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Full genome sequence of a Sathuvachari virus strain isolated in the southwestern-most archipelago of Japan

Abstract

Two virus strains, tentatively designated as ON-6/P/05 and ON-7/E/05, were isolated from blood samples of healthy cattle in the Yaeyama Islands, located in the southwestern-most region of Japan, in 2005. Ultrastructural observations of infected baby hamster (BHK-21) cells revealed that the viruses had features consistent with those of orbivirus. As with other orbiviruses, the viral genome consists of 10 double-stranded RNA segments. The full genome sequence of ON-6/P/05 was determined and shared high nucleotide and amino acid identities (90.07–98.22% nucleotide identity; 96.16–99.72% amino acid identity) with that of Sathuvachari virus (SVIV), a member of the species Sathuvachari virus of the genus Orbivirus, originally isolated from starlings collected in southern India in 1963. The sequence of segment two of ON-7/E/05 was identical to that of ON-6/P/05. The isolation of SVIV from cattle also indicated that the virus has a wider host range than previously thought. The potential pathogenicity of SVIV in domestic animals should be considered in future disease surveillance within its distribution range.

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References

  1. 1.

    Attoui H, Mertens PPC, Becnel J et al (2011) Family Reoviridae. In: King AMQ, Adams MJ, Lefkowitz EJ, Carstens EB (eds) Virus taxonomy: IXth report of the international committee on taxonomy of viruses. Elsevier, London, pp 541–637

  2. 2.

    Belhouchet M, Mohd Jaafar F, Firth AE, Grimes JM, Mertens PP, Attoui H (2011) Detection of a fourth orbivirus non-structural protein. PLoS ONE 6:e25697

  3. 3.

    Attoui H, Mohd F, Jaafar (2015) Zoonotic and emerging orbivirus infections. Rev Sci Tech 34:353–361

  4. 4.

    International Committee on Taxonomy of Viruses, Virus Taxonomy (2017) Release. https://talk.ictvonline.org/taxonomy/. Accessed 18 July 2018

  5. 5.

    Mohd Jaafar F, Belhouchet M, Belaganahalli M, Tesh RB, Mertens PP, Attoui H (2014) Full-genome characterisation of Orungo, Lebombo and Changuinola viruses provides evidence for co-evolution of orbiviruses with their arthropod vectors. PLoS ONE 9:e86392

  6. 6.

    Maclachlan NJ, Mayo CE, Daniels PW, Savini G, Zientara S, Gibbs EP (2015) Bluetongue. Rev Sci Tech 34:329–340

  7. 7.

    Savini G, Afonso A, Mellor P, Aradaib I, Yadin H, Sanaa M, Wilson W, Monaco F, Domingo M (2011) Epizootic heamorragic disease. Res Vet Sci 91:1–17

  8. 8.

    Goto Y, Miura Y, Kono Y (1988) Serologic evidence for the etiologic role of Chuzan virus in an epizootic of congenital abnormalities with hydranencephaly-cerebellar hypoplasia syndrome of calves in Japan. Am J Vet Res 49:2026–2029

  9. 9.

    Ohashi S, Matsumori Y, Yanase T, Yamakawa M, Kato T, Tsuda T (2004) Evidence of an antigenic shift among Palyam serogroup orbiviruses. J Clin Microbiol 42:4610–4614

  10. 10.

    Shirafuji H, Yanase T, Kato T, Yamakawa M (2012) Genetic and phylogenetic characterization of genome segments 2 and 6 of bluetongue virus isolates in Japan from 1985 to 2008. J Gen Virol 93:1465–1473

  11. 11.

    Kato T, Shirafuji H, Tanaka S, Sato M, Yamakawa M, Tsuda T, Yanase T (2016) Bovine arboviruses in Culicoides biting midges and sentinel cattle in Southern Japan from 2003 to 2013. Transbound Emerg Dis 63:e160–e172

  12. 12.

    Shirafuji H, Kato T, Yamakawa M, Tanaka T, Minemori Y, Yanase T (2017) Characterization of genome segments 2, 3 and 6 of epizootic hemorrhagic disease virus strains isolated in Japan in 1985-2013: identification of their serotypes and geographical genetic types. Infect Genet Evol 53:38–46

  13. 13.

