Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effects of Elevated Ozone Concentrations on Thai Jasmine Rice Cultivars (Oryza Sativa L.)

  • 316 Accesses

  • 41 Citations


This study investigated the effects of O3 on growth, yields and physiological characteristics of Thai Jasmine rice cultivars. Rice was exposed to O3 for 7 h day−1 in a closed chamber for 113 days, beginning from seedling until harvest. O3 concentration in each chambers was controlled at 0 ppb, 50 ppb, 100 ppb, 150 ppb and at the ambient level. Effects of O3 on leaf area index (LAI) became obvious at maturity when LAI significantly decreased in the treatments under elevated O3 concentrations. Results in shoot biomass indicated that shoot length was more affected by O3 than shoot dry weight. Root length rather than dry weight was significantly reduced in all cultivars. The most severe damage of O3 was found in photosynthetic components, namely chlorophyll and carotenoid contents, and rate of net photosynthesis. Yield components were also strongly affected by O3. The highest reduction in filled seed per ear was found in the Pathumthani 1 cultivar by 78% when it was exposed to 150 ppb O3 compared to the control (0 ppb). Similarly, 100-grain weight was also reduced as much as 12.3% in this cultivar.

This is a preview of subscription content, log in to check access.


  1. American Public Health Association: 1972, Tentative Method for the Manual Analysis of Oxidizing Substances in the Atmosphere, 44101-02-7OT, Washington, DC.

  2. Bell, J. N. B. and Cox, W. W.: 1975, ‘Atmospheric ozone and plant damage in the United Kingdom’, Environ. Pollut. 8, 163–170.

  3. Biodiversity and Community Rights Action Thailand (Biothai): 2004,

  4. Cooley, D. R. and Manning, W. J.: 1987, ‘The impact of ozone on assimilate pertitioning in plants: A review’, Environ. Pollut. 47, 95–113.

  5. David, T. T., David, M. O., Andrew, A. H. and Lee, E. H.: 1994, ‘ Effects of ozone on crops’, in J. M. David (ed), Tropospheric Ozone, Lewis Publisher, USA, pp 175–206.

  6. Dizengremel, P.: 2001, ‘Effects of ozone on the carbon metabolism of forest tree’, Plant Physiol. Biochem. 39, 729–742.

  7. Finnan, J. M., Jones, M. B. and Burke, J. I.: 1996, ‘A time-concentration study on the effects of ozone on spring wheat (Triticum Aestivum L.) 1’, Effects on Yield. Agric. Ecosystem. Environ. 57, 159–167.

  8. Gelang, J.: 2001, ‘Impact of O3 and CO2 on grain growth and yield of wheat’, Proceedings of the 6th International Conference on Safety in the Port Environment, 8–10 October 2001.

  9. Gelang, J., Sellden, G., Younis, S. and Pleijel, H.: 2001, ‘Effects of ozone on biomass, non-structural carbohydrates and nitrogen in spring wheat with artificially manipulated source/sink ratio’, Environ. Exp. Bot. 46, 155–169.

  10. Gerosa, G., Marzuoli, R., Bussotti, F., Marica, P. and Ballarin-Danti, A.: 2003, ‘Ozone sensitivity of Fagus sylvatica and Fraxinus excelsior young trees in relation to leaf structure and foliar ozone uptake’, Environ. Pollut. 125, 91–98.

  11. Gimeno, B. S., Bermejo, V., Reinert, R. A., Zheng, Y. and Barnes, J. D.: 1999, ‘Adverse effects of ambient ozone on watermelon yield and physiology at rural site in eastern Spain’, New Phytol. 144, 245–260.

  12. Hossain, M. and Pingali, P. L.: 1998, ‘Impact of rice research’, International Rice Research Institute, Philippines, 428 pp.

  13. Kobayashi, K., Okada, M. and Nouchi, I.: 1995, ‘Effects of ozone on dry matter partitioning and yield of Japanses cultivars of rice (Oryza saltiva L.)’, Agric. Ecosystem. Environ. 53, 109–122.

  14. Kobayashi, K. and Okada, M.: 1995, ‘Effects of ozone on the light use of rice (Oryza saltiva L.) plants’, Agric. Ecosystem. Environ. 53, 1–12.

  15. Kuhbauch, W. and Thome, V.: 1989, ‘Nonstructural carbohydrates of wheat stems as influenced by sink-source manipulations’, J. Plant Physiol. 134, 243–250.

