Newcastle disease virus-like particles induce dendritic cell maturation and enhance viral-specific immune response
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Circulating of genotype VII Newcastle disease virus (NDV) is a great threat to the poultry industry worldwide. Virus-like particles (VLPs) are increasingly being considered as potential viral vaccines due to their safety and efficacy. In this study, we analyzed in vitro the stimulatory effects of VLPs containing the matrix and hemagglutinin-neuraminidase of genotype VII NDV on dendritic cells (DCs) and evaluated their immunogenicity in mice. The results showed that immature bone marrow-derived dendritic cells (BMDCs) responded to stimulation with VLPs by up-regulating expressions of MHC II, CD40, CD80, and CD86 molecules and by increasing the cytokine secretions of TNF-α, IFN-γ, IL-6, and IL-12p70. Besides, VLPs enhanced the immunostimulatory capacity of DCs to stimulate autologous T cell proliferation. Furthermore, VLPs can induce efficient humoral and cellular immune responses, and recruit mature DCs to the spleen in C57BL/6 mice, as shown by an obvious increase in double-positive proliferation of splenic CD11c+CD86+ cells. These data indicate that NDV VLPs can be a valuable candidate for NDV vaccine development.
KeywordsNewcastle disease virus Virus-like particle Dendritic cell Immune response
This work was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303033) and the National Natural Science Foundation of China (31272561, 31472195, 31402195).
JQ, JD, YC, RY, YS, CX, XX, and ZD conceived and designed the experiments; JQ, JD, YS, and XX performed the experiments; JQ, YC, JW, RY analyzed the data; YS, CX, JW, CD, SY, XL, SH, and ZD contributed reagents/materials/analysis tools; JQ, XX, and YC wrote the paper. RY, XL, YC, and ZD requested financial support. All authors read and approved the manuscript.
Compliance with ethical standards
Conflict of interest
The authors have declared that no competing interests exist.
This study was conducted in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Ministry of Science and Technology of the People’s Republic of China. The protocols for animal studies were approved by the Committee on the Ethics of Animal Experiments of Jilin University (approval numbers 2015047815-1 for mice).
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