Water, Air, and Soil Pollution

, Volume 206, Issue 1–4, pp 385–394

Effect of Biosolids and Cd/Pb Interaction on the Growth and Cd Accumulation of Brassica rapa Grown in Cd-Contaminated Soils

  • Hsuen-Li Chen
  • Hung-Yu Lai
  • Su-Mei Wang
  • Yu-Chen Kuo
  • Chih-Jen Lu


Mixed metals in the cropped lands in central Taiwan contaminated about 230 ha. According to the Soil and Groundwater Protection Remediation Act (SGWR Act) of Taiwan, these lands were restored. However, some grains of paddy rice grown in these remediated soils still contained more than 0.5 mg Cd kg−1, which the Department of Health of Taiwan notified as the maximum allowable Cd content in rice. The suitability of planting edible crops in these soils is now in doubt. Brassica rapa is the crop most often sold in Taiwan's market and is planted in the interval between the first and second stages of planting of paddy rice, especially in central Taiwan where this experiment was conducted. A pot experiment was conducted using soils contaminated artificially with Cd or both Cd and Pb. The soil was then amended with 5% of biosolid and followed by planting of B. rapa. The objectives were to study the effect of biosolid amendment on the soil and the interaction between Cd and Pb on the growth of and Cd accumulation in B. rapa. Experimental result showed that the biomass and the accumulation of Cd by B. rapa were significantly increased in the biosolid-amended soils compared with the control. Lead has a synergistic effect on enhancing the accumulation of Cd by B. rapa grown in artificially Cd-contaminated soils.


Biosolid Brassica rapa Cadmium (Cd) Interaction Lead (Pb) 


