Molecular characterization and complete genome of alstroemeria mosaic virus (AlMV)
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Even though alstroemeria mosaic virus (AlMV) is one of the most important viruses affecting alstroemeria plants, its genome is only partially available in public sequence databases. High throughput sequencing (HTS) of RNA from alstroemeria plants with symptoms of mosaic and streaking, collected in Lasso-Ecuador, indicated the presence of AlMV and lily symptomless virus. In this study, we aimed to assemble and characterize the complete genome sequence of AlMV. Reads from Illumina sequencing of ribosomal RNA-depleted total RNA were assembled into contigs that were mapped to the sunflower chlorotic mottle virus genome, revealing the 9974 bp complete genome sequence of AlMV. Multiple sequence alignment of the AlMV polyprotein with close homologs allowed the identification of ten mature proteins P1, HC-Pro, P3, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP. Furthermore, several potyvirus motifs were identified in the AlMV polyprotein including those related to potyvirus aphid transmission 334KMTC337, 592PTK594 and 2800DAG2802. Phylogenetic analysis based in the polyprotein showed that AlMV belongs to the potato virus Y clade and its closest relative is sunflower ring blotch virus. This study describes the first complete genome of AlMV and its placement within the genus Potyvirus, providing valuable information for future studies on this economically important virus.
KeywordsAlMV Alstroemeria Genome Polyprotein Potyvirus
The authors thank Dr. Nathan Richard Walker for revising and improving our manuscript. All bioinformatics analyses were performed in the Rumiñahui high performance cluster of Universidad de las Fuerzas Armadas-ESPE. Biological samples were collected and sequenced under framework contract MAE–DNB–CM–2017–0071.
Conceived the study: FJF. Designed and performed the research: FMY, PG, and FJF. Analyzed data: FMY, and FJF. Curated data: FMY. Wrote the manuscript: FMY, PG. Reviewed the manuscript and supervised the research: FJF. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Research involving human and animal rights
This article does not contain any studies with human participants or animals performed by any of the authors.
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