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Virus Genes

, Volume 51, Issue 1, pp 45–50 | Cite as

Isolation of buffalo poxvirus from clinical case and variations in the genetics of the B5R gene over fifty passages

  • Mohsan Ullah Goraya
  • Zafar ul Ahsan Qureshi
  • Muhammad Abbas
  • Muhammad Ashraf
  • Muhammad Munir
Article

Abstract

Outbreaks of buffalopox affect udder and teats, which may ultimately lead to mastitis in dairy buffalo and can significantly compromise the production. In this study, we report isolation of buffalo poxvirus and sequence analysis of the B5R gene collected from the buffalo clinically suspected to be poxvirus infected. The virus was isolated on BHK-21 cell line and was passaged for 50 times, B5R gene was amplified and sequenced using gene-specific primers, and analyzed at both nucleotide and amino acid levels. Phylogenetically, the isolate can be classified close to the previously reported Pakistani and Indian isolates with certain level of differential clustering patterns. Three significant putative mutations (I2K, N64D, and K111E) were observed in the B5R protein. The K111E was common with previous human isolate from Karachi, Pakistan in 2005. These mutations differed from poxviruses reported from the neighboring countries. Some deletion mutations were observed which were recovered in upcoming passages. The K111E mutation suggests potential to cause zoonotic infection in human all over the country.

Keywords

Buffalo poxvirus B5Rgene Zoonosis Host range Phylogeny 

Notes

Acknowledgments

All the authors contributed equally in the study and have no competing interest in the manuscript.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Mohsan Ullah Goraya
    • 1
    • 2
  • Zafar ul Ahsan Qureshi
    • 3
  • Muhammad Abbas
    • 4
  • Muhammad Ashraf
    • 5
  • Muhammad Munir
    • 6
  1. 1.College of Animal SciencesFujain Agriculture and Forestry UniversityFuzhouChina
  2. 2.Uppsala UniversityUppsalaSweden
  3. 3.Veterinary Research InstituteLahorePakistan
  4. 4.Livestock and Dairy Development DepartmentGovernment of PunjabLahorePakistan
  5. 5.Institute of MicrobiologyUniversity of AgricultureFaisalabadPakistan
  6. 6.The Pirbright InstituteWokingUK

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