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Virus Genes

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Genomic characterisation of Cuiaba and Charleville viruses: arboviruses (family Rhabdoviridae, genus Sripuvirus) infecting reptiles and amphibians

  • Nikos Vasilakis
  • Robert B. Tesh
  • Steven G. Widen
  • Divya Mirchandani
  • Peter J. Walker
Article
  • 25 Downloads

Abstract

Viruses in the family Rhabdoviridae are ecologically very diverse, infecting mammals, birds, reptiles, fish, plants and a wide range of other terrestrial and aquatic invertebrates. The genus Sripuvirus currently comprises five viruses that appear to circulate in transmission cycles involving reptiles and sandflies. Here, we report an analysis of the complete coding sequences of Cuiaba virus (CUIV), isolated from an amphibian (Bufo marinus) collected in Brazil, and Charleville virus (CHVV), isolated from sandflies (Phlebotomus sp.) and lizards (Gehyra australis), collected in Australia. CUIV and CHVV cluster phylogenetically with the sripuviruses in maximum likelihood trees generated from complete L protein (RdRp) sequences. They also share with sripuviruses unique features in genome organisation, including an additional gene (U1) between the matrix protein (M) gene and glycoprotein (G) gene, and an alternative long open reading frame near the start of the G ORF that encodes a predicted transmembrane protein. In view of their phylogenetic relationships, similar genome organisations and similar ecological characteristics, we propose the assignment of CUIV and CHVV as novel members of the genus Sripuvirus.

Keywords

Rhabdovirus Sprivivirus Cuiaba virus Charleville virus Genome architecture 

Notes

Acknowledgements

Work at UTMB was supported by Grant R24 AI120942 (National Institute of Allergy and Infectious Diseases) and contract HHSN272004/D04 from the National Institutes of Health.

Author contributions

PJW, NV and RBT conceived and planned experiments. NV, SGW and DM performed experiments. PJW and NV analysed data. PJW, NV and RBT wrote the manuscript. All authors reviewed and approved the manuscript.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nikos Vasilakis
    • 1
    • 2
    • 3
  • Robert B. Tesh
    • 1
    • 2
    • 3
  • Steven G. Widen
    • 4
  • Divya Mirchandani
    • 5
  • Peter J. Walker
    • 6
  1. 1.Department of Pathology, Center for Biodefence and Emerging Infectious DiseasesThe University of Texas Medical BranchGalvestonUSA
  2. 2.Center for Tropical DiseasesThe University of Texas Medical BranchGalvestonUSA
  3. 3.Institute for Human Infection and ImmunityThe University of Texas Medical BranchGalvestonUSA
  4. 4.Department of Biochemistry and Molecular BiologyThe University of Texas Medical BranchGalvestonUSA
  5. 5.Department of Microbiology and ImmunologyThe University of Texas Medical BranchGalvestonUSA
  6. 6.School of Biological SciencesThe University of QueenslandSt LuciaAustralia

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