Virus Genes

, Volume 54, Issue 4, pp 616–620 | Cite as

Barley stripe mosaic virus (BSMV) as a virus-induced gene silencing vector in maize seedlings

  • Sridhar Jarugula
  • Kristen Willie
  • Lucy R. StewartEmail author


Barley stripe mosaic virus (BSMV) was the first reported and still widely used virus-induced gene silencing (VIGS) vector for monocotyledons including wheat and barley. Despite BSMV’s reported infectivity on maize (Zea mays), the use of the virus as a vector in maize has not been optimized. Here, we assayed infectivity of BSMV in different maize cultivars by vascular puncture inoculation. Through knockdown of the endogenous host phytoene desaturase gene, we demonstrate for the first time that BSMV can be used as a VIGS vector in maize. This adds BSMV to the repertoire of tools available for functional studies in maize.


Barley stripe mosaic virus (BSMV) Virus-induced gene silencing (VIGS) Maize Vascular puncture inoculation (VPI) Phytoene desaturase (PDS) 



The project was kindly supported by the Center for Applied Plant Sciences (CAPS), Ohio State University (OSU), and the United States Department of Agriculture-Agricultural Research Service (USDA-ARS). We thank Dr. Dawei Li, China and Dr. Feng Qu, OSU for providing the BSMV binary vector plasmids.

Author contributions

Project research was carried out by SJ under the direction of LS, with technical support and analyses by KW. All authors contributed to and approved the manuscript.


This study was funded by the Center for Applied Plant Sciences at Ohio State University (in kind funding, no Grant No.) and by the USDA-ARS Corn Soybean and Wheat Quality Research Unit in-house appropriated project 3607-22000-013-00D.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11262_2018_1569_MOESM1_ESM.pptx (6.1 mb)
Supplementary material 1 (PPTX 6265 KB)


