Advertisement

Virus Genes

, Volume 53, Issue 3, pp 352–356 | Cite as

A novel Enterovirus 96 circulating in China causes hand, foot, and mouth disease

  • Yi Xu
  • Yisuo Sun
  • Jinmin Ma
  • Shuru Zhou
  • Wei Fang
  • Jiawei Ye
  • Limei Tan
  • Jingkai Ji
  • Dan Luo
  • Liqiang Li
  • Jiandong Li
  • Chunxiao Fang
  • Na Pei
  • Shuo Shi
  • Xin Liu
  • Hui Jiang
  • Sitang GongEmail author
  • Xun XuEmail author
Article

Abstract

Enterovirus 96 (EV-96) is a recently described member of the species Enterovirus C and is associated with paralysis and myelitis. In this study, using metagenomic sequencing, we identified a new enterovirus 96 strain (EV-96-SZ/GD/CHN/2014) as the sole pathogen causing hand, foot, and mouth disease (HFMD). A genomic comparison showed that EV-96-SZ/GD/CHN/2014 is most similar to the EV-96-05517 strain (85% identity), which has also been detected in Guangdong Province. This is the first time that metagenomic sequencing has been used to identify an EV-96 strain shown to be associated with HFMD.

Keywords

Enterovirus 96 Metagenomics HFMD Genome sequence Evolution 

Notes

Authors’ contributions

STG, YX, and XX designed the experiments; YSS, JMM, and SRZ wrote the manuscript; WF, JJ, LQL, and JDL performed the experiments; JWY, LMT, DL, and FCX contributed to the clinical diagnosis and collected the samples; NP, SS, XL, and HJ coordinated the genomic sequencing and conducted the phylogenetic analyses. All authors have read and approved the final manuscript.

Funding

This study was supported by the Guangzhou Bureau of Science and Telecommunications Fund (2014Y2-00197), the Technological Innovation Plan of Shenzhen—Technology Development Project (No. CXZZ20140904154910774), and a Grant from the Ministry of Science and Technology of the People’s Republic of China (2011DFA33220).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures involving human participants were in accordance with ethical standards.

Supplementary material

11262_2017_1431_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 17 kb)

