Barley stripe mosaic virus (BSMV) as a virus-induced gene silencing vector in maize seedlings
Barley stripe mosaic virus (BSMV) was the first reported and still widely used virus-induced gene silencing (VIGS) vector for monocotyledons including wheat and barley. Despite BSMV’s reported infectivity on maize (Zea mays), the use of the virus as a vector in maize has not been optimized. Here, we assayed infectivity of BSMV in different maize cultivars by vascular puncture inoculation. Through knockdown of the endogenous host phytoene desaturase gene, we demonstrate for the first time that BSMV can be used as a VIGS vector in maize. This adds BSMV to the repertoire of tools available for functional studies in maize.
KeywordsBarley stripe mosaic virus (BSMV) Virus-induced gene silencing (VIGS) Maize Vascular puncture inoculation (VPI) Phytoene desaturase (PDS)
The project was kindly supported by the Center for Applied Plant Sciences (CAPS), Ohio State University (OSU), and the United States Department of Agriculture-Agricultural Research Service (USDA-ARS). We thank Dr. Dawei Li, China and Dr. Feng Qu, OSU for providing the BSMV binary vector plasmids.
Project research was carried out by SJ under the direction of LS, with technical support and analyses by KW. All authors contributed to and approved the manuscript.
This study was funded by the Center for Applied Plant Sciences at Ohio State University (in kind funding, no Grant No.) and by the USDA-ARS Corn Soybean and Wheat Quality Research Unit in-house appropriated project 3607-22000-013-00D.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
Research involving human and animal participants
This article does not contain any studies with human participants or animals performed by any of the authors.
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