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Virus Genes

, Volume 52, Issue 6, pp 848–854 | Cite as

Epidemiological investigation of Middle East respiratory syndrome coronavirus in dromedary camel farms linked with human infection in Abu Dhabi Emirate, United Arab Emirates

  • Salama Al MuhairiEmail author
  • Farida Al Hosani
  • Yassir M. Eltahir
  • Mariam Al Mulla
  • Mohammed F. Yusof
  • Wissam S. Serhan
  • Farouq M. Hashem
  • Elsaeid A. Elsayed
  • Bahaaeldin A. Marzoug
  • Assem S. Abdelazim
Article

Abstract

The objective of this research was to investigate the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) infection primarily in dromedary camel farms and the relationship of those infections with infections in humans in the Emirate of Abu Dhabi. Nasal swabs from 1113 dromedary camels (39 farms) and 34 sheep (1 farm) and sputum samples from 2 MERS-CoV-infected camel farm owners and 1 MERS-CoV-infected sheep farm owner were collected. Samples from camels and humans underwent real-time reverse-transcription quantitative PCR screening to detect MERS-CoV. In addition, sequencing and phylogenetic analysis of partially characterized MERS-CoV genome fragments obtained from camels were performed. Among the 40 farms, 6 camel farms were positive for MERS-CoV; the virus was not detected in the single sheep farm. The maximum duration of viral shedding from infected camels was 2 weeks after the first positive test result as detected in nasal swabs and in rectal swabs obtained from infected calves. Three partial camel sequences characterized in this study (open reading frames 1a and 1ab, Spike1, Spike2, and ORF4b) together with the corresponding regions of previously reported MERS-CoV sequence obtained from one farm owner were clustering together within the larger MERS-CoV sequences cluster containing human and camel isolates reported for the Arabian Peninsula. Data provided further evidence of the zoonotic potential of MERS-CoV infection and strongly suggested that camels may have a role in the transmission of the virus to humans.

Keywords

Middle East respiratory syndrome coronavirus Dromedary camel Zoonosis 

Notes

Compliance with ethical standards

Conflict of Interest

Authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the Abu Dhabi Health Authority and Abu Dhabi Food Control Authority ethical committees.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Salama Al Muhairi
    • 1
    Email author
  • Farida Al Hosani
    • 2
  • Yassir M. Eltahir
    • 3
  • Mariam Al Mulla
    • 2
  • Mohammed F. Yusof
    • 1
  • Wissam S. Serhan
    • 1
  • Farouq M. Hashem
    • 1
  • Elsaeid A. Elsayed
    • 4
  • Bahaaeldin A. Marzoug
    • 4
  • Assem S. Abdelazim
    • 4
  1. 1.Veterinary Laboratories Division, Animal Wealth SectorAbu Dhabi Food Control AuthorityAbu DhabiUnited Arab Emirates
  2. 2.Department of Communicable Diseases, Public Health and ResearchAbu Dhabi Health AuthorityAbu DhabiUnited Arab Emirates
  3. 3.Epidemiology Section, Animal Wealth SectorAbu Dhabi Food Control AuthorityAbu DhabiUnited Arab Emirates
  4. 4.Veterinary services Section, Animal Wealth SectorAbu Dhabi Food Control AuthorityAbu DhabiUnited Arab Emirates

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