Identification of a lytic Pseudomonas aeruginosa phage depolymerase and its anti-biofilm effect and bactericidal contribution to serum
Pseudomonas aeruginosa (P. aeruginosa) infection has imposed a great threat to patients with cystic fibrosis. With the emergence of multidrug-resistant P. aeruginosa, developing an alternative anti-microbial strategy is indispensable and more urgent than ever. In this study, a lytic P. aeruginosa phage was isolated from the sewage of a hospital, and one protein was predicted as the depolymerase-like protein by genomic sequence analysis, it includes two catalytic regions, the Pectate lyase_3 super family and Glycosyl hydrolase_28 super family. Further analysis demonstrated that recombinant depolymerase-like protein degraded P. aeruginosa exopolysaccharide and enhanced bactericidal activity mediated by serum in vitro. Additionally, this protein disrupted host bacterial biofilms. All of these results showed that the phage-derived depolymerase-like protein has the potential to be developed into an anti-microbial agent that targets P. aeruginosa.
KeywordsPseudomonas aeruginosa Bacteriophage Depolymerase Biofilm Exopolysaccharide
MLY conducted the identification, expression, and evaluation of the depolymerase and drafted the manuscript. LYN screened and identified the Pa.1193 bacterium. WC performed the exopolysaccharide extraction and staining. GS performed the bacterial biofilm experiment. HTT isolated the IME180 phage. XSZ conducted the depolymerase purification. HY, FH, and ZXLL performed genomic analyses and annotation. TYG, YWG, and MZQ revised the manuscript. BCQ and HF conceived and designed the experiments. All authors read and approved the final manuscript.
This study was supported by the National Natural Science Foundation of China (81572045), the Capital Characteristic Clinic Project of Beijing (Z121107001012127), the Beijing Natural Science Foundation (7142118), and the National Key Research and Development Program of China (2017YFF0108605).
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest to declare.
Research involving human participants and/or animals
All procedures involving animals were in accordance with ethical standards.
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