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Virus Genes

, Volume 50, Issue 2, pp 238–244 | Cite as

Identification of NS1 domains of avian H5N1 influenza virus which influence the interaction with the NOLC1 protein

  • Chun-yu Zhu
  • Fang-liang Zheng
  • Xiao-shuang She
  • Dan Zhao
  • Ying Gu
  • Yan-ting Duan
  • Alan K. Chang
  • Hong-sheng LiuEmail author
Article

Abstract

Non-structural protein 1 (NS1) is an important virulence factor encoded by influenza A virus. NS1 can interact with a variety of host cell proteins to interfere with the host innate immune response and to promote effective viral replication. Our previous work has shown that only the effector domain of NS1 (amino acid residues 74-230/237) is sufficient to interact with nucleolar and coiled-body phosphoprotein 1 (NOLC1). To investigate the exact region of NS1 that interacts with NOLC1, we used only the effector domain of NS1 and constructed various mutants having different deletions, and then tested their ability to interact with NOLC1 via pull-down assay. Only the mutant containing amino acid residues 104–200 showed positive interaction with NOLC1. To further determine the key amino acids of the NS1 effector domain which are crucial for interaction with NOLC1, several mutants containing a single amino acid substitution were made and their interaction with NOLC1 was tested. Only the mutant D120A or R195A showed reduced binding with NOLC1, suggesting that D120 and R195 were crucial to the binding of NS1 to NOLC1. This study lays the foundation for further research aiming at furthering our understanding of the interaction between NS1 and host cells.

Keywords

Influenza A virus NS1 NOLC1 

Notes

Acknowledgments

This work was supported by Fund of the Key Laboratory of Department of Education of Liaoning province (No. 2009S043). We thank the College of Life Science of Wuhan University for its support.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Chun-yu Zhu
    • 1
  • Fang-liang Zheng
    • 1
  • Xiao-shuang She
    • 1
  • Dan Zhao
    • 1
  • Ying Gu
    • 1
  • Yan-ting Duan
    • 1
  • Alan K. Chang
    • 1
  • Hong-sheng Liu
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning ProvinceCollege of Life Science of Liaoning UniversityShenyangPeople’s Republic of China
  2. 2.Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province, Academy of ScienceLiaoning UniversityShenyangPeople’s Republic of China

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