Genome sequence analysis of two South African isolates of Moroccan watermelon mosaic virus infecting cucurbits
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Moroccan watermelon mosaic virus (MWMV) has been prevalent in cucurbits in the Republic of South Africa (RSA) since it was first reported in 1987. However, full genome studies of the South African isolates have never been conducted previously. The full genome of two MWMV isolates infecting cucurbits (Cucurbita pepo L.) in the province of KwaZulu-Natal, RSA, was compared with the genome of the Tunisian isolate in this communication. The genome sequences of the RSA MWMV isolates were elucidated using next-generation sequencing and Sanger sequencing. The analyses performed included nucleotide and amino acid sequence comparison, determination of the genetic distances, detection of potential recombination, and phylogeny. The genome sequences of the RSA MWMV isolates were found to be 9719 nucleotides long, excluding the poly(A) tail. Sequence homology, genetic distances, and phylogenetic analyses indicated close relationships between the RSA isolates. This record will contribute to building up the MWMV isolate sequences from the different countries where the virus occurs, a useful step toward understanding MWMV evolution.
KeywordsPotyvirus Phylogenomics ssRNA virus Next-generation sequencing Southern Africa Cucurbit
The authors are grateful to Mr Jonathan Featherston and Ms Thulile Faith Nhlapo, from the ARC-BTP, for their technical guidance on NGS that they provided. Sanger Sequencing was performed at Inqaba Biotechnical Industries (Pty) Ltd (Pretoria, RSA).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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