Review of Beet pseudoyellows virus genome structure built the consensus genome organization of cucumber strains and highlighted the unique feature of strawberry strain
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The complete nucleotide sequences of Beet pseudoyellows virus (BPYV)-MI (cucumber isolate; Matsuyama, Idai) genomic RNAs 1 and 2 were determined and compared with the previously sequenced Japanese cucumber strain (BPYV-JC) and a strawberry strain (BPYV-S). The RNA 2 of BPYV-MI showed 99 % nucleotide sequence identity with both BPYV-JC and -S having highly conserved eight ORFs. In contrast, the RNA1 of BPYV-MI showed sequence identities of 98 and 86 % with BPYV-JC and -S, respectively. Phylogenetic analysis of RNA-dependent RNA polymerase (RdRp) coding sequences from three fully sequenced BPYV strains and five partially sequenced cucurbit-infecting BPYV strains from Japan and South Africa has shown that cucurbit-infecting strains are closer to each other than to BPYV-S. In addition, the strawberry strain BPYV-S has an ORF2 in the downstream of RdRp gene in RNA1, but all the cucumber strains, BPYV-JC, -MI, and those from South Africa, lacked the ORF2 of RNA1, highlighting the difference between common BPYV cucumber strains and a unique strawberry strain.
KeywordsBPYV Crinivirus Cucumber Host adaptation Strawberry
The authors are grateful to Reiko Tomita and Kazue Obara for technical assistance, and Dennis Murphy for English editing. This study was supported in part by the United Graduate School of Agricultural Sciences, Ehime University (MSA and KK), JSPS KAKENHI Grant Number 26292026 and 15K14664 (KK), the Program for Promotion of Basic and Applied Researches in Bio-oriented Industry (MNi), JSPS KAKENHI Grant Number 24580065 (MNi), and Ehime Prefecture (MNa). MSA is supported by the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT).
This study was funded by the National University Corporation Ehime University; Japan Society for the Promotion of Science (Grant Numbers 26292026 and 15K14664 to KK, and 24580065 to MNi), National Agriculture and Food Research Organization, and Ehime Prefecture government.
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.