References

  1. 1.
    T. Hamaguchi, H. Fujisawa, K. Sakai, S. Okino, N. Kurosaki, Y. Nishimura, H. Shimizu, M. Yamada, Emerg. Infect. Dis. 14, 828 (2008)CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    J. Zhang, J. Sun, Z. Chang, W. Zhang, Z. Wang, Z. Feng, Biomed Env. Sci 24, 214 (2011)Google Scholar
  3. 3.
    H.P. Tsai, S.W. Huang, F.L. Wu, P.H. Kuo, S.M. Wang, C.C. Liu, I.J. Su, J.R. Wang, J. Med. Microbiol. 60, 1360 (2011)CrossRefPubMedGoogle Scholar
  4. 4.
    M. Rakoto-Andrianarivelo, S. Guillot, J. Iber, J. Balanant, B. Blondel, F. Riquet, J. Martin, O. Kew, B. Randriamanalina, L. Razafinimpiasa, D. Rousset, F. Delpeyroux, PLoS Pathog. 3, e191 (2007)CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    N.J. Knowles, T. Hovi, T. Hyypiä, A.M.Q. King, A.M. Lindberg, M.A. Pallansch, A.C. Palmenberg, P. Simmonds, T. Skern, G. Stanway et al., Virus taxonomy: Ninth report of the International Committee on Taxonomy of Viruses (Elsevier, San Diego, 2012)Google Scholar
  6. 6.
    M.S. Oberste, K. Maher, A.J. Williams, N. Dybdahl-Sissoko, B.A. Brown, M.S. Gookin, S. Penaranda, N. Mishrik, M. Uddin, M.A. Pallansch, J. Gen. Virol. 87, 119 (2006)CrossRefPubMedGoogle Scholar
  7. 7.
    M. Arita, S.L. Zhu, H. Yoshida, T. Yoneyama, T. Miyamura, H. Shimizu, J. Virol. 79, 12650 (2005)CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    S. Blomqvist, A. Paananen, C. Savolainen-Kopra, T. Hovi, M. Roivainen, J. Clin. Microbiol. 46, 2410 (2008)CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    W.A. Nix, N. Khetsuriani, S. Penaranda, K. Maher, L. Venczel, Z. Cselko, M.C. Freire, D. Cisterna, C.L. Lema, P. Rosales, J.R. Rodriguez, W. Rodriguez, P. Halkyer, O. Ronveaux, M.A. Pallansch, M.S. Oberste, J. Gen. Virol. 94, 2017 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    L.N. Apostol, T. Imagawa, A. Suzuki, Y. Masago, S. Lupisan, R. Olveda, M. Saito, T. Omura, H. Oshitani, Virus Genes 45, 207 (2012)CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    T. Smura, S. Blomqvist, A. Paananen, T. Vuorinen, Z. Sobotova, V. Bubovica, O. Ivanova, T. Hovi, M. Roivainen, J. Gen. Virol. 88, 2520 (2007)CrossRefPubMedGoogle Scholar
  12. 12.
    J. Lu, H. Zheng, Y. Zhang, X. Guo, D. Wu, H. Li, L. Liu, H. Zeng, L. Yi, L. Fang, Y. Mo, W. Xu, C. Ke, PLoS ONE 9, e86877 (2014)CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    A. Xu, Z. Tao, H. Wang, Y. Zhang, L. Song, Y. Li, G. Liu, Y. Liu, H. Yang, Q. Fan, F. Ji, Y. Zhang, J. Yang, L. Feng, W. Xu, Z. Zhao, Virus Genes 42, 323 (2011)CrossRefPubMedGoogle Scholar
  14. 14.
    A. Cui, C. Xu, X. Tan, Y. Zhang, Z. Zhu, N. Mao, Y. Lu, W. Xu, PLoS ONE 8, e61451 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Y. Peng, H.C. Leung, S.M. Yiu, F.Y. Chin, Bioinformatics 28, 1420 (2012)CrossRefPubMedGoogle Scholar
  16. 16.
    W. Liu, J. Zhai, J. Liu, Y. Xie, Virus Genes 40, 222 (2010)CrossRefPubMedGoogle Scholar
  17. 17.
    M. Oberste, D. Schnurr, K. Maher, S. al-Busaidy, and M. Pallansch. J. Gen. Virol. 82, 409 (2001)CrossRefPubMedGoogle Scholar
  18. 18.
    M.S. Oberste, K. Maher, S.M. Michele, G. Belliot, M. Uddin, M.A. Pallansch, J. Gen. Virol. 86, 445 (2005)CrossRefPubMedGoogle Scholar
  19. 19.
    M.S. Oberste, K. Maher, W.A. Nix, S.M. Michele, M. Uddin, D. Schnurr, C. al-Busaidy, S. Akoua-Koffi, M.A. Pallansch, Virus Res. 128, 34 (2007)CrossRefPubMedGoogle Scholar
  20. 20.
    M.S. Oberste, K. Maher, D. Schnurr, M.R. Flemister, J.C. Lovchik, H. Peters, W. Sessions, C. Kirk, N. Chatterjee, S. Fuller, J.M. Hanauer, M.A. Pallansch, J. Gen. Virol. 85, 2577 (2004)CrossRefPubMedGoogle Scholar
  21. 21.
    N. Junttila, N. Leveque, J.P. Kabue, G. Cartet, F. Mushiya, J.J. Muyembe-Tamfum, A. Trompette, B. Lina, L.O. Magnius, J.J. Chomel, H. Norder, J. Med. Virol. 79, 393 (2007)CrossRefPubMedGoogle Scholar
  22. 22.
    K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar, Mol. Biol. Evol. 30, 2725 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    H.L. Wen, L.Y. Si, X.J. Yuan, S.B. Hao, F. Gao, F.L. Chu, C.X. Sun, Z.Y. Wang, Virol J 10, 115 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    J.H. Song, K. Park, A. Shim, B.E. Kwon, J.H. Ahn, Y.J. Choi, J.K. Kim, S.G. Yeo, K. Yoon, H.J. Ko, Osong Public Heal Res Perspect 6, 52 (2015)CrossRefGoogle Scholar
  25. 25.
    M. Xu, L. Su, L. Cao, H. Zhong, N. Dong, J. Xu, BMC Infect. Dis. 13, 489 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    J.F. Chen, R.S. Zhang, X.H. Ou, F.M. Chen, B.C. Sun, Int J Infect Dis 28, 17 (2014)CrossRefPubMedGoogle Scholar
  27. 27.
    S. Singh, V.T. Chow, M.C. Phoon, K.P. Chan, C.L. Poh, J. Clin. Microbiol. 40, 2823 (2002)CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    C.C. Liu, Y.H. Chow, P. Chong, M. Klein, Vaccine 32, 6177 (2014)CrossRefPubMedGoogle Scholar
  29. 29.
    A. Muehlenbachs, J. Bhatnagar, S.R. Zaki, J Pathol 235, 217 (2015)CrossRefPubMedGoogle Scholar
  30. 30.
    C. Tapparel, F. Siegrist, T.J. Petty, L. Kaiser, Infect Genet Evol 14, 282 (2013)CrossRefPubMedGoogle Scholar
  31. 31.
    B.A. Brown, K. Maher, M.R. Flemister, P. Naraghi-Arani, M. Uddin, M.S. Oberste, M.A. Pallansch, J. Gen. Virol. 90, 1713 (2009)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Yi Xu
    • 1
  • Yisuo Sun
    • 2
  • Jinmin Ma
    • 2
  • Shuru Zhou
    • 1
    • 3
  • Wei Fang
    • 2
  • Jiawei Ye
    • 1
  • Limei Tan
    • 1
  • Jingkai Ji
    • 2
  • Dan Luo
    • 1
  • Liqiang Li
    • 2
  • Jiandong Li
    • 2
  • Chunxiao Fang
    • 1
  • Na Pei
    • 2
  • Shuo Shi
    • 2
  • Xin Liu
    • 2
  • Hui Jiang
    • 2
  • Sitang Gong
    • 1
    Email author
  • Xun Xu
    • 2
    Email author
  1. 1.Department of Infectious DiseaseGuangzhou Women and Children’s Medical CenterGuangzhouChina
  2. 2.BGI-ShenzhenShenzhenChina
  3. 3.Guangzhou Institute of PediatricsGuangzhou Women and Children’s Medical CenterGuangzhouChina

Personalised recommendations