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Bufavirus Protoparvovirus in feces of wild rats in China

Abstract

Bufavirus (BuV) was first discovered from feces of children with acute diarrhea. It was subsequently detected from several animal species including shrews, bats, and nonhuman primates. In this study, we identified a novel Protoparvovirus, designated RatBuV, from the intestinal contents of wild rats using viral metagenomics. The near complete genome was 4643 nt encoding NS1, VP1, and VP2 proteins. Phylogenetic analysis over the complete genome showed that RatBuV clustered with Mpulungu BuV from shrews. Sequence analysis indicated that the putative protein sequences of NS1, VP1, and VP2 of RatBuV shared identities of 50.6–77.2, 48.3–77.3, and 47.1–78.3 %, respectively, with those of human BuVs, MpBuV, and WUHARV parvovirus, suggesting RatBuV belongs to a new species of Protoparvovirus. Our epidemiologic study indicated that the prevalence rate of RatBuV in the cohort of 40 wild rats is 12.5 % (5/40), which is higher than that of BuV in humans in a previous study.

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Acknowledgments

This work was partly supported by the Natural Science Foundation of Jiangsu Province No. BK20130502 and BK20140537, the National Natural Science Foundation of China No. 31302107, the Professional Research Foundation for Advanced Talents of Jiangsu University Nos. 12JDG085, 13JDG025, and 13JDG087, the Postdoctoral Foundation of Jiangsu Province No. 1302058C, and China Postdoctoral Special Foundation No. 2015T80503.

Author information

Correspondence to Wen Zhang.

Additional information

Shixing Yang and Dawei Liu have contributed equally to this work.

Edited by Juergen A. Richt.

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Yang, S., Liu, D., Wang, Y. et al. Bufavirus Protoparvovirus in feces of wild rats in China. Virus Genes 52, 130–133 (2016). https://doi.org/10.1007/s11262-015-1262-1

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Keywords

  • Bufavirus
  • Wild rats
  • Phylogentic analysis
  • Genome structure