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Virus Genes

, Volume 49, Issue 1, pp 100–105 | Cite as

Complete sequence and phylogenetic analysis of a porcine sapovirus strain isolated from western China

  • Wei Liu
  • Bin Yang
  • Enli Wang
  • Jixing Liu
  • Xi Lan
Article

Abstract

Sapovirus (SaV) is a type of calicivirus that can cause acute viral gastroenteritis in humans and animals. SaVs have been found in several mammalian species, including humans, pigs, minks, dogs, and bats. Porcine sapovirus (PoSaV) was first identified in 1980 in the United States and has been found to be circulating throughout China in recent years. In this study, the complete genomic characterization of PoSaV CH430, first found in west China, was reported and analyzed. The genome was 7,342 bp excluding the 30 nt poly(A) tail at the 3′ terminus and comprised two major open reading frames. Comprehensive evolutionary and phylogenetic analyses indicated that the CH430 strain belongs to genotype III SaVs. However, this particular isolate and DG24 strain occupied an independent branch of the phylogenetic tree we generated, indicating that they could form a separate subgenotype in the near future. We predicted the cleavage sites for the ORF1 polyprotein located at Q56/G57, Q310/A311, E649/A650, E934/A935, E1047/G1048, and E1712/A1713, separately. This is the first PoSaV strain isolated from western China to be fully sequenced and characterized. It provided a reliable experimental basis for studying the genetic nature of emerging PoSaVs.

Keywords

Porcine Sapovirus Complete genome Sequence analysis Northwest China 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Wei Liu
    • 1
  • Bin Yang
    • 1
  • Enli Wang
    • 1
    • 2
  • Jixing Liu
    • 1
  • Xi Lan
    • 1
  1. 1.State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Grazing Animal Diseases, Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesLanzhouChina
  2. 2.College of Veterinary MedicineGansu Agricultural UniversityLanzhouChina

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