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Virus Genes

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Molecular characterization of a new recombinant brassica yellows virus infecting tobacco in China

  • Qian Wang
  • Fang-Zheng Xu
  • Lu-Lu An
  • Hai-Ying Xiang
  • Wei-Hua Zhang
  • Guan-Shan LiuEmail author
  • Hao-Bao LiuEmail author
Article
  • 20 Downloads

Abstract

Brassica yellows virus (BrYV), prevalently distributed throughout mainland China and South Korea while triggering serious diseases in cruciferous crops, is proposed to be a new species in the genus Polerovirus within the family Luteoviridae. There are three distinct genotypes (BrYV-A, BrYV-B and BrYV-C) reported in cabbage and radish. Here, we describe a new BrYV isolate infecting tobacco plants in the field, which was named BrYV-NtabQJ. The complete genome sequence of BrYV-NtabQJ is 5741 nt in length, and 89% of the sequence shares higher sequence identities (about 90%) with different BrYV isolates. However, it possesses a quite divergent region within ORF5, which is more close to Beet western yellows virus (BWYV), Beet mild yellowing virus (BMYV) and Beet chlorosis virus (BChV). A significant recombination event was then detected among BrYV-NtabQJ, BrYV-B Beijng isolate (BrYV-BBJ) and BWYV Leonurus sibiricus isolate (BWYV-LS). It is proposed that BrYV-NtabQJ might be an interspecific recombinant between BrYV-BBJ and BWYV-LS, and the recombination might result in the successful aphid transmission of BrYV from cruciferous crops to tobacco. And it also poses new challenges for BrYV diagnosis and the vegetable production.

Keywords

Brassica yellows virus Recombination Tobacco Polerovirus Phylogenic analysis 

Notes

Acknowledgements

This work was supported by the Science Foundation for Young Scholars of Tobacco Research Institute of Chinese Academy of Agricultural Sciences (2016B02), Natural Science Foundation of Shandong Province (ZR2017QC003) and the Fundamental Research Funds for China Agricultural Academy of Sciences (1610232016005).

Author contributions

This work was conceived, designed, performed and written by QW. FX and LA contributed to the sample collection and virus detection. HX and WZ provided valuable suggestions to the detection methods. GL and HL participated in its design and coordination. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Research involving human participants or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11262_2019_1636_MOESM1_ESM.doc (514 kb)
Supplementary material 1 (DOC 514 KB)
11262_2019_1636_MOESM2_ESM.doc (72 kb)
Supplementary material 2 (DOC 72 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Tobacco Research InstituteChinese Academy of Agricultural SciencesQingdaoPeople’s Republic of China
  2. 2.Yunnan Academy of Tobacco ScienceKunmingPeople’s Republic of China
  3. 3.Vegetable and Flower Research Institute of Shandong Academy of Agricultural SciencesJi’nanPeople’s Republic of China

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