Stress responses can be measured at various levels of biological organization, from sub-organism through to ecosystem level. This study aimed to investigate stress protein induction as a sub-organism level stress response in two South African freshwater aquatic macroinvertebrates exposed to detergent linear alkylbenzene sulfonate (LAS). Shrimp Caridina nilotica and limpet Burnupia stenochorias were exposed to a range of LAS concentrations (0, 1, 1.8, 3.2, 6.5 and 12.7 mg/L and 0, 0.6, 1.2, 1.6 and 3.2 mg/L respectively) for 96 h. Surviving organisms were prepared for sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot analysis. In C. nilotica there appeared to be induction of a putative ≈ 70 kDa protein at 12.7 mg/L LAS, and induction of putative ≈45 and ≈40 kDa proteins at concentrations of 6.5 mg/L LAS and above. However, only an Hsp70 protein was detected with anti-Hsp72/Hsc73 at 12.7 mg/L LAS. No protein induction was observed in exposed B. stenochorias, however an Hsp40 protein was detected with anti-Hsp40 in exposed and unexposed limpets.
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The authors wish to thank Ms Caryn McNamara for her advice and assistance. Funding for this research was provided by the Safety and Environmental Assurance Centre, Unilever, Colworth, UK and the Water Research Commission, South Africa.
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Gordon, A.K., Blatch, G.L., Daniel, S. et al. Stress Protein Responses in South African Freshwater Invertebrates Exposed to Detergent Surfactant Linear Alkylbenzene Sulfonate (LAS). Water Air Soil Pollut 193, 123 (2008). https://doi.org/10.1007/s11270-008-9676-1
- Heat shock proteins
- Laundry detergent
- Water quality management