Altered microRNA expression signature in Chikungunya-infected mammalian fibroblast cells
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Chikungunya virus (CHIKV) infection can cause severe arthralgia and chronic arthritis in humans. MicroRNAs (miRNA) have demonstrated their potential use as biomarker in variety of human pathologies and infections. This study was conducted to understand the miRNA signature in early CHIKV infection stages. In the current study, we used TaqMan-based quantitative PCR method to identify the miRNA signature of host response upon CHIKV infection in human and mouse fibroblast cells. The GO enrichment analysis suggests that the putative target genes of these differentially expressed miRNAs are to be involved in RIG-I pathway, TGF-beta-signaling pathway, JAK–STAT-signaling pathway, MAPK-signaling pathway, cytokine–cytokine receptor interactions, and Fc gamma R-mediated phagocytosis. The results obtained in the current study and earlier studies indicate the potential use of miR15, miR-16, miR-17, let-7e, miR-125, miR-99, and miR-23a as a biomarker in CHIKV infection. miRNAs such as miR-15a, miR-16, miR-140, miR-146a, miR-155, miR203, miR223, miR-499, and miR-363 which are implicated in rheumatoid arthritis showed differential regulation in CHIKV infection. The data obtained in this study provide valuable information on CHIKV-induced miRNA expression in mammalian fibroblast cells, and suggest that CHIKV may establish infection by regulating miRNA expression profile.
KeywordsArthralgia CHIKV infection Functional enrichment MicroRNA profiling Target prediction
The authors would like to acknowledge financial support provided by the Indian Council of Medical Research (ICMR), Ministry of Health and Family Welfare, Government of India. The funding agency had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Conceived and designed the experiments: DP and MSP. Performed the experiments: AM, PP, and SA. Analyzed the data: DP and MSP. Wrote the paper: DP and MSP.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The research described in the paper does not use any human or animal subjects.
- 21.R. Kanungo, Indian J. Med. Microbiol. 26, 408 (2008)Google Scholar
- 34.M. Kurowska-Stolarska, S. Alivernini, L.E. Ballantine, D.L. Asquith, N.L. Millar, D.S. Gilchrist, J. Reilly, M. Ierna, A.R. Fraser, B. Stolarski, C. McSharry, A.J. Hueber, D. Baxter, J. Hunter, S. Gay, F.Y. Liew, I.B. McInnes, Proc. Natl. Acad. Sci. USA 108, 11193–11198 (2011)CrossRefPubMedGoogle Scholar