Nasal virome of dogs with respiratory infection signs include novel taupapillomaviruses
Using viral metagenomics, we characterized the mammalian virome of nasal swabs from 57 dogs with unexplained signs of respiratory infection showing mostly negative results using the IDEXX Canine Respiratory Disease RealPCR™ Panel. We identified canine parainfluenza virus 5, canine respiratory coronavirus, carnivore bocaparvovirus 3, canine circovirus and canine papillomavirus 9. Novel canine taupapillomaviruses (CPV21-23) were also identified in 3 dogs and their complete genome sequenced showing L1 nucleotide identity ranging from 68.4 to 70.3% to their closest taupapillomavirus relative. Taupapillomavirus were the only mammalian viral nucleic acids detected in two affected dogs, while a third dog was coinfected with low levels of canine parainfluenza 5. A role for these taupapillomavirues in canine respiratory disease remains to be determined.
KeywordsPapillomaviridae Papillomavirus Taupapillomavirus Canine Next generation sequencing Respiratory infection
We acknowledge the support from Vitalant Research Institute and IDEXX laboratories.
ED, CML and EA designed the study. EA, TGP and MAS performed the experiments. ED, EA and XD analyzed the data. ED and EA wrote the manuscript. ED and CML edited the manuscript and provided funding. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have conflict of interests.
All applicable international, national and/or institutional guidelines for the care and use of animals were followed.
- 1.Appel MJ, Binn LN (1987) Canine infectious tracheobronchitis short review: kennel cough. Virus infections of carnivors. Elseiver Science Publishing Co, New YorkGoogle Scholar
- 2.Ditchfield J, Macpherson LW, Zbitnew A (1962) Association of canine adenovirus (Toronto A 26/61) with an outbreak of laryngotracheitis (Kennel cough): a preliminary report. Can Vet J 3:238–247Google Scholar
- 4.Crawford PC, Dubovi EJ, Castleman WL, Stephenson I, Gibbs EP, Chen L, Smith C, Hill RC, Ferro P, Pompey J, Bright RA, Medina MJ, Johnson CM, Olsen CW, Cox NJ, Klimov AI, Katz JM, Donis RO (2005) Transmission of equine influenza virus to dogs. Science 310(5747):482–485. https://doi.org/10.1126/science.1117950 CrossRefGoogle Scholar
- 6.Taxonomy V (2016) https://talk.ictvonline.org/taxonomy/. Accessed February 2 2018
- 8.Chambers VC, Evans CA (1959) Canine oral papillomatosis. I. Virus assay and observations on the various stages of the experimental infection. Cancer Res 19:1188–1195Google Scholar
- 12.Sundberg JP, O’Banion MK, Schmidt-Didier E, Reichmann ME (1986) Cloning and characterization of a canine oral papillomavirus. Am J Vet Res 47(5):1142–1144Google Scholar
- 14.Zhou D, Paul S, Alkhilaiwi F, Clark M, Schlegel R, Yuan H (2016) Canine papillomavirus 20 isolate Ada, complete genome. GenBank. https://www.ncbi.nlm.nih.gov/nucleotide/1008264056. Accessed Feb 2018
- 15.Tisza MJ, Yuan H, Richard S, Buck CB (2016) Canine Papillomavirus 19 isolate tvmb1, complete genome. https://www.ncbi.nlm.nih.gov/nucleotide/1064859043. Accessed Feb 2018
- 16.Tisza MJ, Yuan H, Schlegel R, Buck CB (2016) Genomic sequence of Canine Papillomavirus 19. Genome Announc 4 (6). https://doi.org/10.1128/genomeA.01380-16
- 26.Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New YorkGoogle Scholar
- 28.Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454 Google Scholar
- 38.ICTV Report C (2017) Virus taxonomy: 2017 release Papillomaviridae. ICTV. https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/121/papillomaviridae. 2018
- 43.Joffe DJ, Lelewski R, Weese JS, McGill-Worsley J, Shankel C, Mendonca S, Sager T, Smith M, Poljak Z (2016) Factors associated with development of Canine Infectious Respiratory Disease Complex (CIRDC) in dogs in 5 Canadian small animal clinics. Can Vet J 57(1):46–51Google Scholar