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Virus Genes

, Volume 50, Issue 3, pp 518–522 | Cite as

Complete genome sequence of an isolate of Potato virus X (PVX) infecting Cape gooseberry (Physalis peruviana) in Colombia

  • Pablo A. GutiérrezEmail author
  • Juan F. Alzate
  • Mauricio Marín Montoya
Article

Abstract

Transcriptome analysis of a Cape gooseberry (Physalis peruviana) plant with leaf symptoms of a mild yellow mosaic typical of a viral disease revealed an infection with Potato virus X (PVX). The genome sequence of the PVX-Physalis isolate comprises 6435 nt and exhibits higher sequence similarity to members of the Eurasian group of PVX (~95 %) than to the American group (~77 %). Genome organization is similar to other PVX isolates with five open reading frames coding for proteins RdRp, TGBp1, TGBp2, TGBp3, and CP. 5′ and 3′ untranslated regions revealed all regulatory motifs typically found in PVX isolates. The PVX-Physalis genome is the only complete sequence available for a Potexvirus in Colombia and is a new addition to the restricted number of available sequences of PVX isolates infecting plant species different to potato.

Keywords

Genomics Next generation sequencing Physalis peruviana Potexvirus 

Notes

Acknowledgments

This work was funded by Universidad Nacional de Colombia through Grant VRI: 19438 and International Foundation for Science (IFS, Sweden, Research Grant Agreement NO. C/4634-2).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11262_2015_1181_MOESM1_ESM.jpg (598 kb)
Supplementary material 1 (599 kb)
11262_2015_1181_MOESM2_ESM.fasta (6 kb)
Supplementary material 2 (7 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Pablo A. Gutiérrez
    • 1
    Email author
  • Juan F. Alzate
    • 2
  • Mauricio Marín Montoya
    • 3
  1. 1.Laboratorio de Microbiología Industrial, Facultad de CienciasUniversidad Nacional de Colombia Sede MedellínMedellínColombia
  2. 2.Centro Nacional de Secuenciación Genómica- CNSG, Facultad de Medicina, Grupo de ParasitologíaUniversidad de AntioquiaMedellínColombia
  3. 3.Laboratorio de Biología Celular y Molecular, Facultad de CienciasUniversidad Nacional de Colombia Sede MedellínMedellínColombia

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