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Full genome sequence of a Sathuvachari virus strain isolated in the southwestern-most archipelago of Japan


Two virus strains, tentatively designated as ON-6/P/05 and ON-7/E/05, were isolated from blood samples of healthy cattle in the Yaeyama Islands, located in the southwestern-most region of Japan, in 2005. Ultrastructural observations of infected baby hamster (BHK-21) cells revealed that the viruses had features consistent with those of orbivirus. As with other orbiviruses, the viral genome consists of 10 double-stranded RNA segments. The full genome sequence of ON-6/P/05 was determined and shared high nucleotide and amino acid identities (90.07–98.22% nucleotide identity; 96.16–99.72% amino acid identity) with that of Sathuvachari virus (SVIV), a member of the species Sathuvachari virus of the genus Orbivirus, originally isolated from starlings collected in southern India in 1963. The sequence of segment two of ON-7/E/05 was identical to that of ON-6/P/05. The isolation of SVIV from cattle also indicated that the virus has a wider host range than previously thought. The potential pathogenicity of SVIV in domestic animals should be considered in future disease surveillance within its distribution range.

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We appreciate the staff of Yaeyama Livestock Hygiene Service Center and Okinawa Prefectural Institute of Animal Health for blood sample collection and data acquisition.

Author information

TK and HS contributed to determining the viral sequences. MA and KT conducted the field study and the virus isolation. ST examined the electron micrographs. TK, MA, TY, and MY performed the data analysis and wrote the manuscript. All authors contributed to the improvement of the manuscript and approved the final version.

Correspondence to Tohru Yanase or Makoto Yamakawa.

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The authors declare that they have no conflict of interest.

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Informed consent is not required because no human participants were involved in this article.

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This article does not contain any studies with human participants performed by any of the authors. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Edited by Juergen A Richt.

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Kato, T., Aizawa, M., Takayoshi, K. et al. Full genome sequence of a Sathuvachari virus strain isolated in the southwestern-most archipelago of Japan. Virus Genes 54, 729–732 (2018).

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  • Arbovirus
  • Orbivirus
  • Reoviridae
  • Mosquito-borne
  • Bos taurus