Water, Air, and Soil Pollution

, Volume 165, Issue 1–4, pp 195–209

Assessment Of Tropical Grasses And Legumes For Phytoremediation Of Petroleum-Contaminated Soils

  • Nicole Merkl
  • Rainer Schultze-Kraft
  • Carmen Infante


Phytoremediation is a promising technology for the clean-up of petroleum-contaminated soils, especially in the tropics where climatic conditions favour plant growth and microbial activity and where financial resources can be limited. The objective of this work was to identify tropical plant species from the eastern savannahs of Venezuela suitable for this technology. Three legumes (Calopogonium mucunoides, Centrosema brasilianum, Stylosanthes capitata) and three grasses (Brachiaria brizantha, Cyperus aggregatus, Eleusine indica) were tested for their ability to stimulate microbial degradation in soil contaminated with 5% (w/w) of a heavy crude oil. In greenhouse experiments, plant biomass production and oil dissipation (total oil and grease, and fraction composition) were analysed after 90 and 180 days incubation. Although previously tested on their tolerance to oil contamination, the legumes died within six to eight weeks. The grasses showed reduced biomass production under the influence of the contaminant. Relative growth rates were higher in contaminated soil indicating a delay in plant growth patterns and development. Soil planted with B. brizantha and C. aggregatus showed a significantly lower oil concentration than non-vegetated soil. Furthermore, a positive correlation between root biomass production and oil degradation was found. Concentration of saturated hydrocarbons was always lower in planted than in unplanted soil. B. brizantha also caused a considerable reduction of aromatics. Based on these results, B. brizantha is recommended for follow-up investigations which could further develop the application of phytoremediation of petroleum-contaminated soils in the tropics.


Brachiaria brizantha bioremediation crude oil degradation oil fractions phytoremediation phytotoxicity 