  16. Maggs, R. and Ashmore, M. R.: 1998, ‘Growth and yield responses of Pakistan rice (Oryza saltiva L.) cultivars to O3 and NO2’, Environ. Pollut. 103, 159–170.

  17. Manning, W. J. and Feder, W. A.: 1976, ‘Effects of air pollutants on plants’, in S. L. Allen (ed), Surface Level Ozone Exposures and Their Effects on Vegetation, Lewis Publishers, USA, pp. 93–156.

  18. McLaughlin, S. B. and McConathy, R. K.: 1983, ‘Effects of SO2 and O3 on allocation of 4C-labeled photosynthate in Phaseolus vulgaris’, Plant Physiol. 73, 630–635.

  19. Meyer, U., Kollner, B., Willenbrink, J. and Krause, G. H. M.: 2000, ‘Effects of different ozone exposure regimes on photosynthesis, assimilates and thousand grain weight in spring wheat’, Agric. Ecosystem. Environ. 78, 49–55.

  20. Office of Agricultural Economics (OAE): 2004,

  21. Olszyk, D. M. and Wise, C.: 1997, ‘Interactive effects of elevated CO2 and O3 on rice and flacca tomato’, Agric. Ecosystem. Environ. 66, pp. 1–10.

  22. Ostromsky, T., Dimov, I., Tzavetanov, I. and Zlatev, Z.: 2001, ‘Estimation of the wheat losses caused by the tropospheric ozone in Bulgaria and Denmark’, in L. Vulkov, J. Wasniewski and P. Yalamov (eds), Numerical Analysis and Its Applications, Springer-Verlag, Berlin, Heidelberg, pp. 636–643.

  23. Pell, E. J., Eckhard, N. and Eniyedi, A. J.: 1992, ‘Timing of ozone stress and resulting status of ribulose biphosphate carboxylase/oxygenase and associated net photosynthesis’, New Phytol. 120, 397–408.

  24. Pellinen, R., Palva, T. and Kangasjarvi, J.: 1999, ‘Subcellular localization of ozone-induced hydrogen peroxide production in birch (Betula pendula) leaf cell’, Plant J. 20, 349–356.

  25. Pollution Control Department (PCD): 2004,

  26. Rice Research Institute, Thailand: 1999, ‘Report suggestion in variety of Thai rice 75 cultivars’, Department of Agriculture, Thailand, 29 pp.

  27. Sagar, V. K. and William, J. M.: 1988, ‘Atmospheric ozone: Formation and effects on vegetation’, Environ. Pollut. 50, 101–137.

  28. Samuel, M. A., Miles, G. P. and Ellis, B. E.: 2000, ‘Ozone treatment rapidly activates MAP kinase signaling in plants’, Plant J. 22, 367–376.

  29. Sanders, G. E., Dixon, J. and Cobb, A. H.: 1993, ‘Will increasing ozone pollution associated with global climate change alter crop tolerance to herbicides’, Glob. Climat. Change. 56, 83–93.

  30. Toncelli, M. L. and Lorenzini, G.: 1999, ‘Comparative evaluation of biomonitoring techniques of tropospheric ozone’, Environ. Monitor. Assess. 55, 445–458.

  31. Vollenweider, P., Ottiger, M. and Gunthardt-Goerg, M. S.: 2003, ‘Validation of leaf ozone symptoms in natural vegetation using microscopical methods’, Environ. Pollut. 124, 101–118.

  32. Wahid, A., Maggs, R., Shamasi, S. R. M., Bell, J. N. E. and Ashmore, M. R.: 1995a, ‘Effects of air pollution on rice yield in the Pakistan Punjab’, Environ. pollut. 90, 323–329.

  33. Wahid, A., Maggs, R., Shamsi, S. R. A., Bell, J. N. B. and Ashmore, M. R.: 1995b, ‘Air pollution and its impacts on wheat yield in the Pakistan Panjab’, Environ. Pollut. 88, 147–154.

  34. Yoshida, S. C., Forno, D. A., Cock, J. H. and Gomez, J. C.: 1976, Laboratory Manual for Physiological Studies of Rice, The International Rice Research Institute, Philippines, 83 pp.

Download references

Author information

Correspondence to W. Ariyaphanphitak.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ariyaphanphitak, W., Chidthaisong, A., Sarobol, E. et al. Effects of Elevated Ozone Concentrations on Thai Jasmine Rice Cultivars (Oryza Sativa L.). Water Air Soil Pollut 167, 179–200 (2005).

Download citation


  • closed chamber
  • rice (Oryza sativa (L))
  • Thai Jasmine rice
  • tropospheric O3