    Ejiri H, Kuwata R, Tsuda Y, Sasaki T, Kobayashi M, Sato Y, Sawabe K, Isawa H (2014) First isolation and characterization of a mosquito-borne orbivirus belonging to the species Umatilla virus in East Asia. Arch Virol 159:2675–2685

  14. 14.

    Ejiri H, Lim CK, Isawa H, Kuwata R, Kobayashi D, Yamaguchi Y, Takayama-Ito M, Kinoshita H, Kakiuchi S, Horiya M, Kotaki A, Takasaki T, Maeda K, Hayashi T, Sasaki T, Kobayashi M, Saijo M, Sawabe K (2015) Genetic and biological characterization of Muko virus, a new distinct member of the species Great Island virus (genus Orbivirus, family Reoviridae), isolated from ixodid ticks in Japan. Arch Virol 160:2965–2977

  15. 15.

    Ohashi S, Yoshida K, Yanase T, Kato T, Tsuda T (2004) Simultaneous detection of bovine arboviruses using single-tube multiplex reverse transcription-polymerase chain reaction. J Virol Methods 120:79–85

  16. 16.

    Kato T, Yanase T, Suzuki M, Katagiri Y, Ikemiyagi K, Takayoshi K, Shirafuji H, Ohashi S, Yoshida K, Yamakawa M, Tsuda T (2016) Monitoring for bovine arboviruses in the most southwestern islands in Japan between 1994 and 2014. BMC Vet Res 12:125

  17. 17.

    Maan S, Rao S, Maan NS, Anthony SJ, Attoui H, Samuel AR, Mertens PP (2007) Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses. J Virol Methods 143:132–139

  18. 18.

    Kapoor A, Tesh RB, Duraisamy R, Popov VL, Travassos da Rosa AP, Lipkin WI (2013) A novel mosquito-borne Orbivirus species found in South-east Asia. J Gen Virol 94:1051–1057

  19. 19.

    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis Version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

  20. 20.

    Ratinier M, Shaw AE, Barry G, Gu Q, Di Gialleonardo L, Janowicz A, Varela M, Randall RE, Caporale M, Palmarini M (2016) Bluetongue virus NS4 protein is an interferon antagonist and a determinant of virus virulence. J Virol 90:5427–5439

  21. 21.

    Sadeghi M, Popov V, Guzman H, Phan TG, Vasilakis N, Tesh R, Delwart E (2017) Genomes of viral isolates derived from different mosquitos species. Virus Res 242:49–57

  22. 22.

    Matsumori Y, Aizawa M, Sakai Y, Inoue D, Kodani M, Tsuha O, Beppu A, Hirashima Y, Kono R, Ohtani A, Yanase T, Shirafuji H, Kato T, Tanaka S, Yamakawa M (2018) Congenital abnormalities in calves associated with Peaton virus infection in Japan. J Vet Diagn Invest (in press)

  23. 23.

    Hayama Y, Moriguchi S, Yanase T, Suzuki M, Niwa T, Ikemiyagi K, Nitta Y, Yamamoto T, Kobayashi S, Murai K, Tsutsui T (2016) Epidemiological analysis of bovine ephemeral fever in 2012-2013 in the subtropical islands of Japan. BMC Vet Res 12:47

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Acknowledgements

We appreciate the staff of Yaeyama Livestock Hygiene Service Center and Okinawa Prefectural Institute of Animal Health for blood sample collection and data acquisition.

Author information

TK and HS contributed to determining the viral sequences. MA and KT conducted the field study and the virus isolation. ST examined the electron micrographs. TK, MA, TY, and MY performed the data analysis and wrote the manuscript. All authors contributed to the improvement of the manuscript and approved the final version.

Correspondence to Tohru Yanase or Makoto Yamakawa.

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The authors declare that they have no conflict of interest.

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Informed consent is not required because no human participants were involved in this article.

Research involving human participants and/or animals

This article does not contain any studies with human participants performed by any of the authors. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Edited by Juergen A Richt.

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Kato, T., Aizawa, M., Takayoshi, K. et al. Full genome sequence of a Sathuvachari virus strain isolated in the southwestern-most archipelago of Japan. Virus Genes 54, 729–732 (2018). https://doi.org/10.1007/s11262-018-1592-x

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Keywords

  • Arbovirus
  • Orbivirus
  • Reoviridae
  • Mosquito-borne
  • Bos taurus