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  1. Anderson, T. A., Guthrie, E. A. and Walton, B. T.: 1993, ‘Bioremediation in the Rhizosphere’, Environ. Sci. Technol. 27(13), 2630–2636.CrossRefGoogle Scholar
  2. Atlas, R. M. and Bartha, R.: 1998, Microbial Ecology: Fundamentals and Applications, Benjamin Cummings Publ. Co., San Francisco, USA, pp. 393–399.Google Scholar
  3. Bossert, I. and Bartha, R.: 1984, ‘The fate of petroleum in soil ecosystems’, in R. Atlas (ed.) Petroleum Microbiology, Macmillan Pub. Co., New York, USA, pp. 435–474.Google Scholar
  4. Caraballo, L., Urich, J., Vásquez, P., Marcano, P., Muzziotti, R., Rodríguez, A., López, C., Marcano, J., Rodríguez, P., Colombo, P., Quiñones, R. and Asuaje, R.: 1998, ‘Effects of single and/ or combined application of water and oil based drill cuttings in a tropical soil located in Jusepín oil field (Monagas State, Venezuela)’, SPE paper 48920, The 1998 SPE International Conference on Health, Safety, and Environment in Oil & Gas Exploration, Caracas, Venezuela, 7–10 June 1998, Society of Petroleum Engineers, Richardson, Texas, USA.Google Scholar
  5. Chaîneau, C. H., Morel, J. L. and Oudot, J.: 1997, ‘Phytotoxicity and Plant Uptake of Fuel Oil Hydrocarbons’, Environ. Qual. 26, 1478–1483.Google Scholar
  6. Cheng, H. H. and Mulla, D. J.: 1999, ‘The Soil Environment’, in D. C. Adriano, W. T. Jr. Frankenberger, R. C. Sims and J.-M. Bollag (eds.), Bioremediation of Contaminated Soils, American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, Wisconsin, USA, pp. 1–13.Google Scholar
  7. Fitter, A. H. and Hay, R. K. M.: 1987, Environmental Physiology of Plants, Academic Press, London.Google Scholar
  8. Frick, C. M., Farrell, R. E. and Germida, J. J.: 1999, Assessment of Phytoremediation as an In-Situ Technique for Cleaning Oil-Contaminated Sites, Petroleum Technology Alliance of Canada, Calgary.Google Scholar
  9. Gallegos Martínez, M., Gómez Santos, A., González Cruz, L., Montes de Oca García, M. A., Yañez Trujillo, L., Zermeño Eguía Liz, J. A. and Gutiérrez-Rojas, M.: 2000, ‘Diagnostic and resulting approaches to restore petroleum-contaminated soil in a Mexican tropical swamp’, Water Sci. Technol. 42(5-6), 377–384.Google Scholar
  10. Gleba, D., Borisjuk, N. V., Borisjuk, L. G., Kneer, R., Pouley, A., Skarzhinskaya, M., Dushenkov, S., Logendra, S., Gleba, Y. Y. and Raskin, I.: 1999, ‘Use of plant roots for phytoremediation and molecular farming’, National Academy of Science Colloquium 96, 5973–5977.CrossRefGoogle Scholar
  11. Godwin, B. and Thorpe, J.: 2000, Assessment of Phytoremediation as an In Situ-Technique for Cleaning Oil-Contaminated Sites, Final Report, Petroleum Technology Alliance of Canada, Calgary, Canada.Google Scholar
  12. Huesemann, M. H. and Moore, K. O.: 1993, ‘Compositional Changes During Landfarming of Weathered Michigan Crude Oil-Contaminated Soils’, J. Soil Contam. 2(3), 245–262.Google Scholar
  13. Hutchinson, S. L., Schwab, A. P. and Banks, M. K.: 2001, ‘Phytoremediation of aged petroleum sludge: Effect of inorganic fertilizer’, J. Environ. Qual. 30, 395–403.PubMedGoogle Scholar
  14. Itai, C. and Birnbaum, H.: 2002, ‘Synthesis of plant growth regulators by roots’, in Y. Waisel, U. Eshel and U. Kafkafi (eds.), Plant roots – The hidden half, Dekker, New York, USA.Google Scholar
  15. Ivanhoe, L. F.: 1999, ‘Petroleum position of Brazil and Venezuela’, Hubbert Center Newsletter #99/3, M. King Hubbert Center for Petroleum end Supply Studies, Golden, Colorado, USA.Google Scholar
  16. Kirk, J. L., Klironomos, J. N., Lee, H. and Trevors, J. T.: 2002, ‘Phytotoxicity Assay to Assess Plant Species for Phytoremediation of Petroleum-Contaminated Soil’, Bioremediation J. 6, 57–63.CrossRefGoogle Scholar
  17. Lambers, H., Chapin, F. S. and Pons, T. L.: 1998, Plant Physiological Ecology, Springer Verlag, Heidelberg, Germany.Google Scholar
  18. León, N., Infante, C., Arias, M., Márquez, M., Gorrín, A., McMillen, S. J. and Smart, D. R.: 1998, ‘Biodegradability of Venezuelan Crude Oils’, SPE paper 48921, The 1998 SPE International Conference on Health, Safety, and Environment in Oil & Gas Exploration, Caracas, Venezuela, 7–10 June 1998, Society of Petroleum Engineers, Richardson, Texas, USA.Google Scholar
