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Virus Genes

, Volume 54, Issue 4, pp 616–620 | Cite as

Barley stripe mosaic virus (BSMV) as a virus-induced gene silencing vector in maize seedlings

  • Sridhar Jarugula
  • Kristen Willie
  • Lucy R. Stewart
Article

Abstract

Barley stripe mosaic virus (BSMV) was the first reported and still widely used virus-induced gene silencing (VIGS) vector for monocotyledons including wheat and barley. Despite BSMV’s reported infectivity on maize (Zea mays), the use of the virus as a vector in maize has not been optimized. Here, we assayed infectivity of BSMV in different maize cultivars by vascular puncture inoculation. Through knockdown of the endogenous host phytoene desaturase gene, we demonstrate for the first time that BSMV can be used as a VIGS vector in maize. This adds BSMV to the repertoire of tools available for functional studies in maize.

Keywords

Barley stripe mosaic virus (BSMV) Virus-induced gene silencing (VIGS) Maize Vascular puncture inoculation (VPI) Phytoene desaturase (PDS) 

Notes

Acknowledgements

The project was kindly supported by the Center for Applied Plant Sciences (CAPS), Ohio State University (OSU), and the United States Department of Agriculture-Agricultural Research Service (USDA-ARS). We thank Dr. Dawei Li, China and Dr. Feng Qu, OSU for providing the BSMV binary vector plasmids.

Author contributions

Project research was carried out by SJ under the direction of LS, with technical support and analyses by KW. All authors contributed to and approved the manuscript.

Funding

This study was funded by the Center for Applied Plant Sciences at Ohio State University (in kind funding, no Grant No.) and by the USDA-ARS Corn Soybean and Wheat Quality Research Unit in-house appropriated project 3607-22000-013-00D.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11262_2018_1569_MOESM1_ESM.pptx (6.1 mb)
Supplementary material 1 (PPTX 6265 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center for Applied Plant SciencesOhio State UniversityWoosterUSA
  2. 2.Corn, Soybean, and Wheat Quality Research UnitUSDA-ARSWoosterUSA
  3. 3.Department of Plant PathologyWashington State University, Irrigated Agriculture Research and Extension centerProsserUSA

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