Virus Genes

, Volume 48, Issue 3, pp 557–561 | Cite as

Isolation and molecular characterization of a distinct begomovirus and its associated betasatellite infecting Hedyotis uncinella (Hook. et Arn.) in Vietnam

  • Zhenguo Du
  • Meixiang Chen
  • Zhen Wang
  • Yong Liu
  • Songbai ZhangEmail author
  • Zifu HeEmail author


A begomovirus isolate VN1 associated with symptomatic Hedyotis uncinella Hook. et Arn. from Vietnam was characterized. The virus, which we provisionally name H. uncinella yellow mosaic virus (HUYMV), has a monopartite genome of 2,749 nucleotides (nts). Pairwise comparisons of DNA-A sequences showed that HUYMV had a maximum nt sequence identity with Soybean crinkle leaf virus (SbLCV) and Premna leaf curl virus (PLCuV) at 82.1 and 81.9 %, respectively, which are less than the 89 % identity in the complete genome, which has been used as the threshold value for demarcation of species in the genus Begomovirus, the family Geminiviridae. One recombination event was detected for HUYMV, which involves an unknown begomovirus as the major parent and Tomato leaf curl Philippines virus (ToLCPV) as the minor parent, with nt 2163 and nt 2452 as the beginning and ending breakpoints, respectively. A betasatellite was found to be associated with HUYMV. The betasatellite showed the highest nt sequence identity (70 %) with Tomato leaf curl Philippine betasatellite—[Philippines:Laguna2:2006]. The name H. uncinella yellow mosaic betasatellite [Vietnam: Binh Dinh: 2013] was proposed for the betasatellite.


Begomovirus New species Wild plant  Hedyotis uncinella Hook. et Arn. 



This study was supported by the Special Fund for Agro-scientific Research in the Public Interest (No. 201303028), the Foundation of President of GAAS of China (No. 201207), the Sino-Asean network for early-warning and management of major alien invasive pests (2013J4500032), and the National Natural Science Foundation of China (Grant No. 31301638). The authors are grateful to Dr. Sharon van Brunschot at The University of Queensland for her help in revising the article.

