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Virus Genes

, Volume 54, Issue 4, pp 591–595 | Cite as

First detection of influenza A virus genes from wild raccoons in Japan

  • Emi Yamaguchi
  • Kei Fujii
  • Haruko Ogawa
  • Kunitoshi Imai
Article
  • 126 Downloads

Abstract

Serological surveys have shown that wild raccoons are exposed to influenza A viruses (IAVs); however, no genetic evidence for this IAV infection has been found. In the present study, we first detected IAV genes in wild raccoons captured during periods other than the wintering season of migratory waterfowl and epidemic season of influenza in Japan. Viral matrix (M) and nucleoprotein (NP) genes were detected by a conventional reverse transcription-polymerase chain reaction assay from three suckling siblings and one juvenile without any noticeable clinical signs, although the NP gene could not be detected from one sibling. The sequences of M gene fragments detected from the rectal swabs of three suckling siblings were comparable with each other but different from those detected from the nasal swab of the juvenile raccoon caught from a different site. The sequences of NP gene fragments detected from two suckling siblings were also comparable. These genetic evidences suggest that IAV is maintained among raccoon populations in the northern part of Japan. Further genetic and virological investigation of IAV infection in wild raccoons is needed to better understand the IAV ecology in the field.

Keywords

Influenza A virus Raccoon (Procyon lotorWild mammals Gene detection Japan 

Abbreviations

HA

Hemagglutinin

IAV

Influenza A virus

NA

Neuraminidase

HPAI

Highly pathogenic avian influenza;

RT-PCR

Reverse transcription-polymerase chain reaction

M gene

Matrix gene

NP gene

Nucleoprotein gene

NS

Nonstructural protein

PB1

Polymerase basic protein 1

PB2

Polymerase basic protein 2

PA

Polymerase acid protein

Notes

Acknowledgements

We would like to thank municipalities for collecting raccoon samples and the Emerging and Re-emerging Infectious Diseases Unit members in Obihiro university of Agriculture and Veterinary Medicine for their assistance. We would also like to thank Ms. Sachiko Matsuda and Dr. Dao Duy Tung of our unit for their excellent technical assistance. This work was partially supported by KAKENHI (Grant Number 15K07737) of the Japan Society for the Promotion of Science (JSPS).

Author Contributions

EY and KI conceived and designed the study; EY performed the experiments and sequencing analysis; EY and KI contributed to sample collection. All authors contributed to the writing and revision of the manuscript and approved the final one.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal participants

This article does not contain any studies with human participants performed by any of the authors. Animals utilized for this research were collected in accordance with animal experiments committee of the Obihiro University of Agriculture and Veterinary Medicine.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Research Unit for Emerging and Re-emerging Infectious DiseasesObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.Animal Research CenterHokkaido Research OrganizationShintokuJapan

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