle Scholar
  17. Peters, W. R. (1999). Chelant extraction of heavy metals from contaminated soils. Journal of Hazardous Materials, 66, 151–210.CrossRefGoogle Scholar
  18. Riffaldi, R., Levi-Minzi, R., Cardelli, R., Palumbo, S., & Saviozzi, A. (2006). Soil biological activities in monitoring the bioremediation of diesel oil-contaminated soil. Water, Air, and Soil Pollution, 170, 3–15.CrossRefGoogle Scholar
  19. Ruby, M. V., Davis, A., & Nicholson, A. (1994). In situ formation of lead phosphates in soils as a method to immobilize lead. Environmental Science and Technology, 28, 646–654.CrossRefGoogle Scholar
  20. Sayer, J. A., Cotter-Howells, J. D., Watson, C., Hillier, S., & Gadd, G. M. (1999). Lead mineral transformation by fungi. Current Biology, 9, 691–694.CrossRefGoogle Scholar
  21. SEPA. (2007). Lägesbeskrivning av efterbehandlingsarbetet i landet 2006, dnr 642-737-07 Rf. Stockholm: SEPA.Google Scholar
  22. Shacklette, H.T., and Boerugen, J.G. (1984). Element concentrations in soils and other surficial materials of the conterminous United States, USGS Prof. Paper 1270, U.S. Government Printing.Google Scholar
  23. Stanforth, R., & Qiu, J. (2001). Effect of phosphate treatment on the solubility of lead in contaminated soil. Environmental Geology, 41, 1–10.CrossRefGoogle Scholar
  24. Tessier, A., Campell, P. G. C., & Bisson, M. (1979). Sequential extraction procedure for the speciation of particulate trace metals. Analytical Chemistry, 51(7), 844–851.CrossRefGoogle Scholar
  25. White, C., Sayer, J. A., & Gadd, G. M. (1997). Microbial solubilization and immobilization of toxic metals: key biogeochemical processes for treatment of contamination. FEMS Microbial reviews, 20, 503–516.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Man–Technology–Environment Research Centre, School of Science and TechnologyÖrebro UniversityÖrebroSweden
  2. 2.SAKAB ABKumlaSweden

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