Supplementary material

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  1. 1.
    M.J. Adams, A.M.Q. King, E.B. Carstens, Arch. Virol. 158, 2023–2030 (2013)PubMedCrossRefGoogle Scholar
  2. 2.
    R.W. Briddon, S.E. Bull, S. Mansoor, I. Amin, P.G. Markham, Mol. Biotechnol. 20, 315–318 (2002)PubMedCrossRefGoogle Scholar
  3. 3.
    R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, C.M. Fauquet, Arch. Virol. 153, 763–781 (2008)PubMedCrossRefGoogle Scholar
  4. 4.
    J.K. Brown, C.M. Fauquet, R.W. Briddon, M. Zerbini, E. Moriones, J. Navas-Castillo, in Virus taxonomy, ninth report of the ICTV, ed. by A.M.Q. King, M.J. Adams, E.B. Carstens, E.J. Lefkowitz (Academic Press, London, 2012), pp. 351–373Google Scholar
  5. 5.
    C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X.P. Zhou, Arch. Virol. 153, 783–821 (2008)PubMedCrossRefGoogle Scholar
  6. 6.
    J. Felsenstein, Evolution 39, 783–791 (1985)CrossRefGoogle Scholar
  7. 7.
    M.R. Fontenelle, D.F. Luz, A.P. Gomes, L.H. Florentino, F.M. Zerbini, E.P. Fontes, Virus Res. 126, 262–267 (2007)PubMedCrossRefGoogle Scholar
  8. 8.
    C. Ha, S. Coombs, P. Revill, R. Harding, M. Vu, J. Dale, J. Gen. Virol. 89, 312–326 (2008)PubMedCrossRefGoogle Scholar
  9. 9.
    L. Hanley-Bowdoin, S.B. Settlage, B.M. Orozco, S. Nagar, D. Robertson, Crit. Rev. Plant Sci. 18, 71–106 (1999)CrossRefGoogle Scholar
  10. 10.
    B.D. Harrison, Y.L. Liu, S. Khalid, S. Hameed, G.W. Otim-Nape, D.J. Robinson, Ann. Appl. Biol. 130, 61–75 (1997)CrossRefGoogle Scholar
  11. 11.
    Z.F. He, H. Yu, F.F. Luo, Virol. Sin. 19, 67–69 (2004)Google Scholar
  12. 12.
    A. Kheyr-Pour, K. Bananej, G.A. Dafalla, P. Caciagli, E. Noris, A. Ahoonmanesh, H. Lecoq, B. Gronenborn, Phytopathology 90, 629–635 (2000)PubMedCrossRefGoogle Scholar
  13. 13.
    D.P. Martin, P. Lemey, M. Lott, V. Moulton, D. Posada, P. Lefeuvre, Bioinformatics 26, 2462–2463 (2010)PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    A.S. Moffat, Science 286, 1835 (1999)CrossRefGoogle Scholar
  15. 15.
    B. Muhire, D.P. Martin, J.K. Brown, J. Navas-Castillo, E. Moriones, F.M. Zerbini, R. Rivera-Bustamante, V.G. Malathi, R.W. Briddon, A. Varsani, Arch. Virol. 158, 1411–1424 (2013)PubMedCrossRefGoogle Scholar
  16. 16.
    N. Navot, E. Pichersky, M. Zeidan, D. Zamir, H. Czosnek, Virology 185, 151–161 (1991)PubMedCrossRefGoogle Scholar
  17. 17.
    M.S. Nawaz-ul-Rehman, C.M. Fauquet, FEBS Lett. 583, 1825–1832 (2009)PubMedCrossRefGoogle Scholar
  18. 18.
    N. Saitou, M. Nei, Mol. Biol. Evol. 4, 406–425 (1987)PubMedGoogle Scholar
  19. 19.
    K. Ooi, S. Oshita, I. Ishii, T. Yahara, J. Plant Res. 110, 247–257 (1997)CrossRefGoogle Scholar
  20. 20.
    R. Prajapat, A. Marwal, R.K. Gaur, Arch. Phytopathol. Plant Prot. (2013). doi: 10.1080/03235408.2013.805497 Google Scholar
  21. 21.
    V. Raghavan, P.S. Malik, N.R. Choudhury, S.K. Mukherjee, J. Virol. 78, 2405–2413 (2004)PubMedCentralPubMedCrossRefGoogle Scholar
  22. 22.
    X. She, Z. He, J.K. Brown, Arch. Virol. 158, 2428 (2013)CrossRefGoogle Scholar
  23. 23.
    D.N. Shepherd, D.P. Martin, E. van der Walt, K. Dent, A. Varsani, E.P. Rybicki, Mol. Plant Pathol. 11, 1–12 (2010)PubMedCrossRefGoogle Scholar
  24. 24.
    D.C. Stenger, G.N. Revington, M.C. Stevenson, D.M. Bisaro, Proc. Natl. Acad. Sci. USA 88, 8029–8030 (1991)PubMedCentralPubMedCrossRefGoogle Scholar
  25. 25.
    K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)PubMedCentralPubMedCrossRefGoogle Scholar
  26. 26.
    A. Varma, V.G. Malathi, Ann. Appl. Biol. 142, 145–164 (2003)CrossRefGoogle Scholar
  27. 27.
    S.D. Wyatt, J.K. Brown, Phytopathology 86, 1288–1293 (1996)CrossRefGoogle Scholar
  28. 28.
    X.P. Zhou, Annu. Rev. Phytopathol. 51, 357–381 (2013)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.College of AgricultureYangtze UniversityJingzhouChina
  2. 2.Plant Protection Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouChina
  3. 3.Guangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina
  4. 4.Plant Protection Research InstituteHunan Academy of Agricultural SciencesChangshaChina

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