  19. Lin, Q. and Mendelssohn, I. A.: 1998, ‘The combined effects of phytoremediation and biostimulation in enhancing habitat restoration and oil degradation of petroleum contaminated wetlands’, Ecol. Eng. 10(3), 263–274.CrossRefGoogle Scholar
  20. Mager, D. and Hernández-Valencia, I.: 2003, ‘Uso de Brachiaria brizantha y Panicum maximum para fitorremediar suelos contaminados con un crudo de petróleo liviano’, Poster presented at the V. Congreso Venezolano de Ecología, Isla de Margarita, Venezuela.Google Scholar
  21. Merkl, N., Schultze-Kraft, R. and Infante, C.: 2004a, ‘Phytoremediation of petroleum- contaminated soils in the tropics – Preliminary assessment of the potential of species from eastern Venezuela’, J. Appl. Bot. Food Qual. 78(3), 185–192.Google Scholar
  22. Merkl, N., Schultze-Kraft, R. and Infante, C.: 2004b, ‘Phytoremediation in the tropics – The effect of crude oil on the growth of tropical plants’, Bioremediation J. 8(3-4), 177–184.CrossRefGoogle Scholar
  23. Oudot, J., Dupont, J., Haloui, S. and Roquebert, M. F.: 1993, ‘Biodegradation potential of hydrocarbon-assimilating tropical fungi’, Soil Biol. Biochem. 25(9), 1167–1173.CrossRefGoogle Scholar
  24. Paul, E. A. and Clark, F. E.: 1989, Soil Microbiology and Biochemistry, Academic Press, San Diego, USA.Google Scholar
  25. Prado-Jatar, M., Correa, M., Rodríguez-Grau, J. and Carneiro, M.: 1993, ‘Oil sludge farming biodegradation experiment conducted at a tropical site in eastern Venezuela’, Waste Manage. Res. 11, 97–106.CrossRefGoogle Scholar
  26. Rivera-Cruz, M. D., Ferrera-Cerrato, R., Sánchez-Garcia, P., Volke-Haller, V., Fernández-Linares, L. and Rodríguez-Vázquez, R.: 2004, ‘Decontamination of soils polluted with crude petroleum using indigenous microorganisms and alemán grass[Echinochloa polystachya (H.B.K.) Hitchc.]’, Agrociencia 38, 1–12.Google Scholar
  27. Salanitro, J. P., Dorn, P. B., Huesemann, M. H., Moore, K. O., Rhodes, I. A., Jackson, L. M. R., Vipond, T. E., Western, M. M. and Wisniewski, H. L.: 1997, ‘Crude oil hydrocarbon bioremediation and soil ecotoxicity assessment’, Environ. Sci. Technol. 31(6), 1769–1776.CrossRefGoogle Scholar
  28. Schnoor, J. L.: 1997, Phytoremediation, GWRTAC Series – Technology Evaluation Report TE-98-01, Ground-Water Remediation Technologies Analysis Center, Pittsburgh, PA, USA.Google Scholar
  29. Schwendinger, R.: 1968, ‘Reclamation of soil contaminated with oil’, J. Inst. Petrol. 54, 182–192.Google Scholar
  30. Stone, R.: 1940, ‘Bacteria attacking petroleum and oil fractions’, J. Bacteriol. 40, 461.Google Scholar
  31. U.S. EPA: 1994, Test Methods for Evaluating Solid and Hazardous Waste, U.S. Environmental Protection Agency, Washington, DC, USA.Google Scholar
  32. Walton, B. T., Guthrie, E. A. and Hoylman, A. M.: 1994, ‘Toxicant degradation in the rhizosphere’, in T. A. Anderson and J. R. Coats (eds.), Bioremediation through rhizosphere technology, ACS symposium series[563], American Chemical Society, Columbus, Ohio, USA, pp. 11–26.Google Scholar
  33. Wiltse, C. C., Rooney, W. L., Chen, Z., Schwab, A. P. and Banks, M. K.: 1998, ‘Greenhouse evaluation of agronomic and crude oil-phytoremediation potential among alfalfa genotypes’, J. Environ. Qual. 27(1), 169–173.Google Scholar
  34. Yeung, P. Y., Johnson, R. L. and Xu, J. G.: 1997, ‘Biodegradation of Petroleum Hydrocarbons in Soil as Affected by Heating and Forced Aeration’, J. Environ. Qual. 26, 1511–1516.Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Nicole Merkl
    • 1
    • 2
  • Rainer Schultze-Kraft
    • 1
  • Carmen Infante
    • 3
  1. 1.Institute of Plant Production and Agroecology in the Tropics and Subtropics, Department of Biodiversity and Land RehabilitationUniversity of Hohenheim 380CStuttgartGermany
  2. 2.Departamento de Ecología y AmbientePDVSA-Intevep (Centro de Investigación y Apoyo Tecnológico de Petróleos de Venezuela S.A.)CaracasVenezuela
  3. 3.FUNINDES, Unidad de Gestión AmbientalUniversidad Simón Bolivar (USB)CaracasVenezuela

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