  1. Adriano, D. C. (1986). Cadmium. In D. C. Adriano (Ed.), Trace elements in the terrestrial environment (pp. 106–155). New York, NY: Springer.Google Scholar
  2. Alloway, B. J. (1995). Heavy metals in soils. Glasgow, UK: Blackie Academic & Professional.Google Scholar
  3. Bidwell, A. M., & Dowdy, R. H. (1987). Cadmium and zinc availability to corn following termination of sewage sludge applications. Journal of Environmental Quality, 16, 438–442.Google Scholar
  4. Blaylock, M. J., Salt, D. E., Dushenkov, S., Zakharova, O., Gussman, C., Kapulnik, Y., et al. (1997). Enhanced accumulation of Pb in Indian mustard by soil-applied chelating agents. Environment Science and Technology, 31, 860–865.CrossRefGoogle Scholar
  5. Brallier, S., Harrison, R. B., Henry, C. L., & Dongsen, X. (1996). Liming effects on availability of Cd, Cu, Ni, and Zn in a soil amended with sewage sludge 16 years previously. Water, Air and Soil Pollution, 86, 195–206.CrossRefGoogle Scholar
  6. Carlson, R. W., & Bazzaz, F. A. (1977). Growth reduction in American sycamore (Plantanus occidentalis L.) caused by Pb–Cd interaction. Environmental Pollution, 12, 243–253.CrossRefGoogle Scholar
  7. Carlson, R. W., & Rolfe, G. L. (1979). Growth of ryegrass and fescue as affected by lead–cadmium–fertilizer interaction. Journal of Environmental Quality, 8, 348–352.Google Scholar
  8. Corey, R. B., King, L. D., Hing, C. L., Fanning, D. S., Street, J. J., & Walker, J. M. (1987). Effects of sludge properties on accumulation of trace elements by crops. In A. L. Page, et al. (Eds.), Land application of sludge (pp. 25–51). Chelsea, MI: Lewis.Google Scholar
  9. EPA/Taiwan. (2002). The digestion methods of heavy metal in soils by aqua regia, method code no: NIEA S321.62C. Taipei, Taiwan: Environmental Protection Administration of Taiwan ROC.Google Scholar
  10. Epstein, E. (2003). Land application of sewage sludge and biosolids. Boca Raton, FL: CRC.Google Scholar
  11. Gardiner, D. T., Miller, R. W., Badamchian, B., Azzari, A. S., & Sisson, D. R. (1995). Effects of repeated sewage sludge application on plant accumulation of heavy metal. Agriculture and Ecosystem Environment, 55, 1–6.CrossRefGoogle Scholar
  12. Gardner, W. H. (1986). Water content. In A. Klute, et al. (Eds.), Methods of soil analysis. Part 1. Physical and mineralogical method (2nd ed., pp. 493–544). Madison, WI: Agronomy Monograph 9.Google Scholar
  13. Gee, G. W., & Bauder, J. W. (1986). Particle-size analysis. In A. Klute, et al. (Eds.), Methods of soil analysis. Part 1. Physical and mineralogical method (2nd ed., pp. 383–412). Madison, WI: Agronomy Monograph 9.Google Scholar
  14. Harmon, M. E., & Lajtha, K. (1999). Analysis of detritus and organic horizons for mineral and organic constitutes. In G. P. Robertson, D. C. Coleman, C. S. Bledsoe & P. Sollins (Eds.), Standard soil methods for long-term ecological research (pp. 143–165). New York, NY: Oxford University Press.Google Scholar
  15. Hassett, J. J., Miller, J. E., & Koeppe, D. E. (1976). Interaction of lead and cadmium on maize root growth and uptake of lead and cadmium by roots. Environmental Pollution, 11, 297–302.CrossRefGoogle Scholar
  16. Jarvis, S. C., Jones, H. P., & Hopper, M. J. (1976). Cadmium uptake from solution by plants and its transport from roots to shoots. Plant and Soil, 44, 179–191.CrossRefGoogle Scholar
  17. Jing, J., & Logan, T. J. (1992). Effects of sewage sludge cadmium concentration on chemical extractability and plant uptake. Journal of Environmental Quality, 21, 73–81.Google Scholar
  18. Joint FAO/WHO Food Standards Programme (2002). Distribution of the Report of the Thirty-Fourth Session of the Codex Committee on Food Additives and Contaminants (Alinorm 03/12). Rome.Google Scholar
  19. Kabata-Pendias, A., & Pendias, H. (2001). Trace elements in soils and plants. Boca Raton, FL: CRC.Google Scholar
  20. Lai, H. Y., & Chen, Z. S. (2006). The influence of EDTA application on the interactions of cadmium, zinc, and lead and their uptake of rainbow pink (Dianthus chinensis). Journal of Hazardous Materials, 137, 1710–1718.CrossRefGoogle Scholar
  21. Lai, H. Y., Su, S. W., Lin, C. C., & Chen, Z. S. (2007). A study on the recovery of soil fertility of two metal-contaminated soils after treating with soil acid washing. Journal of Science and Technology (National Yunlin University of Science and Technology), 16, 39–46.Google Scholar
  22. Lock, K., & Janssen, C. R. (2003). Influence of ageing on zinc bioavailability in soils. Environmental Pollution, 126, 371–374.CrossRefGoogle Scholar
