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Virus Genes

, Volume 53, Issue 3, pp 446–453 | Cite as

Rice grassy stunt virus p5 interacts with two protein components of the plant-specific CBL–CIPK Ca+2 signaling network of rice

  • Guihong Xiong
  • Xiaojuan Liu
  • Ping Qiu
  • Xiaoyong Wu
  • Zhenguo Du
  • Jie Zhang
  • Liang Yang
  • Zujian Wu
Article

Abstract

Rice grassy stunt virus (RGSV) is a tenuivirus posing a threat to rice production in many South, Southeast, and East Asian countries. To date, no host factor interacting with RGSV has been reported. In this study, we screened a rice cDNA library with the GAL4-based yeast two-hybrid system using RGSV p5 as the bait. One of the candidate host factors interacting with RGSV p5 was found to be CBL-interacting protein kinase 25 (OsCIPK25), a member of the plant-specific CBL–CIPK Ca2+ signaling network. The interaction between RGSV p5 and OsCIPK25, as well as OsCIPK5, which is closely related to OsCIPK25, was confirmed by their cellular co-localization and by a bimolecular fluorescence complementation assay in Nicotiana benthamiana cells. Given the importance of CIPKs in the regulation of ion homeostasis and the resemblance of RGSV symptoms to potassium deficiency in rice, we evaluated potassium content of RGSV-infected rice and found it to be much lower than that in the healthy rice.

Keywords

Rice grassy stunt virus Interaction CBL–CIPK Potassium metabolism 

Notes

Acknowledgements

This work was supported by Grants from the National Basic Research Program 973 (2014CB138402), Natural Science Foundation of Fujian, China (2016J05071), Natural Science Foundation of China (31301640), and FAFU Science Fund for Distinguished Young Scholars (xjq201622).

Author contributions

G.H.X. and X.J.L. conducted the yeast two-hybrid experiment; G.H.X. and P.Q. conducted the BiFC and cellular localization experiments; X.Y.W. conducted the K+ quantification experiments; J.Z. contributed in vector construction; Z.G.D. and Z.J.W. designed the experiments and wrote the paper.

Compliance with ethical standards

Conflict of interest

The author declares no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Guihong Xiong
    • 1
  • Xiaojuan Liu
    • 1
  • Ping Qiu
    • 1
  • Xiaoyong Wu
    • 1
  • Zhenguo Du
    • 1
  • Jie Zhang
    • 1
  • Liang Yang
    • 1
  • Zujian Wu
    • 1
  1. 1.State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Province Key Laboratory of Plant Virology, Institute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouChina

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