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Virus Genes

, Volume 54, Issue 5, pp 706–718 | Cite as

Complete genome sequence analysis of the Malacosoma neustria nucleopolyhedrovirus from Turkey

  • Donus Gencer
  • Remziye Nalcacioglu
  • Zihni Demirbag
  • Ismail DemirEmail author
Article

Abstract

The lackey moth, Malacosoma neustria (Linnaeus, 1758), a worldwide pest, causes extensive economic losses particularly on hazelnut, plum, oak, poplar, and willow trees. A baculovirus, Malacosoma neustria nucleopolyhedrovirus (ManeNPV-T2), has been isolated from the larvae collected in Turkey and appears to have a potential as a microbial control agent. In this study, we describe the complete genome sequence of ManeNPV-T2 and compare it to other sequenced baculovirus genomes. The ManeNPV-T2 genome is a circular double-stranded DNA molecule of 130,202 bp, has 38.2% G + C, and is predicted to contain 131 putative open reading frames (ORFs) each with a coding capacity of more then 50 amino acids. There are 27 ORFs with unknown function of which 6 are unique to ManeNPV-T2. Eleven homologous regions (hrs) and two bro genes (bro-a and bro-b) were identified in the genome. There are two homologues of chaB and nicotinamide riboside kinase-1 genes, separated from themselves with a few nucleotides. Additionally, ac145, thought to be per os infectivity factor (pif) gene, is also found as two homologues. All 38 core genes are found in the ManeNPV-T2 genome. The phylogenetic tree of ManeNPV-T2 in relation to 50 other baculoviruses whose genomes have been completely sequenced showed ManeNPV-T2 to be closely related to the group II NPVs. This study expands our knowledge on baculoviruses, describes the characterization ManeNPV, and ultimately contributes to the registration of this virus as a microbial pesticide.

Keywords

Complete genome Malacosoma neustria NPV isolate Alphabaculovirus Hrs 

Notes

Acknowledgements

This research was supported by a Graduate Scholarship (2211-A) provided to Donus Gencer through the Scientific and Research Council of Turkey (TUBITAK).

Author contributions

Resources: RN, ZD, ID. Conceived and designed the experiments: DG, RN, ZD, ID. Performed the experiments: DG. Analyzed the data: DG, RN. Wrote the paper: DG, RN. Review and editing: ZD, ID.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest to declare.

Research involving human and animal participants

The research described in this paper does not use any human or animal subjects.

Supplementary material

11262_2018_1595_MOESM1_ESM.docx (35 kb)
Supplementary material 1 (DOCX 34 KB)

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Authors and Affiliations

  1. 1.Department of Biology, Faculty of SciencesKaradeniz Technical UniversityTrabzonTurkey

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