  1. 1.
    P.S. Schnable, D. Ware, R.S. Fulton, J.C. Stein, F.S. Wei, S. Pasternak, C.Z. Liang, J.W. Zhang, L. Fulton, T.A. Graves, P. Minx, A.D. Reily, L. Courtney, S.S. Kruchowski, C. Tomlinson, C. Strong, K. Delehaunty, C. Fronick, B. Courtney, S.M. Rock, E. Belter, F.Y. Du, K. Kim, R.M. Abbott, M. Cotton, A. Levy, P. Marchetto, K. Ochoa, S.M. Jackson, B. Gillam, W.Z. Chen, L. Yan, J. Higginbotham, M. Cardenas, J. Waligorski, E. Applebaum, L. Phelps, J. Falcone, K. Kanchi, T. Thane, A. Scimone, N. Thane, J. Henke, T. Wang, J. Ruppert, N. Shah, K. Rotter, J. Hodges, E. Ingenthron, M. Cordes, S. Kohlberg, J. Sgro, B. Delgado, K. Mead, A. Chinwalla, S. Leonard, K. Crouse, K. Collura, D. Kudrna, J. Currie, R.F. He, A. Angelova, S. Rajasekar, T. Mueller, R. Lomeli, G. Scara, A. Ko, K. Delaney, M. Wissotski, G. Lopez, D. Campos, M. Braidotti, E. Ashley, W. Golser, H. Kim, S. Lee, J.K. Lin, Z. Dujmic, W. Kim, J. Talag, A. Zuccolo, C. Fan, A. Sebastian, M. Kramer, L. Spiegel, L. Nascimento, T. Zutavern, B. Miller, C. Ambroise, S. Muller, W. Spooner, A. Narechania, L.Y. Ren, S. Wei, S. Kumari, B. Faga, M.J. Levy, L. McMahan, P. Van Buren, M.W. Vaughn, K. Ying, C.T. Yeh, S.J. Emrich, Y. Jia, A. Kalyanaraman, A.P. Hsia, W.B. Barbazuk, R.S. Baucom, T.P. Brutnell, N.C. Carpita, C. Chaparro, J.M. Chia, J.M. Deragon, J.C. Estill, Y. Fu, J.A. Jeddeloh, Y.J. Han, H. Lee, P.H. Li, D.R. Lisch, S.Z. Liu, Z.J. Liu, D.H. Nagel, M.C. McCann, P. SanMiguel, A.M. Myers, D. Nettleton, J. Nguyen, B.W. Penning, L. Ponnala, K.L. Schneider, D.C. Schwartz, A. Sharma, C. Soderlund, N.M. Springer, Q. Sun, H. Wang, M. Waterman, R. Westerman, T.K. Wolfgruber, L.X. Yang, Y. Yu, L.F. Zhang, S.G. Zhou, Q. Zhu, J.L. Bennetzen, R.K. Dawe, J.M. Jiang, N. Jiang, G.G. Presting, S.R. Wessler, S. Aluru, R.A. Martienssen, S.W. Clifton, W.R. McCombie, R.A. Wing, R.K. Wilson, Science 326, 1112 (2009)CrossRefPubMedGoogle Scholar
  2. 2.
    C.N. Hirsch, J.M. Foerster, J.M. Johnson, R.S. Sekhon, G. Muttoni, B. Vaillancourt, F. Penagaricano, E. Lindquist, M.A. Pedraza, K. Barry, N. de Leon, S.M. Kaeppler, C.R. Buell, Plant Cell 26, 121 (2014)CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    J.A. Martin, N.V. Johnson, S.M. Gross, J. Schnable, X.D. Meng, M. Wang, D. Coleman-Derr, E. Lindquist, C.L. Wei, S. Kaeppler, F. Chen, Z. Wang, Sci. Rep. 4, 59 (2014)Google Scholar
  4. 4.
    B. Wang, E. Tseng, M. Regulski, T.A. Clark, T. Hon, Y.P. Jiao, Z.Y. Lu, A. Olson, J.C. Stein, D. Ware, Nat. Commun. 7, 11708 (2016)CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    S. Jarugula, S.R. Charlesworth, F. Qu, L.R. Stewart, Arch. Virol. 161, 2291 (2016)CrossRefPubMedGoogle Scholar
  6. 6.
    H. Ramanna, X.S. Ding, R.S. Nelson, Methods Mol. Biol. 975, 15 (2013)CrossRefPubMedGoogle Scholar
  7. 7.
    S.R. Scofield, R.S. Nelson, Plant Physiol. 149, 152 (2009)CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    L.M. Benavente, X.S. Ding, M.G. Redinbaugh, R.S. Nelson, P. Balint-Kurti, Maydica 57, 206 (2012)Google Scholar
  9. 9.
    L.M. Benavente, S.R. Scofield, New Phytol. 189, 364 (2011)CrossRefGoogle Scholar
  10. 10.
    X.S. Ding, W.L. Schneider, S.R. Chaluvadi, M.A.R. Mian, R.S. Nelson, Mol. Plant Microbe Interact. 19, 1229 (2006)CrossRefPubMedGoogle Scholar
  11. 11.
    R.S. Nelson, X. Ding, K. Ballard, S.R. Chaluvadi, Phytopathology 97, S83 (2007)Google Scholar
  12. 12.
    Y. Shi, Y.H. Qin, Y.Y. Cao, H. Sun, T. Zhou, Y.G. Hong, Z.F. Fan, Eur. J. Plant Pathol. 131, 317 (2011)CrossRefGoogle Scholar
  13. 13.
    K. van der Linde, C. Kastner, J. Kumlehn, R. Kahmann, G. Doehlemann, New Phytol. 189, 471 (2011)CrossRefPubMedGoogle Scholar
  14. 14.
    Y. Mei, C.Q. Zhang, B.M. Kernodle, J.H. Hill, S.A. Whitham, Plant Physiol. 171, 760 (2016)PubMedPubMedCentralGoogle Scholar
  15. 15.
    X.X. R.Wang, N. Yang, X.D. Wang, R.S. Liu, W.M. Nelson, Z.F. Li, T. Fan, Zhou, Plant J. 86, 102 (2016)CrossRefGoogle Scholar