  23. McBride, M. B. (1995). Toxic metal accumulation from agricultural use of sludge: are USEPA regulations protective? Journal of Environmental Quality, 24, 5–18.CrossRefGoogle Scholar
  24. McKenna, I. M., Chaney, R. L., & Williams, F. M. (1993). The effects of cadmium and zinc interactions on the accumulation and tissue distribution of zinc and cadmium in lettuce and spinach. Environmental Pollution, 79, 113–120.CrossRefGoogle Scholar
  25. McLean, E. O. (1982). Soil pH and lime requirement. In A. L. Page, et al. (Eds.), Methods of soil analysis. Part 2. Chemical and microbiological properties (2nd ed., pp. 199–224). Madison, WI: Agronomy Monograph 9.Google Scholar
  26. Naidu, R., Kookana, R. S., Sumner, M. E., Harter, R. D., & Tiller, K. G. (1997). Cadmium sorption and transport in variable charge soils: a review. Journal of Environmental Quality, 26, 602–607.Google Scholar
  27. Nelson, D. W., & Sommers, L. E. (1982). Total carbon, organic carbon, and organic matter. In A. L. Page, et al. (Eds.), Methods of soil analysis. Part 2. Chemical and microbiological properties (2nd ed., pp. 539–580). Madison, WI: Agronomy Monograph 9.Google Scholar
  28. Nogales, R., Gallardo-Lara, F., Benitez, E., Soto, J., Hervas, D., & Polo, A. (1997). Metal extractability and availability in a soil after heavy application of either nickel or lead in different forms. Water, Air and Soil Pollution, 94, 33–44.Google Scholar
  29. NRC (National Research Council). (1996). Use of reclaimed water and sludge in food crop production. Washington, DC: National Academies.Google Scholar
  30. Oberle, S. L., & Keeney, D. R. (1994). Interactions of sewage sludge with soil–crop–water systems. In C. E. Clapp, W. E. Larson & R. H. Dowdy (Eds.), Sewage sludge: land utilization and the environment. Madison, WI: ASA, CSSA, and SSSA.Google Scholar
  31. Peterson, A. E., Speth, P. E., Corvey, R. B., Wright, T. H., & Schlecht, P. L. (1994). Effect of twelve years of liquid digested sludge application on the soil phosphorus level. In C. E. Clapp, et al. (Eds.), Sewage sludge: land utilization and the environment (pp. 237–247). Madison, WI: ASA, CSSA, and SSSA.Google Scholar
  32. Pichtel, J., & Anderson, M. (1997). Trace metal bioavailability in municipal solid waste and sewage sludge composts. Bioresource Technology, 60, 223–229.CrossRefGoogle Scholar
  33. Planquart, P., Bonin, G., Prone, A., & Massiani, C. (1999). Distribution, movement and plant availability of trace metals in soils amended with sewage sludge composts, application to low metal loadings. Science of the Total Environment, 241, 161–179.CrossRefGoogle Scholar
  34. Rhoades, J. K. (1982). Cation exchange capacity. In A. L. Page, et al. (Eds.), Methods of soil analysis. Part 2. Chemical and microbiological properties (2nd ed., pp. 149–158). Madison, WI: Agronomy Monograph 9.Google Scholar
  35. US EPA. (1999). Biosolids generation, use, and disposal in the United States. Washington, DC: Municipal and Industrial Solid Waste Division, Office of Solid Waste, EPA 530-R-99-009.Google Scholar
  36. Vlamis, J., Willams, D. E., Corey, J. E., Page, A. L., & Ganje, T. J. (1985). Zinc and cadmium uptake by barley in field plots fertilized seven years with urban and suburban sludge. Soil Science, 139, 81–87.CrossRefGoogle Scholar
  37. Vig, K., Megharaj, M., Sethunathan, N., & Naidu, R. (2003). Bioavailability and toxicity of cadmium to microorganisms and their activities in soil: a review. Advanced Environmental Research, 8, 121–135.CrossRefGoogle Scholar
  38. Wong, J. W. C., Lai, K. M., Su, D. S., & Fang, M. (2001). Availability of heavy metals for Brassica chinensis growth in an acidic loamy soil amended with a domestic and an industrial sewage sludge. Water, Air and Soil Pollution, 128, 339–353.CrossRefGoogle Scholar
  39. Wong, J. W. C., Li, G. X., & Wong, M. H. (1996). The growth of Brassica chinensis in heavy metal contaminated sewage sludge compost from Hong Kong. Bioresource Technology, 58, 309–313.CrossRefGoogle Scholar
  40. Wu, F., Zhang, G., Dominy, P., Wu, H., & Bachir, D. M. L. (2007). Differences in yield components and kernel Cd accumulation in response to Cd toxicity in four barley genotypes. Chemosphere, 70, 83–92.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Hsuen-Li Chen
    • 1
    • 3
  • Hung-Yu Lai
    • 2
  • Su-Mei Wang
    • 1
    • 3
  • Yu-Chen Kuo
    • 1
    • 3
  • Chih-Jen Lu
    • 1
  1. 1.Department of Environmental EngineeringNational Chung Hsing UniversityTaichungTaiwan
  2. 2.Department of Post-Modern AgricultureMingDao UniversityChanghuaTaiwan
  3. 3.Environmental Protection Bureau of Changhua CountyChanghuaTaiwan

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