Development of a reverse genetics system for a feline panleukopenia virus
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Feline panleukopenia virus (FPV) infects cats and can be fatal to kittens. There is evidence that canine parvovirus originated from FPV, which makes FPV important in studies of the family Parvoviridae. In the present study, the entire genome of FPV strain HH-1/86 was converted into a full-length infectious clone (pFPV). The FPV strain HH-1/86 has a 5123-nt single stranded DNA genome with a Y-shaped inverted 3′ terminal repeat (ITR) and a U-shaped inverted 5′ ITR. Feline kidney cells (F81) were transfected with the pFPV clone which contained a genetic marker, and a rescued virus was obtained (rFPV). The rFPV was identified by its cytopathic effects, indirect immunofluorescence, growth curve analysis, western blot assay and hemagglutination, and was indistinguishable from the parent virus. The FPV infectious clone will facilitate the study of pathogenicity and viral replication of FPV and the inter-species transmission of parvoviruses.
KeywordsFeline panleukopenia virus Infectious clone Rescue virus Biological properties
This work was supported in part by the National Key Research and Development Program of China (No. 2016YFD0501004).
SY, YZ and WL conceived and designed the experiments. NC and WZ performed the experiments. JX, YY, HW, YG, and XX analyzed the data. NC and WZ wrote the article. GL managed and submitted the paper.
Funding was provided by National Natural Science Foundation of Jilin Province and Public Welfare (Agricultural) Industry Research Special Program.
Compliance with ethical standards
Conflict of interest
The authors do not have any conflicts of interest.
This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals developed by the China Council on Animal Care. All protocols were approved by the Committee on the Ethics of Animal Experiments of the Institute of Military Veterinary at the Academy of Military Medical Sciences. The approved ID or permit numbers were SCXK-2012-017.
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