  16. 16.
    M. Bruun-Rasmussen, C.T. Madsen, S. Jessing, M. Albrechtsen, Mol. Plant Microbe Interact. 20, 1323 (2007)CrossRefPubMedGoogle Scholar
  17. 17.
    R. Delventhal, N. Zellerhoff, U. Schaffrath, Plant Signal. Behav. 6, 867 (2011)CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    I. Hein, M. Barciszewska-Pacak, K. Hrubikova, S. Williamson, M. Dinesen, I.E. Soenderby, S. Sundar, A. Jarmolowski, K. Shirasu, C. Lacomme, Plant Physiol. 138, 2155 (2005)CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    S. Holzberg, P. Brosio, C. Gross, G.P. Pogue, Plant J. 30, 315 (2002)CrossRefPubMedGoogle Scholar
  20. 20.
    A. Pacak, K. Geisler, B. Jorgensen, M. Barciszewska-Pacak, L. Nilsson, T.H. Nielsen, E. Johansen, M. Gronlund, I. Jakobsen, M. Albrechtsen, Plant Methods 6, 26 (2010)CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    S.R. Scofield, L. Huang, A.S. Brandt, B.S. Gill, Plant Physiol 138, 2165 (2005)CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    H.A. Tufan, F.L. Stefanato, G.R. McGrann, R. MacCormack, L.A. Boyd, J. Plant Physiol. 168, 990 (2011)CrossRefPubMedGoogle Scholar
  23. 23.
    C. Yuan, C. Li, L. Yan, A.O. Jackson, Z. Liu, C. Han, J. Yu, D. Li, PLoS ONE 6, e26468 (2011)CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    D.R. Pring, Phytopathology 64, 64 (1974)CrossRefGoogle Scholar
  25. 25.
    R. Louie, Phytopathology 85, 139 (1995)CrossRefGoogle Scholar
  26. 26.
    M.G. Redinbaugh, R. Louie, P. Ngwira, R. Edema, D.T. Gordon, D.M. Bisaro, J. Virol. Methods 98, 135 (2001)CrossRefPubMedGoogle Scholar
  27. 27.
    M. Senthil-Kumar, K.S. Mysore, Nat. Protoc. 9, 1549 (2014)CrossRefPubMedGoogle Scholar
  28. 28.
    P. Saxena, Y.C. Hsieh, V.Y. Alvarado, F. Sainsbury, K. Saunders, G.P. Lomonossoff, H.B. Scholthof, Plant Biotech. J. 9, 703 (2011)CrossRefGoogle Scholar
  29. 29.
    L.R. Stewart, S. Jarugula, Y. Zhao, F. Qu, D. Marty, Virology 504, 88 (2017)CrossRefPubMedGoogle Scholar
  30. 30.
    R. Louie, M.W. Jones, R.J. Anderson, M.G. Redinbaugh, Crop Sci. 42, 991 (2002)CrossRefGoogle Scholar
  31. 31.
    J.L. Zambrano, M.W. Jones, E. Brenner, D.M. Francis, A. Tomas, M.G. Redinbaugh, Theor. Appl. Genet. 127, 867 (2014)CrossRefPubMedGoogle Scholar
  32. 32.
    M. Jones, E. Boyd, M. Redinbaugh, Theor. Appl. Genet. 123, 729 (2011)CrossRefPubMedGoogle Scholar
  33. 33.
    L.R. Stewart, M.A. Haque, M.W. Jones, M.G. Redinbaugh, Mol. Breeding 31, 289 (2013)CrossRefGoogle Scholar
  34. 34.
    D.G. Gibson, L. Young, R.Y. Chuang, J.C. Venter, C.A. Hutchison, H.O. Smith, Nat. Methods 6, 343 (2009)CrossRefPubMedGoogle Scholar
  35. 35.
    M.K. Brakke, Annu. Rev. Phytopathol. 22, 77 (1984)CrossRefGoogle Scholar
  36. 36.
    H.H. McKinney, L.W. Greeley, Biological Characteristics of Barley Stripe-Mosaic Virus Strains and Their Evolution (United States Department of Agriculture, Washington, 1965)Google Scholar
  37. 37.
    G. Sprague, H. McKinney, L. Greeley, Science 141, 1052 (1963)CrossRefPubMedGoogle Scholar
  38. 38.
    A. Manoli, A. Sturaro, S. Trevisan, S. Quaggiotti, A. Nonis, J. Plant Physiol. 169, 807 (2012)CrossRefPubMedGoogle Scholar
  39. 39.
    M.W. Pfaffl, G.W. Horgan, L. Dempfle, Nucleic Acids Res. 30, e36 (2002)CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    J.M. Tuomi, F. Voorbraak, D.L. Jones, J.M. Ruijter, Methods 50, 313 (2010)CrossRefPubMedGoogle Scholar
  41. 41.
    A. Pacak, P.M. Strozycki, M. Barciszewska-Pacak, M. Alejska, C. Lacomme, A. Jarmołowski, Z. Szweykowska-Kulińska, M. Figlerowicz, Arch. Virol. 155, 169 (2010)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center for Applied Plant SciencesOhio State UniversityWoosterUSA
  2. 2.Corn, Soybean, and Wheat Quality Research UnitUSDA-ARSWoosterUSA
  3. 3.Department of Plant PathologyWashington State University, Irrigated Agriculture Research and Extension centerProsserUSA

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