Advertisement

Virus Genes

, Volume 51, Issue 2, pp 306–309 | Cite as

Analysis of genome comparison of two Indian isolates of Cowpea aphid-borne mosaic virus from India

  • Ritesh Mishra
  • Rakesh Kumar Verma
  • Rajarshi Kumar Gaur
Article

Abstract

The complete sequence of two Cowpea aphid-borne mosaic virus (CABMV) isolates (RR3 and RR4) from India was determined. Phylogenetic analysis showed that both isolates showed different closeness with other isolates of CABMV. CABMV-RR3 showed maximum identity of 99 % with CABMV-BR1 from Brazil at nucleotide and protein levels, whereas CABMV-RR4 showed identity of 73 and 95 % with CABMV-Z isolate from Zimbabwe at nucleotide and protein levels respectively. Similarity identity matrix revealed 69 % identity at nucleotide level and 91 % at protein level with each other. Recombination breakpoint detection showed that CABMV-MG-Avr from Brazil and CABMV-Z from Zimbabwe act as major parents in our isolates RR3 and RR4, respectively.

Keywords

Potyvirus Cowpea aphid-borne mosaic virus Complete sequence Polyprotein Recombination 

Notes

Acknowledgments

The authors are thankful to the Department of Biotechnology, GOI, India (BT/PR14902/BRB/10/889/2010) for financial support.

Supplementary material

11262_2015_1227_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 kb)

References

  1. 1.
    W.G. Dougherty, J.C. Carrington, Annu. Rev. Phytopathol. 26, 123–143 (1988)CrossRefGoogle Scholar
  2. 2.
    K.R. Bock, Ann. Appl. Biol. 74(1), 75–83 (1973)CrossRefPubMedGoogle Scholar
  3. 3.
    R. Mishra, R.K. Verma, R.K. Gaur, J. Plant Pathol. (2015). doi: 10.1007/s10327-014-0576-5 Google Scholar
  4. 4.
    E.W. Kitajima, B.K. de Alcantara, P.M. Madureira, P. Alfenas-Zerbini, J.A.M. Rezende, F.M. Zerbini, Arch. Virol. 153, 743–747 (2008)CrossRefPubMedGoogle Scholar
  5. 5.
    G. Pio-Ribeiro, S.S. Pappu, H.R. Pappu, G.P. Andrade, D.V.R. Reddy, Plant Dis. 84, 760–766 (2000)CrossRefGoogle Scholar
  6. 6.
    A.V.S. Nascimento, E.N. Santana, A.S.K. Braz, P.F. Alfenas, G. Pio-Ribeiro, G.P. Andrade, M.G. Carvalho, F.M. Zerbini, Arch. Virol. 151, 1797–1809 (2006)CrossRefPubMedGoogle Scholar
  7. 7.
    J.D. Ehlers, A.E. Hall, Field Crop. Res. 35, 187–204 (1997)CrossRefGoogle Scholar
  8. 8.
    K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)PubMedCentralCrossRefPubMedGoogle Scholar
  9. 9.
    T.A. Hall, Nucleic Acids Symp. Ser. 41, 95–98 (1991)Google Scholar
  10. 10.
    D.P. Martin, P. Lemey, M. Lott, V. Moulton, D. Posada, P. Lefeuvre, Bioinformatics 26, 2462–2463 (2010)PubMedCentralCrossRefPubMedGoogle Scholar
  11. 11.
    J. Verchot, E.V. Koonin, J.C. Carrington, Virology 190, 527–535 (1991)CrossRefGoogle Scholar
  12. 12.
    S. Blanc, J.J. Lopez Moya, R. Wang, S. Garcia Lampasona, D.W. Thornbury, T.P. Pirone, R.Y. Wang, Virology 231, 141–147 (1997)CrossRefPubMedGoogle Scholar
  13. 13.
    C. Desbiez, M. Girard, H. Lecoq, Arch. Virol. 155, 397–401 (2010)CrossRefPubMedGoogle Scholar
  14. 14.
    A. Gal-On, Phytopathology 90, 467–473 (2000)CrossRefPubMedGoogle Scholar
  15. 15.
    R.M. Eagles, E. Balmorimelian, D.L. Beck, R.C. Gardner, R.L.S. Forster, Eur. J. Biochem. 224, 677–684 (1994)CrossRefPubMedGoogle Scholar
  16. 16.
    A. Fernandez, S. Lain, J.A. Garcia, Nucleic Acids Res. 23, 1327–1332 (1995)PubMedCentralCrossRefPubMedGoogle Scholar
  17. 17.
    S. Laín, M.T. Martin, J.L. Riechmann, J.A. García, J. Virol. 65, 1–6 (1991)PubMedCentralCrossRefPubMedGoogle Scholar
  18. 18.
    Y. Hong, A.G. Hunt, Virology 226, 146–151 (1996)CrossRefPubMedGoogle Scholar
  19. 19.
    P.L. Atreya, J.J. Lopez-Moya, M.H. Chu, C.D. Atreya, T.P. Pirone, J. Gen. Virol. 76, 265–270 (1995)CrossRefPubMedGoogle Scholar
  20. 20.
    D.R. Barros, A.Z. Poliane Jr., J.E.A. Beserra, T.F.S. Antunes, F.M. Zerbini, Arch. Virol. 156, 1085–1091 (2011)CrossRefPubMedGoogle Scholar
  21. 21.
    M.J. Roossinck, Annu. Rev. Phytopathol. 35, 191–209 (1997)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ritesh Mishra
    • 1
  • Rakesh Kumar Verma
    • 1
  • Rajarshi Kumar Gaur
    • 1
  1. 1.Department of Science, College of Arts, Science and HumanitiesMody University of Science and TechnologySikarIndia

Personalised recommendations