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Virus Genes

, Volume 49, Issue 3, pp 373–382 | Cite as

miR-27a suppresses EV71 replication by directly targeting EGFR

  • Lianglu Zhang
  • Xiong Chen
  • Yingying Shi
  • Bingfei Zhou
  • Chen Du
  • Yongjuan Liu
  • Song Han
  • Jun Yin
  • Biwen Peng
  • Xiaohua HeEmail author
  • Wanhong LiuEmail author
Article

Abstract

Enterovirus 71 (EV71), a major causative agent of hand, foot, and mouth disease, has broken out several times and was accompanied by neurological disease. microRNAs, a class of small non-coding RNAs that are approximately 20 nucleotides long, play important roles in the regulation of various biological processes, including antiviral defense. However, the roles of miRNAs in EV71 replication and pathogenesis are not well understood. In this study, we found that the expression of miR-27a was significantly decreased in EV71-infected cells. Interestingly, the over-expression of miR-27a could inhibit EV71 replication, as measured by virus titration, qPCR, and Western blotting. We identified EGFR mRNA is a bona fide target of miR-27a by computational analysis and luciferase reporter assays. Furthermore, miR-27a could decrease EGFR expression, as measured by qPCR and Western blotting. Moreover, the inhibition of EGFR expression by miR-27a decreased the phosphorylation of Akt and ERK, which facilitate EV71 replication. These results suggest that miR-27a may have antiviral activity against EV71 by inhibiting EGFR.

Keywords

EV71 miR-27a EGFR Virus replication Inhibition 

Notes

Acknowledgments

We thank Prof. Zan Huang, Qiangson Tong for material assistance. This work was supported by the National Natural Sciences Foundation of China (No. 81171577, 81371790, 81371422 and 31100896), the Fundamental Research Funds for the Central Universities of China, and Translational Medical Research Fund of Wuhan University School of Medicine.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Lianglu Zhang
    • 1
  • Xiong Chen
    • 1
  • Yingying Shi
    • 1
    • 2
  • Bingfei Zhou
    • 1
  • Chen Du
    • 1
  • Yongjuan Liu
    • 1
  • Song Han
    • 1
  • Jun Yin
    • 1
  • Biwen Peng
    • 1
    • 3
  • Xiaohua He
    • 1
    • 3
    Email author
  • Wanhong Liu
    • 1
    • 2
    Email author
  1. 1.Pathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical SciencesWuhan UniversityWuhanChina
  2. 2.Hubei Province Key Laboratory of Allergy and ImmunologyWuhanChina
  3. 3.Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical SciencesWuhan UniversityWuhanChina

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