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Virus Genes

, Volume 55, Issue 1, pp 43–50 | Cite as

Sensitive and rapid detection of Zika virus by loop-mediated isothermal amplification

  • Jiangtao ZhaoEmail author
  • Ruo FengEmail author
Article

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus, which is a pathogen affecting humans in Africa, Asia, and America. It is necessary to detect ZIKV with a rapid and sensitive molecular method to guide timely treatment. In this study, a loop-mediated isothermal amplification (LAMP) assay was described, which is an attractive option as a fast, sensitive, and specific method for ZIKV detection using the NS5 protein coding region and the envelope protein (EP) coding region as target sequences. Two different techniques, a calcein/Mn2+ complex chromogenic method and real-time turbidity monitoring, were employed. The specificity and sensitivity of the LAMP assay were determined. The assay’s detection limit was 0.5 × 10−9 pmol/µl DNA for NS5 protein coding region and 1.12 × 10−11 pmol/µl DNA for E coding region, respectively, which is a 100-fold increase in sensitivity compared with real-time reverse transcription-polymerase chain reaction (RT-PCR) and conventional PCR. All 12 non-ZIKA respiratory pathogens tested were negative for LAMP detection, indicating the high specificity of the primers for ZIKV. In conclusion, a visual detection LAMP assay was developed, which could be a useful tool for primary quarantine purposes and clinical screening, especially in situations where resources are poor and in point-of-care tests.

Keywords

Zika virus LAMP Sensitivity Specificity Rapid detection 

Notes

Acknowledgements

This work received support from National Natural Science Foundation of China Grant (81401995).

Author contributions

JZ and RF performed the research, analyzed the data, and wrote the paper.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Histology and Embryology, School of Basic Medical SciencesZhengzhou UniversityZhengzhouPeople’s Republic of China

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