In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
- 15 Downloads
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.
KeywordsGriffithsin Antiviral Porcine epidemic diarrhea virus Lectin
This study was supported by grants from the Special Fund for Agro-scientific Research in the Public Interest (No. 201303046) and the National Natural Science Foundation of China (No. 31772701 and No. 31502105).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 3.Xue J (2014) Investigation of the mechanism of griffithsin (GRFT): a potent HIV entry inhibitor. Dissertations & Theses—GradworksGoogle Scholar
- 11.Kouokam JC, Huskens D, Schols D, Johannemann A, Riedell SK, Walter W, Walker JM, Matoba N, O’Keefe BR, Palmer KE (2011) Investigation of griffithsin’s interactions with human cells confirms its outstanding safety and efficacy profile as a microbicide candidate. PLoS ONE 6(8):e22635CrossRefGoogle Scholar
- 22.Yamaguchi M, Danev R, Nishiyama K, Sugawara K, Nagayama K (2008) Zernike phase contrast electron microscopy of ice-embedded influenza A virus. J Struct Biol 162(2):271–276Google Scholar
- 23.Sougrat R, Bartesaghi A, Lifson JD, Bennett AE, Bess JW, Zabransky DJ, Subramaniam S (2007) Electron tomography of the contact between T cells and SIV/HIV-1: implications for viral entry. PLoS Pathog 3(5):e63Google Scholar
- 24.O’Keefe BR, Giomarelli B, Barnard DL, Shenoy SR, Chan PKS, Mcmahon JB, Palmer KE, Barnett BW, Meyerholz DK, Wohlfordlenane CL (2010) Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae. J Virol 84(5):2511–2521CrossRefGoogle Scholar
- 27.Barton C, Kouokam JC, Lasnik AB, Foreman O, Cambon A, Brock G, Montefiori DC, Vojdani F, Mccormick AA, O’Keefe BR (2014) Activity of and effect of subcutaneous treatment with the broad-spectrum antiviral lectin griffithsin in two laboratory rodent models. Antimicrob Agents Chemother 58(1):120–127CrossRefGoogle Scholar